WO2007014544A3 - Stereoselektive synthese von chiralen diolen - Google Patents

Stereoselektive synthese von chiralen diolen Download PDF

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Publication number
WO2007014544A3
WO2007014544A3 PCT/DE2006/001247 DE2006001247W WO2007014544A3 WO 2007014544 A3 WO2007014544 A3 WO 2007014544A3 DE 2006001247 W DE2006001247 W DE 2006001247W WO 2007014544 A3 WO2007014544 A3 WO 2007014544A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
chiral
stereoselective synthesis
synthesis
chiral diols
Prior art date
Application number
PCT/DE2006/001247
Other languages
English (en)
French (fr)
Other versions
WO2007014544A2 (de
Inventor
Thomas Daussmann
Original Assignee
Julich Chiral Solutions Gmbh
Thomas Daussmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Julich Chiral Solutions Gmbh, Thomas Daussmann filed Critical Julich Chiral Solutions Gmbh
Publication of WO2007014544A2 publication Critical patent/WO2007014544A2/de
Publication of WO2007014544A3 publication Critical patent/WO2007014544A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur stereoselektiven Synthese von chiralen Diolen, chiralen Hydroxyketonen, chiralen Alkoholen und deren Derivate, bei dem als Substrat ein Dion, ein prochirales Keton oder ein Hydroxyketon in eine Kultur ganzer Zellen gegeben wird, welche ein Enzym produzieren, das als Katalysator für die Umsetzung des Substrats dient. Erfindungsgemäß wird zunächst in Enzymnegative Zellen mindestens eine rekombinante Nukleinsäure eingebracht, die zumindest das Gen für das Enzym umfasst, und anschließend werden die derart hergestellten Enzym-positiven Zellen für die Synthese eingesetzt.
PCT/DE2006/001247 2005-08-02 2006-07-16 Stereoselektive synthese von chiralen diolen WO2007014544A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005036880.8 2005-08-02
DE102005036880A DE102005036880A1 (de) 2005-08-02 2005-08-02 Stereoselektive Synthese von chiralen Diolen

Publications (2)

Publication Number Publication Date
WO2007014544A2 WO2007014544A2 (de) 2007-02-08
WO2007014544A3 true WO2007014544A3 (de) 2007-08-02

Family

ID=37651023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2006/001247 WO2007014544A2 (de) 2005-08-02 2006-07-16 Stereoselektive synthese von chiralen diolen

Country Status (2)

Country Link
DE (1) DE102005036880A1 (de)
WO (1) WO2007014544A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006055047A1 (de) * 2006-11-22 2008-05-29 Wacker Chemie Ag Verfahren zur Herstellung von Dihydroxy-Verbindungen aus Diketo-Verbindungen durch enzymkatalysierte enantioselektive Reduktion
DE102008064575B4 (de) * 2008-12-31 2011-06-01 Barakat, Adil Verfahren zur Herstellung von 1-Hydroxyketon-Verbindungen
DE102011003386A1 (de) * 2011-01-31 2012-08-02 Wacker Chemie Ag Verfahren zur fermentativen Herstellung von 2,3-Butandiol
EP2729576A2 (de) * 2011-07-07 2014-05-14 Novozymes A/S Enzymatische zubereitung von diolen
CN104560745B (zh) * 2014-12-08 2017-12-01 上海应用技术学院 一种毕赤酵母菌Pichia sp.SIT2014及其培养方法和应用
CN110643556A (zh) * 2019-08-23 2020-01-03 浙江工业大学 一种共表达烯醛还原酶和葡萄糖脱氢酶的重组基因工程菌及其应用
CN114958927A (zh) * 2021-02-22 2022-08-30 尚科生物医药（上海）有限公司 一种制备(s)-1-(3-氯苯基)-1,3-丙二醇的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1067195A2 (de) * 1999-07-09 2001-01-10 Forschungszentrum Jülich Gmbh Verfahren zur Reduktion von Ketogruppen enthaltende Verbindungen
WO2004013332A1 (en) * 2002-08-01 2004-02-12 Nederlands Instituut Voor Zuivelonderzoek Substrate conversion
WO2005045016A2 (en) * 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10218689A1 (de) * 2002-04-26 2003-11-20 Degussa ADH aus Rhodococcus erythropolis
DE10300335B4 (de) * 2003-01-09 2008-06-26 Iep Gmbh Oxidoreduktase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1067195A2 (de) * 1999-07-09 2001-01-10 Forschungszentrum Jülich Gmbh Verfahren zur Reduktion von Ketogruppen enthaltende Verbindungen
WO2004013332A1 (en) * 2002-08-01 2004-02-12 Nederlands Instituut Voor Zuivelonderzoek Substrate conversion
WO2005045016A2 (en) * 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EM_PRO 1 April 2004 (2004-04-01), SCHNEIDER F ET AL: "Lactobacillus kefiri DSM 20587 NADPH dependent R-specific alcohol dehydrogenase (adhR) gene, complete cds.", XP002416806, Database accession no. AY267012 *
DATABASE EM_PRO 17 February 2003 (2003-02-17), RIEBEL-BOMMARIUS B R: "Lactobacillus brevis radh gene for R-specific alcohol dehydrogenase", XP002416807, Database accession no. AJ544275 *
ERNST M ET AL: "Enantioselective reduction of carbonyl compounds by whole-cell biotransformation, combining a formate dehydrogenase and a (R)-specific alcohol dehydrogenase", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 66, November 2004 (2004-11-01), pages 629 - 634, XP002344978, ISSN: 0175-7598 *
KATAOKA M ET AL: "NOVEL BIOREDUCTION SYSTEM FOR THE PRODUCTION OF CHIRAL ALCOHOLS", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 62, no. 5-6, October 2003 (2003-10-01), pages 437 - 445, XP009049508, ISSN: 0175-7598 *
MATSUYAMA A ET AL: "Practical application of recombinant whole-cell biocatalysts for the manufacturing of pharmaceutical intermediates such as chiral alcohols", ORGANIC PROCESS RESEARCH AND DEVELOPMENT, CAMBRIDGE, GB, vol. 6, 2002, pages 558 - 561, XP002344756 *
WECKBECKER A ET AL: "Improved synthesis of chiral alcohols with Escherichia coli cells co-expressing pyridine nucleotide transhydrogenase, NADP(+)-dependent alcohol dehydrogenase and NAD(+)-dependent formate dehydrogenase", BIOTECHNOLOGY LETTERS, KEW, SURREY, GB, vol. 26, November 2004 (2004-11-01), pages 1739 - 1744, XP002344979, ISSN: 0141-5492 *

Also Published As

Publication number Publication date
DE102005036880A1 (de) 2007-02-08
WO2007014544A2 (de) 2007-02-08

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