SG11201903066RA - Method for multiplex detection of methylated dna - Google Patents

Method for multiplex detection of methylated dna

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Publication number
SG11201903066RA
SG11201903066RA SG11201903066RA SG11201903066RA SG11201903066RA SG 11201903066R A SG11201903066R A SG 11201903066RA SG 11201903066R A SG11201903066R A SG 11201903066RA SG 11201903066R A SG11201903066R A SG 11201903066RA SG 11201903066R A SG11201903066R A SG 11201903066RA
Authority
SG
Singapore
Prior art keywords
target dna
oligonucleotide
target
methylated dna
detecting methylation
Prior art date
Application number
SG11201903066RA
Inventor
Sungwhan An
Taejeong Oh
Original Assignee
Genomictree Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomictree Inc filed Critical Genomictree Inc
Publication of SG11201903066RA publication Critical patent/SG11201903066RA/en

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
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    • C12Q2600/154—Methylation markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Zoology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to a method of detecting methylation of target DNA in a multiplex manner and a composition for detecting methylation of target DNA, and more particularly to a method for detecting methylation of target DNA, comprising: constructing an oligonucleotide, which comprises a target-specific sequence capable of binding complementarily to the target DNA and a universal primer that does not bind complementarily to the target DNA; linearly 10 amplifying the target DNA for linear target enrichment by using the oligonucleotide as a primer; and amplifying the linearly amplified target DNA using an oligonucleotide, which is capable of binding complementarily to the linearly amplified target DNA, a universal primer, and a probe. 15 -50-
SG11201903066RA 2016-10-06 2017-09-29 Method for multiplex detection of methylated dna SG11201903066RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020160129110A KR102006803B1 (en) 2016-10-06 2016-10-06 A Method for Multiple Detection of Methylated DNA
PCT/KR2017/010907 WO2018066910A1 (en) 2016-10-06 2017-09-29 Multiple detection method of methylated dna

Publications (1)

Publication Number Publication Date
SG11201903066RA true SG11201903066RA (en) 2019-05-30

Family

ID=61831748

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201903066RA SG11201903066RA (en) 2016-10-06 2017-09-29 Method for multiplex detection of methylated dna

Country Status (9)

Country Link
US (1) US11186866B2 (en)
EP (1) EP3524688B1 (en)
JP (1) JP6968894B2 (en)
KR (1) KR102006803B1 (en)
CN (1) CN109952381B (en)
AU (1) AU2017339984B2 (en)
ES (1) ES2936408T3 (en)
SG (1) SG11201903066RA (en)
WO (1) WO2018066910A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018256387A1 (en) 2017-04-19 2019-10-31 Singlera Genomics, Inc. Compositions and methods for library construction and sequence analysis
JP7477183B2 (en) * 2019-04-22 2024-05-01 ポステック・リサーチ・アンド・ビジネス・ディベロップメント・ファウンデーション Novel isothermal single reaction probe set and its use
KR102280363B1 (en) * 2019-06-18 2021-07-22 (주)지노믹트리 A Method for Detection of Methylated SDC2 Gene
KR102261606B1 (en) * 2019-11-07 2021-06-07 (주)지노믹트리 Method for Detection of Colorectal Cancer
WO2021097252A1 (en) * 2019-11-13 2021-05-20 Bradley Bernstein Methylation assays and uses thereof
CN111549129B (en) * 2020-03-30 2023-08-29 宁波美康盛德医学检验所有限公司 Kit for detecting gastric cancer and application thereof
CN111653311B (en) * 2020-05-29 2023-05-12 武汉爱基百客生物科技有限公司 Multiplex methylation specific PCR primer design method and system
CN111676286B (en) * 2020-05-29 2023-04-14 武汉爱基百客生物科技有限公司 Multiplex PCR primer system for detecting free DNA methylation of lung cancer plasma, detection method and application
CN114645077A (en) * 2020-12-17 2022-06-21 厦门大学 Method and kit for detecting existence or proportion of donor in receptor sample
CN117980497A (en) * 2021-04-06 2024-05-03 X基因组公司 Systems, methods, and compositions for detecting epigenetic modifications of nucleic acids

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5784146A (en) 1995-12-28 1998-07-21 Nidek Co., Ltd Ophthalmic measurement apparatus
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
WO2007047572A2 (en) * 2005-10-14 2007-04-26 Cleveland State University Methods for identifying multiple dna alteration markers in a large background of wild-type dna
US9090941B2 (en) * 2006-12-19 2015-07-28 Cornell Research Foundation, Inc. Use of lecithin:retinol acyl transferase gene promoter methylation in evaluating the cancer state of subject
KR101255769B1 (en) * 2010-12-16 2013-04-19 (주)지노믹트리 Method for Detecting Methylation of Colorectal Cancer Specific Methylation Marker Gene for Colorectal Cancer Diagnosis
KR101561034B1 (en) * 2014-04-02 2015-10-15 (주)지노믹트리 Method for Methylation Detection of Converted DNA by Bisulfite-Treatment Using Inosine-containing Modified Primers

Also Published As

Publication number Publication date
KR20180038252A (en) 2018-04-16
JP6968894B2 (en) 2021-11-24
AU2017339984B2 (en) 2021-02-04
JP2019536474A (en) 2019-12-19
KR102006803B1 (en) 2019-08-05
EP3524688A1 (en) 2019-08-14
US11186866B2 (en) 2021-11-30
CN109952381B (en) 2022-10-14
EP3524688B1 (en) 2022-12-14
WO2018066910A1 (en) 2018-04-12
AU2017339984A1 (en) 2019-05-23
US20190284608A1 (en) 2019-09-19
EP3524688A4 (en) 2020-05-13
CN109952381A (en) 2019-06-28
ES2936408T3 (en) 2023-03-16

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