ATE464381T1 - Rna-amplifikation auf ligationsbasis - Google Patents
Rna-amplifikation auf ligationsbasisInfo
- Publication number
- ATE464381T1 ATE464381T1 AT05855371T AT05855371T ATE464381T1 AT E464381 T1 ATE464381 T1 AT E464381T1 AT 05855371 T AT05855371 T AT 05855371T AT 05855371 T AT05855371 T AT 05855371T AT E464381 T1 ATE464381 T1 AT E464381T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- stranded region
- ligation
- rna amplification
- based rna
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 150000007523 nucleic acids Chemical group 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US63893704P | 2004-12-23 | 2004-12-23 | |
| US68566105P | 2005-05-27 | 2005-05-27 | |
| PCT/US2005/046800 WO2006071776A2 (en) | 2004-12-23 | 2005-12-22 | Ligation-based rna amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE464381T1 true ATE464381T1 (de) | 2010-04-15 |
Family
ID=36499164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05855371T ATE464381T1 (de) | 2004-12-23 | 2005-12-22 | Rna-amplifikation auf ligationsbasis |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20080003602A1 (de) |
| EP (1) | EP1836302B1 (de) |
| JP (1) | JP5420174B2 (de) |
| AT (1) | ATE464381T1 (de) |
| AU (1) | AU2005322131B2 (de) |
| CA (1) | CA2592425C (de) |
| DE (1) | DE602005020691D1 (de) |
| DK (1) | DK1836302T3 (de) |
| WO (1) | WO2006071776A2 (de) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| US8039214B2 (en) * | 2007-06-29 | 2011-10-18 | Cellscript, Inc. | Synthesis of tagged nucleic acids |
| GB0807321D0 (en) * | 2008-04-22 | 2008-05-28 | Oxford Gene Tech Ip Ltd | Analysis of nucleic acids |
| JP2011522552A (ja) * | 2008-06-13 | 2011-08-04 | リボックス・ゲーエムベーハー | 化学的に修飾されたrnaの酵素的合成のための方法 |
| US8753868B2 (en) | 2008-08-04 | 2014-06-17 | General Electric Company | Method and system for selective isolation of target biological molecules in a general purpose system |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US20140329700A1 (en) * | 2011-08-23 | 2014-11-06 | Rutgers, The State University Of New Jersey | Methods of isolating rna and mapping of polyadenylation isoforms |
| EP2820174B1 (de) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Verfahren und verwendung für molekulare tags |
| US10941396B2 (en) * | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| GB201217770D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Biological probes and the use thereof |
| EP3988671A1 (de) * | 2013-02-20 | 2022-04-27 | Emory University | Zusammensetzungen zur sequenzierung von nukleinsäuren in mischungen |
| ES2711168T3 (es) | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| US10697010B2 (en) | 2015-02-19 | 2020-06-30 | Becton, Dickinson And Company | High-throughput single-cell analysis combining proteomic and genomic information |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| CN107580632B (zh) | 2015-04-23 | 2021-12-28 | 贝克顿迪金森公司 | 用于全转录组扩增的方法和组合物 |
| PL4108769T3 (pl) | 2015-05-29 | 2024-02-05 | CureVac Manufacturing GmbH | Sposób wytwarzania i oczyszczania rna obejmujący co najmniej jeden etap filtracji o przepływie stycznym |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| CN108026524A (zh) | 2015-09-11 | 2018-05-11 | 赛卢拉研究公司 | 用于核酸文库标准化的方法和组合物 |
| EP3394280A1 (de) * | 2015-12-23 | 2018-10-31 | CureVac AG | Verfahren für rna-in-vitro-transkription mittels eines puffers mit dicarboxylsäure oder tricarbonsäure oder einem salz davon |
| ES2956757T3 (es) | 2016-05-02 | 2023-12-27 | Becton Dickinson Co | Codificación con códigos de barras moleculares precisa |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| EP3465502B1 (de) | 2016-05-26 | 2024-04-10 | Becton, Dickinson and Company | Verfahren zur anpassung des zählens von molekularen kennzeichen |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CA3034924C (en) | 2016-09-26 | 2025-11-18 | Becton, Dickinson And Company | MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES |
| CN117056774B (zh) | 2016-11-08 | 2026-01-27 | 贝克顿迪金森公司 | 用于细胞标记分类的方法 |
| CN109952612B (zh) | 2016-11-08 | 2023-12-01 | 贝克顿迪金森公司 | 用于表达谱分类的方法 |
| CN110573253B (zh) | 2017-01-13 | 2021-11-02 | 赛卢拉研究公司 | 流体通道的亲水涂层 |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| RU2020112854A (ru) | 2017-11-30 | 2021-12-30 | Арракис Терапьютикс, Инк. | Фотозонды, связывающие нуклеиновые кислоты, и способы их применения |
| CN111492068B (zh) | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| CA3097976A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| EP3788170B1 (de) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molekulare barcodierung auf gegenüberliegenden transkriptenden |
| WO2019236644A1 (en) * | 2018-06-05 | 2019-12-12 | Arrakis Therapeutics, Inc. | Encoded libraries and methods of use for screening nucleic acid targets |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| EP4242322B1 (de) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Mit antikörpern assoziierte oligonukleotide |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用 |
| EP4090764A1 (de) | 2020-01-13 | 2022-11-23 | Becton, Dickinson and Company | Zellerfassung unter verwendung von du-haltigen oligonukleotiden |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| EP4111168A1 (de) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bispezifische sonden zur ermöglichung der verwendung von einzelzellenproben als einzelfarbkompensationssteuerung |
| EP4150118A1 (de) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primer zur profilierung von immunrepertoire |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| EP4263859A1 (de) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Einzelzellensekretomanalyse |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA2001110A1 (en) * | 1988-11-18 | 1990-05-18 | Zvi G. Loewy | Compositions and process for amplifying and detecting nucleic acid sequences |
| US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| DE69133389T2 (de) * | 1990-09-27 | 2005-06-02 | Invitrogen Corp., Carlsbad | Direkte Klonierung von PCR amplifizierten Nukleinsäuren |
| US5658736A (en) * | 1996-01-16 | 1997-08-19 | Genetics Institute, Inc. | Oligonucleotide population preparation |
| DE69738206T2 (de) * | 1996-11-06 | 2008-07-17 | Sequenom, Inc., San Diego | DNA-Diagnostik mittels Massenspektrometrie |
| DK1712623T3 (da) * | 1997-01-21 | 2012-02-06 | Gen Hospital Corp | Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner |
| FR2761695B1 (fr) * | 1997-04-04 | 1999-09-24 | Bio Merieux | Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation |
| DE69900592T2 (de) * | 1998-02-27 | 2002-08-22 | Pamgene B.V., Den Bosch | Verfahren zur unspezifischen amplifizierung einer nukleinsäure |
| JP3883476B2 (ja) * | 1999-03-19 | 2007-02-21 | タカラバイオ株式会社 | 核酸配列の増幅方法 |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| EP1356094B1 (de) * | 2000-06-26 | 2010-01-13 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| FI115139B (fi) * | 2001-01-10 | 2005-03-15 | Valtion Teknillinen | Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen |
| US20030104432A1 (en) * | 2001-07-27 | 2003-06-05 | The Regents Of The University Of California | Methods of amplifying sense strand RNA |
| US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| US7927791B2 (en) * | 2002-07-24 | 2011-04-19 | Ptc Therapeutics, Inc. | Methods for identifying small molecules that modulate premature translation termination and nonsense mediated mRNA decay |
| CA2501983A1 (en) * | 2002-10-30 | 2004-05-27 | Pamgene B.V. | Improved methods for generating multiple rna copies |
| GB0228289D0 (en) * | 2002-12-04 | 2003-01-08 | Genome Inst Of Singapore Nat U | Method |
| EP1604040B1 (de) * | 2003-03-07 | 2010-10-13 | Rubicon Genomics, Inc. | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| US20060019258A1 (en) * | 2004-07-20 | 2006-01-26 | Illumina, Inc. | Methods and compositions for detection of small interfering RNA and micro-RNA |
-
2005
- 2005-12-22 AT AT05855371T patent/ATE464381T1/de active
- 2005-12-22 DK DK05855371.0T patent/DK1836302T3/da active
- 2005-12-22 WO PCT/US2005/046800 patent/WO2006071776A2/en not_active Ceased
- 2005-12-22 EP EP05855371A patent/EP1836302B1/de not_active Expired - Lifetime
- 2005-12-22 DE DE602005020691T patent/DE602005020691D1/de not_active Expired - Lifetime
- 2005-12-22 CA CA2592425A patent/CA2592425C/en not_active Expired - Lifetime
- 2005-12-22 JP JP2007548525A patent/JP5420174B2/ja not_active Expired - Lifetime
- 2005-12-22 AU AU2005322131A patent/AU2005322131B2/en not_active Expired
- 2005-12-22 US US11/722,089 patent/US20080003602A1/en not_active Abandoned
-
2018
- 2018-04-30 US US15/966,353 patent/US10704042B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006071776A2 (en) | 2006-07-06 |
| AU2005322131B2 (en) | 2011-11-10 |
| JP5420174B2 (ja) | 2014-02-19 |
| JP2008525038A (ja) | 2008-07-17 |
| DE602005020691D1 (de) | 2010-05-27 |
| DK1836302T3 (da) | 2010-08-02 |
| CA2592425A1 (en) | 2006-07-06 |
| US20080003602A1 (en) | 2008-01-03 |
| CA2592425C (en) | 2014-06-17 |
| EP1836302A2 (de) | 2007-09-26 |
| WO2006071776A3 (en) | 2006-09-14 |
| US20180320172A1 (en) | 2018-11-08 |
| AU2005322131A1 (en) | 2006-07-06 |
| EP1836302B1 (de) | 2010-04-14 |
| US10704042B2 (en) | 2020-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE464381T1 (de) | Rna-amplifikation auf ligationsbasis | |
| WO2006081284A3 (en) | Methods for quantitating small rna molecules | |
| WO2006102309A3 (en) | Methods, compositions, and kits for detection of micro rna | |
| WO2008002920A3 (en) | Methods for generating target nucleic acid sequences | |
| BR0101493B1 (pt) | mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes. | |
| WO2008143627A3 (en) | Targeted whole genome amplification method for identification of pathogens | |
| DE60332873D1 (de) | Rolling-circle rna-amplifikation | |
| WO2011106724A3 (en) | Fast pcr for str genotyping | |
| SE0401270D0 (sv) | Method for amplifying specific nucleic acids in parallel | |
| WO2005010209A3 (de) | Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren | |
| ATE516368T1 (de) | Erstellung eines echtzeit-genexpressionsprofils | |
| DE60134146D1 (de) | Detektion und amplifizierung von rna unterverwendung von zielgerichteter dna-ligation durch rna ligase | |
| WO2006099164A3 (en) | Methods for multiplex amplification | |
| WO2006074162A3 (en) | Primer generation rolling circle amplification | |
| WO2009092035A3 (en) | Methods and compositions for the analysis of biological molecules | |
| ATE330038T1 (de) | Nukleinsäure-biosensor | |
| AU2001244677A1 (en) | Method of amplifying nucleic acid by using double-stranded nucleic acid as template | |
| DK1411122T3 (da) | Fremgangsmåde til fremstilling af nukleinsyremolekyler | |
| ATE505562T1 (de) | Doppel-oligonukleotid- nukleinsäurenachweisverfahren | |
| WO2004065582A3 (en) | Amplification of dna in a hairpin structure, and applications | |
| WO2020132316A3 (en) | Methods for nucleic acid target enrichment | |
| WO2011066330A3 (en) | Selective amplification of polynucleotide sequences | |
| WO2004104229A3 (en) | Internal control nucleic acid molecule for nucleic acid amplification systems | |
| WO2003029441A1 (en) | Method of amplifying dna chip signals | |
| WO2009131683A3 (en) | Method for detecting chikungunya virus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1836302 Country of ref document: EP |