SG10201807867XA - Simultaneous detection of target protein and target nucleic acids in a single cell - Google Patents

Simultaneous detection of target protein and target nucleic acids in a single cell

Info

Publication number
SG10201807867XA
SG10201807867XA SG10201807867XA SG10201807867XA SG10201807867XA SG 10201807867X A SG10201807867X A SG 10201807867XA SG 10201807867X A SG10201807867X A SG 10201807867XA SG 10201807867X A SG10201807867X A SG 10201807867XA SG 10201807867X A SG10201807867X A SG 10201807867XA
Authority
SG
Singapore
Prior art keywords
nucleic acids
single cell
target
simultaneous detection
target nucleic
Prior art date
Application number
SG10201807867XA
Inventor
Robert C Jones
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of SG10201807867XA publication Critical patent/SG10201807867XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The kits, reagents, and methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids ( e.g., RNA) and proteins. FIG. 1
SG10201807867XA 2013-03-15 2014-03-14 Simultaneous detection of target protein and target nucleic acids in a single cell SG10201807867XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361799559P 2013-03-15 2013-03-15

Publications (1)

Publication Number Publication Date
SG10201807867XA true SG10201807867XA (en) 2018-10-30

Family

ID=51537694

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201504877XA SG11201504877XA (en) 2013-03-15 2014-03-14 Simultaneous detection of target protein and target nucleic acids in a single cell
SG10201807867XA SG10201807867XA (en) 2013-03-15 2014-03-14 Simultaneous detection of target protein and target nucleic acids in a single cell

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201504877XA SG11201504877XA (en) 2013-03-15 2014-03-14 Simultaneous detection of target protein and target nucleic acids in a single cell

Country Status (8)

Country Link
US (3) US9809848B2 (en)
EP (1) EP2971121A4 (en)
JP (1) JP2016514455A (en)
CN (1) CN105121659A (en)
CA (1) CA2895605A1 (en)
RU (1) RU2015123459A (en)
SG (2) SG11201504877XA (en)
WO (1) WO2014144371A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105121659A (en) * 2013-03-15 2015-12-02 富鲁达公司 Simultaneous detection of target protein and target nucleic acids in a single cell
CA2922926A1 (en) * 2013-09-04 2015-03-12 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
WO2016123419A1 (en) * 2015-01-30 2016-08-04 President And Fellows Of Harvard College Microscope-free imaging
US11981956B2 (en) 2018-01-26 2024-05-14 President And Fellows Of Harvard College Proximity detection methods and compositions
EP3950955A1 (en) 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyte assay for the simultaneous detection of nucleic acid and analytes

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0666927T3 (en) 1991-09-13 1997-09-15 Cytocell Ltd A method for detecting a nucleic acid sequence.
CA2319662C (en) * 1998-01-27 2009-11-24 Cytocell Limited Modified nucleic acid probes and uses thereof
DE60026641T2 (en) 1999-07-15 2007-01-25 Qiagen Gmbh Method of separating particulate substrates from solution by minimizing particle loss
EP1334347A1 (en) 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
PT1842226E (en) 2004-11-03 2010-11-29 Iris Molecular Diagnostics Inc Homogeneous analyte detection
EP2484781B1 (en) 2006-08-01 2017-08-23 Applied Biosystems, LLC Detection of analytes and nucleic acids
US7972828B2 (en) 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
CN101382552A (en) 2007-09-05 2009-03-11 周国华 Method for detecting protein content difference
BRPI1015218B1 (en) 2009-05-07 2019-10-08 Biomerieux Inc. METHODS FOR DETERMINING ANTIMICROBIAN RESISTANCE
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
US10597701B2 (en) * 2011-05-11 2020-03-24 Navinci Diagnostics Ab Unfolding proximity probes and methods for the use thereof
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
CN105121659A (en) * 2013-03-15 2015-12-02 富鲁达公司 Simultaneous detection of target protein and target nucleic acids in a single cell
CA2922926A1 (en) 2013-09-04 2015-03-12 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell

Also Published As

Publication number Publication date
EP2971121A4 (en) 2016-11-30
EP2971121A1 (en) 2016-01-20
CN105121659A (en) 2015-12-02
WO2014144371A1 (en) 2014-09-18
SG11201504877XA (en) 2015-07-30
US20190352697A1 (en) 2019-11-21
RU2015123459A (en) 2017-04-20
CA2895605A1 (en) 2014-09-18
US20160024557A1 (en) 2016-01-28
US9809848B2 (en) 2017-11-07
US20180087092A1 (en) 2018-03-29
JP2016514455A (en) 2016-05-23
US10214773B2 (en) 2019-02-26

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