DE602005020691D1 - Rna-amplifikation auf ligationsbasis - Google Patents

Rna-amplifikation auf ligationsbasis

Info

Publication number
DE602005020691D1
DE602005020691D1 DE602005020691T DE602005020691T DE602005020691D1 DE 602005020691 D1 DE602005020691 D1 DE 602005020691D1 DE 602005020691 T DE602005020691 T DE 602005020691T DE 602005020691 T DE602005020691 T DE 602005020691T DE 602005020691 D1 DE602005020691 D1 DE 602005020691D1
Authority
DE
Germany
Prior art keywords
nucleic acid
stranded region
ligation
basis
rna amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005020691T
Other languages
English (en)
Inventor
Rohini Dhulipala
Scott R Duthie
John R Nelson
Gregory A Grossmann
Anuradha Sekher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Global Life Sciences Solutions USA LLC
Original Assignee
GE Healthcare Bio Sciences AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB, GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences AB
Publication of DE602005020691D1 publication Critical patent/DE602005020691D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
DE602005020691T 2004-12-23 2005-12-22 Rna-amplifikation auf ligationsbasis Active DE602005020691D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US63893704P 2004-12-23 2004-12-23
US68566105P 2005-05-27 2005-05-27
PCT/US2005/046800 WO2006071776A2 (en) 2004-12-23 2005-12-22 Ligation-based rna amplification

Publications (1)

Publication Number Publication Date
DE602005020691D1 true DE602005020691D1 (de) 2010-05-27

Family

ID=36499164

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005020691T Active DE602005020691D1 (de) 2004-12-23 2005-12-22 Rna-amplifikation auf ligationsbasis

Country Status (9)

Country Link
US (2) US20080003602A1 (de)
EP (1) EP1836302B1 (de)
JP (1) JP5420174B2 (de)
AT (1) ATE464381T1 (de)
AU (1) AU2005322131B2 (de)
CA (1) CA2592425C (de)
DE (1) DE602005020691D1 (de)
DK (1) DK1836302T3 (de)
WO (1) WO2006071776A2 (de)

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CN117056774A (zh) 2016-11-08 2023-11-14 贝克顿迪金森公司 用于细胞标记分类的方法
US11164659B2 (en) 2016-11-08 2021-11-02 Becton, Dickinson And Company Methods for expression profile classification
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US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11807623B2 (en) 2017-11-30 2023-11-07 Arrakis Therapeutics, Inc. Nucleic acid-binding photoprobes and uses thereof
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US11773441B2 (en) 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
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JP2022511398A (ja) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
EP3877520A1 (de) 2018-11-08 2021-09-15 Becton Dickinson and Company Ganze transkriptomanalyse von einzelzellen mittels random-priming
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
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US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
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Also Published As

Publication number Publication date
CA2592425A1 (en) 2006-07-06
AU2005322131A1 (en) 2006-07-06
WO2006071776A2 (en) 2006-07-06
CA2592425C (en) 2014-06-17
DK1836302T3 (da) 2010-08-02
ATE464381T1 (de) 2010-04-15
JP2008525038A (ja) 2008-07-17
WO2006071776A3 (en) 2006-09-14
EP1836302A2 (de) 2007-09-26
US10704042B2 (en) 2020-07-07
JP5420174B2 (ja) 2014-02-19
EP1836302B1 (de) 2010-04-14
US20180320172A1 (en) 2018-11-08
AU2005322131B2 (en) 2011-11-10
US20080003602A1 (en) 2008-01-03

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