CO2018011856A2 - Composiciones, métodos y kits para la síntesis y detección de ácidos nucleicos - Google Patents

Composiciones, métodos y kits para la síntesis y detección de ácidos nucleicos

Info

Publication number
CO2018011856A2
CO2018011856A2 CONC2018/0011856A CO2018011856A CO2018011856A2 CO 2018011856 A2 CO2018011856 A2 CO 2018011856A2 CO 2018011856 A CO2018011856 A CO 2018011856A CO 2018011856 A2 CO2018011856 A2 CO 2018011856A2
Authority
CO
Colombia
Prior art keywords
kits
synthesis
compositions
detection
methods
Prior art date
Application number
CONC2018/0011856A
Other languages
English (en)
Inventor
Ferrier Le
Kalpith Ramamoorthi
Joyce WILDE
Nicole FANTIN
Jordan Lang
David Dupont
Junko Stevens
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of CO2018011856A2 publication Critical patent/CO2018011856A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B01D19/0409—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing Si-atoms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B01D19/0413—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing N-atoms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B01D19/0418—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing P-atoms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B01D19/0422—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing S-atoms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

En la presente se proporcionan composiciones, métodos y kits para sintetizar, detectar y/o cuantificar ácidos nucleicos. Las formas de realización comprenden una composición de amplificación de ácido nucleico que comprende una ADN polimerasa termoestable y agentes que mejoran la síntesis, amplificación, detección y/o cuantificación de ácido nucleico de las dianas de ácido nucleico en un extracto crudo o muestra de lisado crudo.
CONC2018/0011856A 2016-04-06 2018-11-01 Composiciones, métodos y kits para la síntesis y detección de ácidos nucleicos CO2018011856A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662319151P 2016-04-06 2016-04-06
PCT/US2017/026386 WO2017177025A1 (en) 2016-04-06 2017-04-06 Compositions, methods, and kits for synthesis and detection of nucleic acids

Publications (1)

Publication Number Publication Date
CO2018011856A2 true CO2018011856A2 (es) 2019-02-08

Family

ID=58549327

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0011856A CO2018011856A2 (es) 2016-04-06 2018-11-01 Composiciones, métodos y kits para la síntesis y detección de ácidos nucleicos

Country Status (9)

Country Link
US (2) US20170292144A1 (es)
EP (1) EP3440221A1 (es)
JP (3) JP2019515667A (es)
CN (1) CN109072291A (es)
AU (2) AU2017248219B2 (es)
CA (1) CA3017987A1 (es)
CO (1) CO2018011856A2 (es)
RU (1) RU2757416C2 (es)
WO (1) WO2017177025A1 (es)

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CN112574971A (zh) * 2020-12-29 2021-03-30 益善生物技术股份有限公司 Taq DNA聚合酶突变体、PCR反应试剂和试剂盒
CN112725301B (zh) * 2021-03-30 2021-06-25 中国农业科学院生物技术研究所 Taq DNA聚合酶突变体及其应用
WO2023039525A1 (en) * 2021-09-10 2023-03-16 Life Technologies Corporation Master mix compositions, kits, and methods
GB202209241D0 (en) * 2022-06-23 2022-08-10 Biofidelity Ltd Polynucleotide detection
CN115518417A (zh) * 2022-09-01 2022-12-27 天津鸿宇泰生物科技有限公司 用于化学发光检测试剂的消泡剂组合物、使用方法和应用

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Also Published As

Publication number Publication date
AU2017248219A1 (en) 2018-11-01
RU2757416C2 (ru) 2021-10-15
AU2023274253A1 (en) 2023-12-21
RU2018135256A (ru) 2020-05-12
RU2018135256A3 (es) 2021-03-05
JP2023123431A (ja) 2023-09-05
CA3017987A1 (en) 2017-10-12
JP2022084645A (ja) 2022-06-07
US20210277448A1 (en) 2021-09-09
WO2017177025A9 (en) 2018-02-22
AU2017248219B2 (en) 2023-09-07
WO2017177025A1 (en) 2017-10-12
JP2019515667A (ja) 2019-06-13
US20170292144A1 (en) 2017-10-12
CN109072291A (zh) 2018-12-21
EP3440221A1 (en) 2019-02-13

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