WO2005010209A3 - Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren - Google Patents

Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren Download PDF

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Publication number
WO2005010209A3
WO2005010209A3 PCT/EP2004/008363 EP2004008363W WO2005010209A3 WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3 EP 2004008363 W EP2004008363 W EP 2004008363W WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acids
reverse transcription
amplification
pool
derived
Prior art date
Application number
PCT/EP2004/008363
Other languages
English (en)
French (fr)
Other versions
WO2005010209A2 (de
Inventor
Tanja Wille
Christian Korfhage
Eric Lader
Original Assignee
Qiagen Gmbh
Tanja Wille
Christian Korfhage
Eric Lader
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh, Tanja Wille, Christian Korfhage, Eric Lader filed Critical Qiagen Gmbh
Priority to AU2004259851A priority Critical patent/AU2004259851A1/en
Priority to EP04763506A priority patent/EP1651776A2/de
Priority to US10/565,989 priority patent/US20060281092A1/en
Priority to JP2006520816A priority patent/JP2006528482A/ja
Priority to CA002533119A priority patent/CA2533119A1/en
Publication of WO2005010209A2 publication Critical patent/WO2005010209A2/de
Publication of WO2005010209A3 publication Critical patent/WO2005010209A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur reversen Transkription und/oder Amplifikation eines Produktes aus einer reversen Transkription eines Pools von Nukleinsäuren eines bestimmten Typs, wobei dieser Pool von Nukleinsäuren aus einer komplexen biologischen Probe oder einer enzymatischen Reaktion stammt.
PCT/EP2004/008363 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren WO2005010209A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2004259851A AU2004259851A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
EP04763506A EP1651776A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
US10/565,989 US20060281092A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
JP2006520816A JP2006528482A (ja) 2003-07-24 2004-07-26 核酸の逆転写及び／または増幅方法
CA002533119A CA2533119A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48964303P 2003-07-24 2003-07-24
US60/489,643 2003-07-24

Publications (2)

Publication Number Publication Date
WO2005010209A2 WO2005010209A2 (de) 2005-02-03
WO2005010209A3 true WO2005010209A3 (de) 2005-05-26

Family

ID=34102910

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/008363 WO2005010209A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren

Country Status (6)

Country Link
US (1) US20060281092A1 (de)
EP (1) EP1651776A2 (de)
JP (1) JP2006528482A (de)
AU (1) AU2004259851A1 (de)
CA (1) CA2533119A1 (de)
WO (1) WO2005010209A2 (de)

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US20060234277A1 (en) * 2005-03-31 2006-10-19 Amgen Inc. Method for selectively blocking hemoglobin RNA amplification
BRPI0605857A (pt) 2005-04-19 2007-12-18 Coding Tech Ab quantização dependente de energia para a codificação eficiente de parámetros de áudio espacial
EP2479291A1 (de) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleinsäureverstärkung unter Verwendung nichtzufälliger Primer
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
CA2773887A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
GB2504240B (en) 2012-02-27 2015-05-27 Cellular Res Inc Compositions and kits for molecular counting of nucleic acids
WO2013158215A1 (en) * 2012-04-16 2013-10-24 Life Technologies Corporation Oligonucleotides and methods for the preparationof rna libraries
JP6545682B2 (ja) 2013-08-28 2019-07-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 大規模並列単一細胞分析
EP3262192B1 (de) 2015-02-27 2020-09-16 Becton, Dickinson and Company Räumlich adressierbare molekulare barcodierung
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
EP3303587A4 (de) * 2015-05-28 2018-11-21 Kaarel Krjutskov Blockierung von oligonukleotiden
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CN110506114B (zh) * 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
CN112243461B (zh) 2018-05-03 2024-07-12 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP2022502028A (ja) * 2018-09-25 2022-01-11 キアゲン サイエンシーズ， エルエルシー 望ましくないｒｎａ種の消去
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) * 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
EP4004231A1 (de) 2019-07-22 2022-06-01 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins

Citations (5)

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US5891625A (en) * 1992-06-05 1999-04-06 Buchardt Ole Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001060517A2 (de) * 2000-02-15 2001-08-23 Antigene Biotech Gmbh Gefäss zur nukleinsäureanalytik
WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

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US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
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Publication number Priority date Publication date Assignee Title
US5891625A (en) * 1992-06-05 1999-04-06 Buchardt Ole Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001060517A2 (de) * 2000-02-15 2001-08-23 Antigene Biotech Gmbh Gefäss zur nukleinsäureanalytik
WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

Non-Patent Citations (4)

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HOMANN M ET AL: "INCORPORATION OF THE CATALYTIC DOMAIN OF A HAMMERHEAD RIBOZYME INTOANTISENSE RNA ENHANCES ITS INHIBITORY EFFECT ON THE REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 12, 1993, pages 2809 - 2814, XP002051227, ISSN: 0305-1048 *
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Also Published As

Publication number Publication date
US20060281092A1 (en) 2006-12-14
AU2004259851A1 (en) 2005-02-03
WO2005010209A2 (de) 2005-02-03
JP2006528482A (ja) 2006-12-21
EP1651776A2 (de) 2006-05-03
CA2533119A1 (en) 2005-02-03

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