AR088998A1 - Ensayo de alto rendimiento de polimorfismo de nucleotido simple - Google Patents

Ensayo de alto rendimiento de polimorfismo de nucleotido simple

Info

Publication number
AR088998A1
AR088998A1 ARP120104448A ARP120104448A AR088998A1 AR 088998 A1 AR088998 A1 AR 088998A1 AR P120104448 A ARP120104448 A AR P120104448A AR P120104448 A ARP120104448 A AR P120104448A AR 088998 A1 AR088998 A1 AR 088998A1
Authority
AR
Argentina
Prior art keywords
nucleotide polymorphism
high performance
performance test
simple nucleotide
cygosity
Prior art date
Application number
ARP120104448A
Other languages
English (en)
Original Assignee
Agrigenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agrigenetics Inc filed Critical Agrigenetics Inc
Publication of AR088998A1 publication Critical patent/AR088998A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se provee un método que consiste en un sistema de detección de ensayo homogéneo para un proceso de PCR que utiliza FRET para detección y análisis de cigosidad del polimorfismo de nucleótido simple en girasol. El método provee cebadores de genoma de girasol específicos que pueden utilizarse para detectar la presencia o ausencia del polimorfismo de nucleótido simple HaAHASL1-A122(At)T. Se describen las combinaciones de cebador para uso en un ensayo PCR de punto final capaz de determinar la cigosidad y para ayudar a promover la introgresión.
ARP120104448A 2011-11-29 2012-11-27 Ensayo de alto rendimiento de polimorfismo de nucleotido simple AR088998A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161564464P 2011-11-29 2011-11-29

Publications (1)

Publication Number Publication Date
AR088998A1 true AR088998A1 (es) 2014-07-23

Family

ID=48467207

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120104448A AR088998A1 (es) 2011-11-29 2012-11-27 Ensayo de alto rendimiento de polimorfismo de nucleotido simple

Country Status (10)

Country Link
US (1) US20130137097A1 (es)
EP (1) EP2786146A4 (es)
CN (1) CN103988079A (es)
AR (1) AR088998A1 (es)
BR (1) BR112014012773A2 (es)
CA (1) CA2854790A1 (es)
CL (1) CL2014001406A1 (es)
RU (1) RU2014126423A (es)
UY (1) UY34473A (es)
WO (1) WO2013081987A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110014706A2 (en) * 1998-12-14 2011-01-20 Monsanto Technology Llc Arabidopsis thaliana Genome Sequence and Uses Thereof
JP2003325200A (ja) * 2002-03-08 2003-11-18 Kazuko Matsumoto 新規高感度核酸解析法
PT1902136E (pt) * 2005-07-01 2012-04-13 Basf Se Plantas de girassol resistentes a herbicidas, polinucleótidos que codificam para proteínas de subunidades grandes de aceto-hidroxiácido sintase resistentes a herbicidas e métodos de uso
EP1956097A1 (en) * 2007-02-06 2008-08-13 bioMerieux B.V. Method for discriminating single nucleotide polymorphisms (SNPs)
EP2392661B1 (en) * 2007-04-04 2016-11-09 Basf Se Herbicide-resistant Brassica plants and methods of use
CN102808017B (zh) * 2007-04-04 2016-01-13 巴斯夫农业化学品有限公司 具有多个除草剂抗性ahasl1等位基因的除草剂抗性向日葵植物及使用方法
GB0719775D0 (en) * 2007-10-10 2007-11-21 Isis Innovation Extended haplotypes
CN101220395A (zh) * 2008-02-03 2008-07-16 中国科学院化学研究所 一种检测dna单核苷酸多态性的方法
EP2733212B1 (en) * 2008-09-26 2018-09-05 BASF Agrochemical Products, B.V. Herbicide-resistant ahas-mutants and methods of use
CN102191314A (zh) * 2010-03-12 2011-09-21 孙朝辉 一种基于pcr的低成本、简单、高效的snp检测方法

Also Published As

Publication number Publication date
RU2014126423A (ru) 2016-01-27
EP2786146A4 (en) 2015-08-26
WO2013081987A1 (en) 2013-06-06
UY34473A (es) 2013-06-28
CN103988079A (zh) 2014-08-13
US20130137097A1 (en) 2013-05-30
CL2014001406A1 (es) 2015-01-16
BR112014012773A2 (pt) 2019-09-24
EP2786146A1 (en) 2014-10-08
CA2854790A1 (en) 2013-06-06

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