BR112012019396A2 - método e kit para amplificar um ácido nucleico. - Google Patents

método e kit para amplificar um ácido nucleico.

Info

Publication number
BR112012019396A2
BR112012019396A2 BR112012019396A BR112012019396A BR112012019396A2 BR 112012019396 A2 BR112012019396 A2 BR 112012019396A2 BR 112012019396 A BR112012019396 A BR 112012019396A BR 112012019396 A BR112012019396 A BR 112012019396A BR 112012019396 A2 BR112012019396 A2 BR 112012019396A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
amplifying
kit
methods
kits
Prior art date
Application number
BR112012019396A
Other languages
English (en)
Inventor
Richard Nelson John
Original Assignee
General Eletric Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Eletric Company filed Critical General Eletric Company
Publication of BR112012019396A2 publication Critical patent/BR112012019396A2/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

método e kit para amplificar um ácido nucleico. métodos e kits para amplificar um ácido nucleico sob condições isotérmicas para formar uma sequência de ácido nucleico amplificada são fornecidos. os métodos e kits compreendem fornecer um modelo de ácido nucleico, uma dna polimerase, trifosfatos de desoxirribonucleosídeo, um inibidor que compreende uma sequência aleatória e um iniciador específico e amplificar o modelo de ácido nucleico.
BR112012019396A 2010-02-09 2011-02-08 método e kit para amplificar um ácido nucleico. BR112012019396A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/702,884 US20110195457A1 (en) 2010-02-09 2010-02-09 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
PCT/US2011/023996 WO2011142861A2 (en) 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof

Publications (1)

Publication Number Publication Date
BR112012019396A2 true BR112012019396A2 (pt) 2017-06-06

Family

ID=44354018

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012019396A BR112012019396A2 (pt) 2010-02-09 2011-02-08 método e kit para amplificar um ácido nucleico.

Country Status (9)

Country Link
US (1) US20110195457A1 (pt)
EP (1) EP2534268A4 (pt)
JP (1) JP2013518598A (pt)
CN (1) CN102753707A (pt)
AU (1) AU2011253427B2 (pt)
BR (1) BR112012019396A2 (pt)
CA (1) CA2788821A1 (pt)
IN (1) IN2012DN06299A (pt)
WO (1) WO2011142861A2 (pt)

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US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
ES2917400T3 (es) * 2012-07-26 2022-07-08 Illumina Inc Composiciones y métodos para la amplificación de ácidos nucleicos
JP6366896B2 (ja) * 2013-03-07 2018-08-01 国立研究開発法人農業・食品産業技術総合研究機構 Dna親和性物質含有組成物、dna親和性物質の精製方法、dna親和性物質含有組成物の製造方法およびdna親和性物質を精製するためのキット
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9644232B2 (en) * 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9938568B2 (en) 2013-07-26 2018-04-10 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
KR101670232B1 (ko) * 2013-10-28 2016-10-31 한국과학기술연구원 다공성 구조체 및 이의 제조방법
US10428376B2 (en) * 2013-12-02 2019-10-01 Regents Of The University Of Minnesota RNA amplification and oligonucleotide library preparation
US9587263B2 (en) 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
US10704080B2 (en) 2014-06-11 2020-07-07 Samplix Aps Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS)
US11085073B2 (en) 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
EP3371326B1 (en) * 2015-11-05 2023-11-01 Global Life Sciences Solutions Operations UK Ltd Ligase-assisted nucleic acid circularization and amplification
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたdnaライブラリーの作製方法
US11760995B2 (en) * 2017-06-20 2023-09-19 Mgi Tech Co., Ltd. PCR primer pair and application thereof
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒
CN110195056A (zh) * 2019-05-31 2019-09-03 汪雪 一种双引物及其应用
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches

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DE69934088T2 (de) * 1998-01-09 2007-06-21 University Of Utah Research Foundation, Salt Lake City Verfahren zur in vitro amplifikation zirkulärer dna
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US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
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AU2004254552B2 (en) * 2003-01-29 2008-04-24 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
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BRPI0607661A2 (pt) * 2005-04-29 2009-09-22 J Craig Venter Inst amplificação e clonagem de molécula de único dna usando amplificação de cìrculo rolante
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Also Published As

Publication number Publication date
CN102753707A (zh) 2012-10-24
WO2011142861A3 (en) 2012-02-02
US20110195457A1 (en) 2011-08-11
WO2011142861A2 (en) 2011-11-17
CA2788821A1 (en) 2011-11-17
EP2534268A2 (en) 2012-12-19
AU2011253427A1 (en) 2012-08-23
JP2013518598A (ja) 2013-05-23
WO2011142861A9 (en) 2012-03-15
AU2011253427B2 (en) 2015-06-25
IN2012DN06299A (pt) 2015-09-25
EP2534268A4 (en) 2013-09-04

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]