BR112014020422A2 - método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra, oligonucleotídeo iniciador isolado ou partzime, duplex de ácido nucleico de cadeia dupla isolado, enzima de ácido nucleico multi-componente, complexo de ácido nucleico, uso da mnazyme e kit - Google Patents
método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra, oligonucleotídeo iniciador isolado ou partzime, duplex de ácido nucleico de cadeia dupla isolado, enzima de ácido nucleico multi-componente, complexo de ácido nucleico, uso da mnazyme e kitInfo
- Publication number
- BR112014020422A2 BR112014020422A2 BR112014020422-5A BR112014020422A BR112014020422A2 BR 112014020422 A2 BR112014020422 A2 BR 112014020422A2 BR 112014020422 A BR112014020422 A BR 112014020422A BR 112014020422 A2 BR112014020422 A2 BR 112014020422A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- isolated
- partzime
- mnazyme
- kit
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2012900624A AU2012900624A0 (en) | 2012-02-20 | Detection of Nucleic Acids | |
| AU2012900624 | 2012-02-20 | ||
| AU2012902218 | 2012-05-29 | ||
| AU2012902218A AU2012902218A0 (en) | 2012-05-29 | Detection of nucleic acids | |
| PCT/AU2013/000149 WO2013123552A1 (en) | 2012-02-20 | 2013-02-20 | Detection of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112014020422A2 true BR112014020422A2 (pt) | 2018-10-30 |
| BR112014020422B1 BR112014020422B1 (pt) | 2021-12-28 |
Family
ID=49004851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014020422-5A BR112014020422B1 (pt) | 2012-02-20 | 2013-02-20 | Método para determinar a presença ou ausência de um polinucleotídeo alvo em uma amostra |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US9963738B2 (pt) |
| EP (1) | EP2817421B1 (pt) |
| JP (2) | JP6276201B2 (pt) |
| KR (1) | KR102085119B1 (pt) |
| CN (1) | CN104245958B (pt) |
| AU (1) | AU2013202920B9 (pt) |
| BR (1) | BR112014020422B1 (pt) |
| CA (1) | CA2863808C (pt) |
| DK (1) | DK2817421T3 (pt) |
| ES (1) | ES2660248T3 (pt) |
| HK (1) | HK1199743A1 (pt) |
| IL (1) | IL233958A (pt) |
| MX (1) | MX353840B (pt) |
| NO (1) | NO2817421T3 (pt) |
| NZ (1) | NZ628883A (pt) |
| SG (1) | SG11201405012YA (pt) |
| WO (1) | WO2013123552A1 (pt) |
| ZA (1) | ZA201406410B (pt) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11201400464QA (en) | 2011-09-09 | 2014-06-27 | Speedx Pty Ltd | Nucleic acid enzyme substrates |
| WO2013123552A1 (en) | 2012-02-20 | 2013-08-29 | Speedx Pty Ltd | Detection of nucleic acids |
| AU2013202354A1 (en) * | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| AU2014214725B2 (en) | 2013-02-07 | 2018-04-05 | Rutgers, The State University Of New Jersey | Highly selective nucleic acid amplification primers |
| CN103540660B (zh) * | 2013-10-10 | 2015-10-28 | 中国人民解放军疾病预防控制所 | 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒 |
| WO2015162438A1 (en) * | 2014-04-25 | 2015-10-29 | Dna Electronics Ltd | Sequencing methods |
| KR101631114B1 (ko) * | 2014-06-13 | 2016-06-16 | 서울대학교산학협력단 | 육종감별을 위한 등온증폭반응용 프라이머 세트 및 이를 이용한 육종 감별 방법 |
| WO2016077291A1 (en) * | 2014-11-10 | 2016-05-19 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isothermal amplification assay for the detection of short nucleic acid sequences |
| US20180163261A1 (en) * | 2015-02-26 | 2018-06-14 | Asuragen, Inc. | Methods and apparatuses for improving mutation assessment accuracy |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| CN108026569B (zh) * | 2015-07-17 | 2021-08-24 | 卢米耐克斯公司 | 用于催化性测定的方法和组合物 |
| WO2019030383A1 (en) | 2017-08-10 | 2019-02-14 | Katholieke Universiteit Leuven | ENZYME SENSOR OF NUCLEIC ACIDS |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| KR101987388B1 (ko) * | 2017-10-19 | 2019-06-12 | 경상대학교산학협력단 | 분자비콘을 포함하는 비브리오균의 등온비색 검출용 조성물 및 이의 용도 |
| CN107966438B (zh) * | 2017-10-27 | 2020-11-24 | 中国农业大学 | 一种基于锌的功能核酸的耐高盐传感器及其应用 |
| WO2019150414A1 (ja) * | 2018-01-30 | 2019-08-08 | 学校法人 慶應義塾 | 核酸の検出方法 |
| KR20210057028A (ko) * | 2018-08-09 | 2021-05-20 | 스피디엑스 피티와이 리미티드 | 핵산의 다중 검출 |
| CN109097468A (zh) * | 2018-08-14 | 2018-12-28 | 南通市第人民医院 | Opcml基因启动子甲基化水平的检测试剂盒及其应用 |
| CN110452988A (zh) * | 2019-08-27 | 2019-11-15 | 武汉友芝友医疗科技股份有限公司 | 检测esr1基因突变的引物组、试剂、试剂盒和方法 |
| US20220333183A1 (en) | 2019-10-02 | 2022-10-20 | Rutgers, The State University Of New Jersey | Assay methods and kits for detecting rare sequence variants |
| JP2023547498A (ja) | 2020-10-29 | 2023-11-10 | ビオカルティ ナームローゼ フェノーツハップ | マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 |
| CN112662804B (zh) * | 2021-01-25 | 2022-05-10 | 中国水稻研究所 | 一种检测稻瘟病菌无毒基因AvrPi9致病性变异的引物组、试剂盒及方法 |
| EP4288557A1 (en) * | 2021-02-02 | 2023-12-13 | RhoDx, Inc. | Synthetic polynucleotides and methods for selectively amplifying alleles |
| CN112852934B (zh) * | 2021-04-16 | 2022-02-18 | 武汉友芝友医疗科技股份有限公司 | 检测基因甲基化的引物、试剂和试剂盒 |
| GB202405402D0 (en) * | 2021-10-20 | 2024-05-29 | Tataa Biocenter Ab | Methods and compositions for detection of mutant nucleic acid sequences |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121374A1 (en) * | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| KR101243266B1 (ko) * | 2005-03-05 | 2013-03-25 | 주식회사 씨젠 | 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드 |
| DK1948822T3 (da) * | 2005-10-07 | 2011-10-17 | Johnson & Johnson Res Pty Ltd | Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse |
| JP5688507B2 (ja) * | 2006-10-06 | 2015-03-25 | スピーデクス ピーティーワイ リミテッド | 分子スイッチおよびそれらの使用のための方法 |
| ES2425066T3 (es) * | 2007-04-05 | 2013-10-11 | Speedx Pty Ltd | Enzimas y complejos de ácidos nucleicos y métodos para su uso |
| JP2010035532A (ja) * | 2008-08-08 | 2010-02-18 | Canon Inc | アレル特異的ｐｃｒ法 |
| WO2011001496A1 (ja) | 2009-06-29 | 2011-01-06 | 株式会社 東芝 | 検体解析方法およびそれに用いるアッセイキット |
| JP5115825B2 (ja) * | 2009-11-06 | 2013-01-09 | 独立行政法人農業・食品産業技術総合研究機構 | イグサ品種「ひのみどり」の識別マーカー |
| CA2784344C (en) * | 2009-12-21 | 2018-01-02 | Seegene, Inc. | Tsg primer target detection |
| CN102471771B (zh) * | 2010-01-21 | 2016-01-20 | 爱科来株式会社 | 靶序列的扩增方法、多态性检测方法以及其中使用的试剂 |
| WO2013123552A1 (en) | 2012-02-20 | 2013-08-29 | Speedx Pty Ltd | Detection of nucleic acids |
-
2013
- 2013-02-20 WO PCT/AU2013/000149 patent/WO2013123552A1/en active Application Filing
- 2013-02-20 JP JP2014556886A patent/JP6276201B2/ja active Active
- 2013-02-20 KR KR1020147025284A patent/KR102085119B1/ko active IP Right Grant
- 2013-02-20 NO NO13751988A patent/NO2817421T3/no unknown
- 2013-02-20 AU AU2013202920A patent/AU2013202920B9/en active Active
- 2013-02-20 NZ NZ628883A patent/NZ628883A/en unknown
- 2013-02-20 CA CA2863808A patent/CA2863808C/en active Active
- 2013-02-20 BR BR112014020422-5A patent/BR112014020422B1/pt not_active IP Right Cessation
- 2013-02-20 ES ES13751988.0T patent/ES2660248T3/es active Active
- 2013-02-20 EP EP13751988.0A patent/EP2817421B1/en active Active
- 2013-02-20 DK DK13751988.0T patent/DK2817421T3/en active
- 2013-02-20 SG SG11201405012YA patent/SG11201405012YA/en unknown
- 2013-02-20 US US14/379,178 patent/US9963738B2/en active Active
- 2013-02-20 CN CN201380019973.3A patent/CN104245958B/zh active Active
- 2013-02-20 MX MX2014010007A patent/MX353840B/es active IP Right Grant
-
2014
- 2014-08-05 IL IL233958A patent/IL233958A/en active IP Right Grant
- 2014-09-01 ZA ZA2014/06410A patent/ZA201406410B/en unknown
-
2015
- 2015-01-02 HK HK15100017.2A patent/HK1199743A1/xx unknown
-
2018
- 2018-01-11 JP JP2018002618A patent/JP6836302B2/ja active Active
- 2018-03-27 US US15/937,657 patent/US10724080B2/en active Active
-
2020
- 2020-06-25 US US16/912,622 patent/US11802308B2/en active Active
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