ATE550441T1 - Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren - Google Patents

Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren

Info

Publication number
ATE550441T1
ATE550441T1 AT06803026T AT06803026T ATE550441T1 AT E550441 T1 ATE550441 T1 AT E550441T1 AT 06803026 T AT06803026 T AT 06803026T AT 06803026 T AT06803026 T AT 06803026T AT E550441 T1 ATE550441 T1 AT E550441T1
Authority
AT
Austria
Prior art keywords
compositions
methods
kits
nucleic acids
isothermal amplification
Prior art date
Application number
AT06803026T
Other languages
English (en)
Inventor
Michael Becker
Kristin Livezey
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE550441T1 publication Critical patent/ATE550441T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT06803026T 2005-09-06 2006-09-06 Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren ATE550441T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US71428105P 2005-09-06 2005-09-06
US72202805P 2005-09-29 2005-09-29
PCT/US2006/034663 WO2007030505A1 (en) 2005-09-06 2006-09-06 Methods, compositions and kits for isothermal amplification of nucleic acids

Publications (1)

Publication Number Publication Date
ATE550441T1 true ATE550441T1 (de) 2012-04-15

Family

ID=37607248

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06803026T ATE550441T1 (de) 2005-09-06 2006-09-06 Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren

Country Status (7)

Country Link
US (1) US20070054301A1 (de)
EP (1) EP1929045B9 (de)
JP (1) JP2009506788A (de)
AT (1) ATE550441T1 (de)
AU (1) AU2006287548A1 (de)
CA (1) CA2621146A1 (de)
WO (1) WO2007030505A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1356094B1 (de) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
EP1366197B1 (de) * 2001-03-09 2007-05-09 Nugen Technologies, Inc. Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
EP1929046B1 (de) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
EP2250283A2 (de) * 2008-02-12 2010-11-17 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur isothermen amplifikation von nukleinsäuren
US7846666B2 (en) * 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
EP2279403B1 (de) 2008-05-05 2016-03-16 Los Alamos National Security, LLC Höchst vereinfachte vorbereitung von nukleinsäureproben auf lateralflussbasis und passive strömungssteuerung
KR20100019220A (ko) * 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
JP2013515458A (ja) * 2009-10-29 2013-05-09 バイオ−ラッド ラボラトリーズ インコーポレイテッド 細胞培養試料中のマイコプラズマ汚染の迅速な検出
EP2369325A1 (de) 2010-03-12 2011-09-28 Eppendorf Ag Arrayanalyse für Online-Detektion
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
EP3564392B1 (de) * 2010-12-17 2021-11-24 Life Technologies Corporation Verfahren für nukleinsäureamplifikation
GB201101488D0 (en) * 2011-01-28 2011-03-16 Ge Healthcare Ltd Container storing freeze dried biological sample
DK2699700T3 (en) 2011-04-20 2016-08-01 Mesa Biotech Inc Integrated device for nukleinsyreregistrering and identification
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
SG11201402760VA (en) 2011-12-01 2014-06-27 Genapsys Inc Systems and methods for high efficiency electronic sequencing and detection
US10975423B2 (en) * 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US10590474B2 (en) * 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
JP2016510991A (ja) * 2013-03-11 2016-04-14 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 正確な鎖置換型等温増幅のための方法
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
ES2908751T3 (es) * 2013-03-15 2022-05-03 Labrador Diagnostics Llc Amplificación de ácidos nucleicos
WO2014152625A1 (en) 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
CN116334193A (zh) 2013-03-15 2023-06-27 赛拉诺斯知识产权有限责任公司 核酸扩增
CA2906824C (en) 2013-03-15 2023-10-03 Theranos, Inc. Nucleic acid amplification
AU2014317990A1 (en) 2013-09-06 2016-02-25 Theranos Ip Company, Llc Systems and methods for detecting infectious diseases
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015116686A1 (en) 2014-01-29 2015-08-06 Agilent Technologies, Inc. Cas9-based isothermal method of detection of specific dna sequence
EP3556864B1 (de) 2014-04-18 2020-12-09 Genapsys, Inc. Verfahren und systeme zur nukleinsäureamplifikation
CN107810060A (zh) 2015-04-24 2018-03-16 美飒生物技术公司 流体检测盒
CN116397014A (zh) 2016-07-20 2023-07-07 测序健康公司 用于核酸测序的系统和方法
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP3530755B1 (de) * 2018-02-26 2020-08-26 AGCT GmbH Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung
GB201804585D0 (en) * 2018-03-22 2018-05-09 Dnae Diagnostics Ltd Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq)
WO2020092255A1 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Accelerated polymerase chain reaction
CN112029905B (zh) * 2020-08-18 2023-08-11 珠海赛乐奇生物技术股份有限公司 一种检测torch病原体的基因芯片和试剂盒
IL307172A (en) 2021-03-30 2023-11-01 Illumina Inc Improved methods of isothermal complementary DNA and library preparation

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683196A (en) * 1983-12-12 1987-07-28 Meru, Inc. Method and materials for the identification of lipopolysaccharide producing microorganisms
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP2774121B2 (ja) * 1987-07-31 1998-07-09 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 標的ポリヌクレオチド配列の選択的増幅
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5516663A (en) * 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DK0549107T3 (da) * 1991-10-11 1998-08-31 Dade Behring Marburg Gmbh Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
EP0702728B1 (de) * 1993-06-09 1998-04-15 Gamera Bioscience Corporation Magnetische zyklusreaktionen
WO1995005480A2 (en) * 1993-08-18 1995-02-23 Id Biomedical Corporation Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
CA2185239C (en) * 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
FR2721945B1 (fr) 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
US5545539A (en) * 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
US6270962B1 (en) * 1995-01-30 2001-08-07 The Regents Of The University Of California Methods for the elimination of DNA sequencing artifacts
AT402203B (de) * 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
US6114150A (en) * 1995-11-29 2000-09-05 Yale University Amplification of nucleic acids
CA2249717A1 (en) * 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US6150094A (en) * 1996-05-23 2000-11-21 Qiagen Gmbh Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6242235B1 (en) * 1998-06-24 2001-06-05 Promega Corp. Polymerase stabilization by polyethoxylated amine surfactants
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
ES2369818T3 (es) 1998-11-09 2011-12-07 Eiken Kagaku Kabushiki Kaisha Procedimiento de síntesis de ácido nucleico.
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
CN102586228A (zh) * 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001055454A1 (en) * 2000-01-28 2001-08-02 Althea Technologies, Inc. Methods for analysis of gene expression
DE60136166D1 (de) * 2000-02-07 2008-11-27 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
AU3806701A (en) * 2000-02-07 2001-08-14 Illumina Inc Nucleic acid detection methods using universal priming
ES2464132T3 (es) * 2000-04-07 2014-05-30 Eiken Kagaku Kabushiki Kaisha Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario
CA2460639C (en) * 2001-09-19 2013-07-16 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
CA2491023A1 (en) * 2002-07-19 2004-01-29 Althea Technologies, Inc. Strategies for gene expression analysis
US7282328B2 (en) * 2002-09-20 2007-10-16 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2004042034A2 (en) * 2002-11-04 2004-05-21 Fred Hutchinson Cancer Research Center Method for the linear amplification of complementary dna
CA2898814C (en) * 2004-08-27 2015-12-29 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature

Also Published As

Publication number Publication date
EP1929045B9 (de) 2012-06-20
AU2006287548A1 (en) 2007-03-15
CA2621146A1 (en) 2007-03-15
WO2007030505A1 (en) 2007-03-15
EP1929045B1 (de) 2012-03-21
US20070054301A1 (en) 2007-03-08
EP1929045A1 (de) 2008-06-11
JP2009506788A (ja) 2009-02-19

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