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Method for small volume nucleic acid synthesis

Info

Publication number
WO2005030984A3
WO2005030984A3 PCT/US2004/031804 US2004031804W WO2005030984A3 WO 2005030984 A3 WO2005030984 A3 WO 2005030984A3 US 2004031804 W US2004031804 W US 2004031804W WO 2005030984 A3 WO2005030984 A3 WO 2005030984A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
synthesis
cdna
method
nucleic
volume
Prior art date
Application number
PCT/US2004/031804
Other languages
French (fr)
Other versions
WO2005030984A2 (en )
Inventor
James M Kadushin
Original Assignee
Datascope Investment Corp
James M Kadushin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Nucleic acid analysis involving immobilisation; Immobilisation characterised by the carrier or coupling agent
    • C12Q1/6837Nucleic acid analysis involving immobilisation; Immobilisation characterised by the carrier or coupling agent characterised by the use of probe arrays or probe chips

Abstract

A method for synthesis of nucleic acids, with particular use for synthesis of cDNA. The method allows application of the cDNA to a microarray without the need for concentration or purification of the cDNA post-cDNA synthesis.
PCT/US2004/031804 2003-09-26 2004-09-27 Method for small volume nucleic acid synthesis WO2005030984A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US50624703 true 2003-09-26 2003-09-26
US60/506,247 2003-09-26

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20040789156 EP1664350A4 (en) 2003-09-26 2004-09-27 Method for small volume nucleic acid synthesis
JP2006528313A JP2007506439A (en) 2003-09-26 2004-09-27 Method for synthesizing a small amount of nucleic acid
US11584751 US20080033159A1 (en) 2001-01-13 2006-10-20 Method for small volume nucleic acid synthesis

Publications (2)

Publication Number Publication Date
WO2005030984A2 true WO2005030984A2 (en) 2005-04-07
WO2005030984A3 true true WO2005030984A3 (en) 2005-12-22

Family

ID=34393128

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/031804 WO2005030984A3 (en) 2003-09-26 2004-09-27 Method for small volume nucleic acid synthesis

Country Status (3)

Country Link
JP (1) JP2007506439A (en)
EP (1) EP1664350A4 (en)
WO (1) WO2005030984A3 (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
CA2833262A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487973A (en) * 1986-09-10 1996-01-30 Polyprobe, Inc. Methods for detecting and assaying nucleic acid sequences
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2400680A1 (en) * 2000-03-08 2001-09-13 Datascope Investment Corp. Methods for assay and detection on a microarray

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5487973A (en) * 1986-09-10 1996-01-30 Polyprobe, Inc. Methods for detecting and assaying nucleic acid sequences
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1664350A2 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9480983B2 (en) 2011-09-30 2016-11-01 Becton, Dickinson And Company Unitized reagent strip
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip

Also Published As

Publication number Publication date Type
EP1664350A4 (en) 2007-10-10 application
WO2005030984A2 (en) 2005-04-07 application
JP2007506439A (en) 2007-03-22 application
EP1664350A2 (en) 2006-06-07 application

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