ATE175723T1 - Elektrochemische denatusierung von doppelsträngiger nukleinsäure - Google Patents

Elektrochemische denatusierung von doppelsträngiger nukleinsäure

Info

Publication number
ATE175723T1
ATE175723T1 AT95910680T AT95910680T ATE175723T1 AT E175723 T1 ATE175723 T1 AT E175723T1 AT 95910680 T AT95910680 T AT 95910680T AT 95910680 T AT95910680 T AT 95910680T AT E175723 T1 ATE175723 T1 AT E175723T1
Authority
AT
Austria
Prior art keywords
electrochemical
nucleic acid
double stranded
stranded nucleic
denatusation
Prior art date
Application number
AT95910680T
Other languages
English (en)
Inventor
Duncan Ross Purvis
Original Assignee
Scient Generics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9405016A external-priority patent/GB9405016D0/en
Priority claimed from GBGB9502284.4A external-priority patent/GB9502284D0/en
Application filed by Scient Generics Ltd filed Critical Scient Generics Ltd
Application granted granted Critical
Publication of ATE175723T1 publication Critical patent/ATE175723T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
AT95910680T 1994-03-15 1995-03-14 Elektrochemische denatusierung von doppelsträngiger nukleinsäure ATE175723T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9405016A GB9405016D0 (en) 1994-03-15 1994-03-15 Electrical manipulation of nucleic acids
GBGB9502284.4A GB9502284D0 (en) 1995-02-06 1995-02-06 Electrochemical denaturation of double-stranded nucleic acid

Publications (1)

Publication Number Publication Date
ATE175723T1 true ATE175723T1 (de) 1999-01-15

Family

ID=26304504

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95910680T ATE175723T1 (de) 1994-03-15 1995-03-14 Elektrochemische denatusierung von doppelsträngiger nukleinsäure

Country Status (8)

Country Link
US (2) US6033850A (de)
EP (1) EP0750680B1 (de)
JP (1) JPH09510107A (de)
CN (1) CN1059238C (de)
AT (1) ATE175723T1 (de)
DE (1) DE69507289T2 (de)
TW (1) TW533236B (de)
WO (1) WO1995025177A1 (de)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197508B1 (en) 1990-09-12 2001-03-06 Affymetrix, Inc. Electrochemical denaturation and annealing of nucleic acid
CN1059238C (zh) * 1994-03-15 2000-12-06 科学通用有限公司 双链核酸的电化学变性
US6207369B1 (en) * 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
DE69534386T2 (de) 1995-08-25 2006-04-20 Affymetrix, Inc. (a Delaware Corp.), Santa Clara Freigabe von intrazellulärem material
US6300063B1 (en) 1995-11-29 2001-10-09 Affymetrix, Inc. Polymorphism detection
US6953663B1 (en) 1995-11-29 2005-10-11 Affymetrix, Inc. Polymorphism detection
US6355436B1 (en) * 1996-05-17 2002-03-12 L'ecole Centrale De Lyon Method for analyzing biological substances in a conductive liquid medium
US6308847B1 (en) 1996-05-20 2001-10-30 Fresenius Kabi Aktiebolag Medical containers
GB9613980D0 (en) * 1996-07-02 1996-09-04 Scient Generics Ltd Denaturation of double-stranded nucleic acid
GB9614544D0 (en) * 1996-07-11 1996-09-04 Scient Generics Ltd Denaturation of double-stranded nucleic acid
US6635632B1 (en) 1996-12-23 2003-10-21 Athena Neurosciences, Inc. Cycloalkyl, lactam, lactone and related compounds, pharmaceutical compositions comprising same, and methods for inhibiting β-amyloid peptide release and/or its synthesis by use of such compounds
GB9706654D0 (en) * 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
AU735293B2 (en) * 1997-04-04 2001-07-05 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US5993611A (en) * 1997-09-24 1999-11-30 Sarnoff Corporation Capacitive denaturation of nucleic acid
US6884578B2 (en) 2000-03-31 2005-04-26 Affymetrix, Inc. Genes differentially expressed in secretory versus proliferative endometrium
US7108969B1 (en) 2000-09-08 2006-09-19 Affymetrix, Inc. Methods for detecting and diagnosing oral cancer
US6727060B2 (en) 2000-09-26 2004-04-27 Health Research, Inc. Analysis of HIV-1 coreceptor use in the clinical care of HIV-1-infected patients
US6670131B2 (en) * 2000-11-30 2003-12-30 Kabushiki Kaisha Toshiba Nucleic acid detection method and apparatus, and vessel for detecting nucleic acid
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
WO2003023360A2 (en) * 2001-09-10 2003-03-20 Meso Scale Technologies, Llc Methods and apparatus for conducting multiple measurements on a sample
US20050069926A1 (en) * 2003-08-01 2005-03-31 Affymetrix, Inc. Helicase-amplified reverse transcription
US7494791B2 (en) 2004-05-13 2009-02-24 Nanobiosym, Inc. Nano-PCR: methods and devices for nucleic acid amplification and detection
US20060264779A1 (en) 2005-05-09 2006-11-23 Kemp Timothy M Fluidic medical devices and uses thereof
US20070037169A1 (en) * 2005-08-09 2007-02-15 Combimatrix Corporation Selective Dehybridization using Electrochemically-Generated Reagent on an Electrode Microarray
EP1989326A4 (de) 2006-01-17 2009-09-30 Health Research Inc Heteroduplex-verfolgungstest
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
EP2016415B1 (de) 2006-04-21 2013-08-14 Nanobiosym, Inc. Einzelmolekülplattform für wirkstoffentdeckung: verfahren für wirkstoffentdeckung, einschliesslich der entdeckung von krebsmitteln und viruziden
AU2008276308A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
CA3170924A1 (en) 2007-10-02 2009-04-09 Labrador Diagnostics Llc Modular point-of-care devices and uses thereof
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
US9579402B2 (en) * 2009-12-04 2017-02-28 Biotium, Inc. Heterocycle-substituted xanthene dyes
BR112013018656B1 (pt) 2011-01-21 2021-03-02 Labrador Diagnostics Llc método para detectar a presença ou concentração de um analito numa amostra de fluido contido num recipiente, e, método de medição da concentração de analito numa amostra de fluido
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
AU2012318963B2 (en) 2011-09-25 2016-02-11 Labrador Diagnostics Llc Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
MX2015002919A (es) 2012-09-11 2015-08-14 Theranos Inc Sistemas y metodos de manejo de informacion que utilizan una firma biologica.
AU2014216060A1 (en) 2013-02-18 2015-08-20 Theranos Ip Company, Llc Systems and methods for collecting and transmitting assay results
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
US11545241B1 (en) 2013-09-07 2023-01-03 Labrador Diagnostics Llc Systems and methods for analyte testing and data management
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN109706222A (zh) 2013-12-11 2019-05-03 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2015175789A1 (en) 2014-05-14 2015-11-19 Mcruer Robert N Translocation control for sensing by a nanopore
US10125198B2 (en) 2015-04-17 2018-11-13 Distributed Bio, Inc. Method for mass humanization of non-human antibodies
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN108699505A (zh) 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
EP3497245A4 (de) 2016-08-15 2020-06-03 Accuragen Holdings Limited Zusammensetzungen und verfahren für den nachweis seltener sequenzvarianten
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US11179808B1 (en) * 2018-07-11 2021-11-23 Rosemount Aerospace Inc. System and method of additive manufacturing
CN113671167A (zh) * 2020-05-14 2021-11-19 北京元芯碳基集成电路研究院 生物电子器件及其制备方法和可控转化方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8417301D0 (en) * 1984-07-06 1984-08-08 Serono Diagnostics Ltd Assay
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5032570A (en) * 1987-08-04 1991-07-16 Hitachi Metals, Ltd. Method for producing ceramic superconducting material using intermediate products
US5527670A (en) * 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
AU8517391A (en) * 1990-09-12 1992-03-30 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5824477A (en) 1990-09-12 1998-10-20 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5565322A (en) 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
GB9201481D0 (en) * 1992-01-23 1992-03-11 Scient Generics Ltd Treatment of nucleic acid material
US6129828A (en) 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
CN1059238C (zh) * 1994-03-15 2000-12-06 科学通用有限公司 双链核酸的电化学变性
FR2722294B1 (fr) 1994-07-07 1996-10-04 Lyon Ecole Centrale Procede d'analyse qualitative et/ou quantitative de substances biologiques presentes dans un milieu liquide conducteur et capteurs biochimiques d'affinite utilises pour la mise en oeuvre de ce procede
US6013165A (en) 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method

Also Published As

Publication number Publication date
JPH09510107A (ja) 1997-10-14
WO1995025177A1 (en) 1995-09-21
CN1143979A (zh) 1997-02-26
EP0750680B1 (de) 1999-01-13
CN1059238C (zh) 2000-12-06
US6033850A (en) 2000-03-07
US6291185B1 (en) 2001-09-18
EP0750680A1 (de) 1997-01-02
DE69507289D1 (de) 1999-02-25
TW533236B (en) 2003-05-21
DE69507289T2 (de) 1999-07-08

Similar Documents

Publication Publication Date Title
ATE175723T1 (de) Elektrochemische denatusierung von doppelsträngiger nukleinsäure
ATE171221T1 (de) Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen
DE3688078T2 (de) Mittel zum elektrischen verbinden von elektroden an verschiedenen oberflaechen piezoelektrischer polymerfilme.
DE69637726D1 (de) Gruppen zum einfangen von nukleinsäuren
DE3582495D1 (de) Verfahren zur erhoehung der zellenspannung von elektrochemischen zellen.
DE3381783D1 (de) Menschliches antithrombin iii, dns sequenzen dafuer, expressions- und klonierungsvektoren die solche sequenzen enthalten und damit transformierte zellkulturen, verfahren zur expression von menschlichem antithrombin iii und diese enthaltende pharmazeutische zusammensetzungen.
DE69334211D1 (de) Multidrug resistenz gen
ES2147174T3 (es) Electroforesis que utiliza campos electricos de contorno fijado.
DE69530001D1 (de) Verbesserungen von oder in bezug auf endometrialfunktion
EP0150094A3 (de) Elektroden aus amorphem Kohlenstoff und ihre Verwendung in elektrochemischen Zellen
DK387186D0 (da) Fremgangsmaade til fremstilling af en elektrode og anvendelse deraf i elektrokemiske processer
FI915878A0 (fi) Menetelmä ja laite elektrolyyttiliuoksen käsittelemiseksi sähköisesti
ES2010390A6 (es) Procedimiento y aparato para llevar a cabo procesos electroquimicos.
ATE142020T1 (de) Verfahren zur haaranalyse
DE69733755D1 (de) Elektrode-einfang von nukleinsäuren
ATE227996T1 (de) Zusammensetzung und verfahren zur regulierung von zellproliferation und zelltod
DE3784983D1 (de) Elektrophorese, welche homogene oder inhomogene elektrische felder mit bestimmtem umkreis benutzt.
堀江登 et al. Studies on ATPase activity of actomyosin of squid mantle muscle.
BR8901157A (pt) Montagem de eletrodo para uma celula eletroquimica para reacao eletroquimica entre duas fases liquidas,cascata de celulas eletroquimicas e processo para realizar reacao eletroquimica entre duas fases liquidas
DE69612605T2 (de) Bipolartransistor für hohe Spannungen unter Benutzung von feldabgeschlossenen Anschlusselektroden
HUP0102413A1 (hu) Vektorok nukleinsavak bevitelére, ezeket tartalmazó készítmények és alkalmazásuk
ATE165631T1 (de) Electrolysezelle mit teilelektroden und zumindest einer gegenpoligen gegenelektrode
DE59002386D1 (de) Vorrichtung zur elektrokinetischen entsalzung von mauerwerken.
JPS6444993A (en) Aging of active matrix type liquid crystal display device
ITRM930015U1 (it) Apparato per elettroforesi in un gradiente di voltaggio.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties