AR088998A1 - Ensayo de alto rendimiento de polimorfismo de nucleotido simple - Google Patents
Ensayo de alto rendimiento de polimorfismo de nucleotido simpleInfo
- Publication number
- AR088998A1 AR088998A1 ARP120104448A ARP120104448A AR088998A1 AR 088998 A1 AR088998 A1 AR 088998A1 AR P120104448 A ARP120104448 A AR P120104448A AR P120104448 A ARP120104448 A AR P120104448A AR 088998 A1 AR088998 A1 AR 088998A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleotide polymorphism
- high performance
- performance test
- simple nucleotide
- cygosity
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se provee un método que consiste en un sistema de detección de ensayo homogéneo para un proceso de PCR que utiliza FRET para detección y análisis de cigosidad del polimorfismo de nucleótido simple en girasol. El método provee cebadores de genoma de girasol específicos que pueden utilizarse para detectar la presencia o ausencia del polimorfismo de nucleótido simple HaAHASL1-A122(At)T. Se describen las combinaciones de cebador para uso en un ensayo PCR de punto final capaz de determinar la cigosidad y para ayudar a promover la introgresión.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161564464P | 2011-11-29 | 2011-11-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR088998A1 true AR088998A1 (es) | 2014-07-23 |
Family
ID=48467207
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120104448A AR088998A1 (es) | 2011-11-29 | 2012-11-27 | Ensayo de alto rendimiento de polimorfismo de nucleotido simple |
Country Status (10)
Country | Link |
---|---|
US (1) | US20130137097A1 (es) |
EP (1) | EP2786146A4 (es) |
CN (1) | CN103988079A (es) |
AR (1) | AR088998A1 (es) |
BR (1) | BR112014012773A2 (es) |
CA (1) | CA2854790A1 (es) |
CL (1) | CL2014001406A1 (es) |
RU (1) | RU2014126423A (es) |
UY (1) | UY34473A (es) |
WO (1) | WO2013081987A1 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
JP2022525322A (ja) | 2019-03-14 | 2022-05-12 | インシリクサ, インコーポレイテッド | 時間ゲート蛍光ベースの検出のための方法およびシステム |
CN116287373B (zh) * | 2022-12-31 | 2024-07-09 | 中国农业大学 | 小麦穗粒数主效qtl紧密连锁的kasp分子标记及其方法与应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110014706A2 (en) * | 1998-12-14 | 2011-01-20 | Monsanto Technology Llc | Arabidopsis thaliana Genome Sequence and Uses Thereof |
JP2003325200A (ja) * | 2002-03-08 | 2003-11-18 | Kazuko Matsumoto | 新規高感度核酸解析法 |
EP1902136B1 (en) * | 2005-07-01 | 2012-01-18 | Basf Se | Herbicide-resistant sunflower plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use |
EP1956097A1 (en) * | 2007-02-06 | 2008-08-13 | bioMerieux B.V. | Method for discriminating single nucleotide polymorphisms (SNPs) |
JP5715414B2 (ja) * | 2007-04-04 | 2015-05-07 | ビーエーエスエフ アグロケミカル プロダクツ ビー.ブイ. | Ahasl1の複数の除草剤耐性対立遺伝子を有する除草剤耐性ヒマワリ植物及び使用方法 |
EA036108B1 (ru) * | 2007-04-04 | 2020-09-29 | Басф Се | Резистентные к гербицидам растения brassica и способы применения |
GB0719775D0 (en) * | 2007-10-10 | 2007-11-21 | Isis Innovation | Extended haplotypes |
CN101220395A (zh) * | 2008-02-03 | 2008-07-16 | 中国科学院化学研究所 | 一种检测dna单核苷酸多态性的方法 |
US20120060243A1 (en) * | 2008-09-26 | 2012-03-08 | Peter Beetham | Herbicide-Resistant AHAS-Mutants and Methods of Use |
CN102191314A (zh) * | 2010-03-12 | 2011-09-21 | 孙朝辉 | 一种基于pcr的低成本、简单、高效的snp检测方法 |
-
2012
- 2012-11-27 CA CA2854790A patent/CA2854790A1/en not_active Abandoned
- 2012-11-27 WO PCT/US2012/066577 patent/WO2013081987A1/en active Application Filing
- 2012-11-27 AR ARP120104448A patent/AR088998A1/es unknown
- 2012-11-27 RU RU2014126423A patent/RU2014126423A/ru not_active Application Discontinuation
- 2012-11-27 CN CN201280058913.8A patent/CN103988079A/zh active Pending
- 2012-11-27 US US13/685,903 patent/US20130137097A1/en not_active Abandoned
- 2012-11-27 BR BR112014012773A patent/BR112014012773A2/pt not_active IP Right Cessation
- 2012-11-27 EP EP12854185.1A patent/EP2786146A4/en not_active Withdrawn
- 2012-11-28 UY UY0001034473A patent/UY34473A/es not_active Application Discontinuation
-
2014
- 2014-05-28 CL CL2014001406A patent/CL2014001406A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
WO2013081987A1 (en) | 2013-06-06 |
CL2014001406A1 (es) | 2015-01-16 |
BR112014012773A2 (pt) | 2019-09-24 |
EP2786146A4 (en) | 2015-08-26 |
US20130137097A1 (en) | 2013-05-30 |
UY34473A (es) | 2013-06-28 |
RU2014126423A (ru) | 2016-01-27 |
CA2854790A1 (en) | 2013-06-06 |
CN103988079A (zh) | 2014-08-13 |
EP2786146A1 (en) | 2014-10-08 |
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