ATE550441T1 - Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren - Google Patents
Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäurenInfo
- Publication number
- ATE550441T1 ATE550441T1 AT06803026T AT06803026T ATE550441T1 AT E550441 T1 ATE550441 T1 AT E550441T1 AT 06803026 T AT06803026 T AT 06803026T AT 06803026 T AT06803026 T AT 06803026T AT E550441 T1 ATE550441 T1 AT E550441T1
- Authority
- AT
- Austria
- Prior art keywords
- compositions
- methods
- kits
- nucleic acids
- isothermal amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US71428105P | 2005-09-06 | 2005-09-06 | |
US72202805P | 2005-09-29 | 2005-09-29 | |
PCT/US2006/034663 WO2007030505A1 (en) | 2005-09-06 | 2006-09-06 | Methods, compositions and kits for isothermal amplification of nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE550441T1 true ATE550441T1 (de) | 2012-04-15 |
Family
ID=37607248
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06803026T ATE550441T1 (de) | 2005-09-06 | 2006-09-06 | Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren |
Country Status (7)
Country | Link |
---|---|
US (1) | US20070054301A1 (de) |
EP (1) | EP1929045B9 (de) |
JP (1) | JP2009506788A (de) |
AT (1) | ATE550441T1 (de) |
AU (1) | AU2006287548A1 (de) |
CA (1) | CA2621146A1 (de) |
WO (1) | WO2007030505A1 (de) |
Families Citing this family (44)
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US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
EP1356094B1 (de) * | 2000-06-26 | 2010-01-13 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
CA2430329A1 (en) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US8507662B2 (en) | 2001-01-19 | 2013-08-13 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
ATE361996T1 (de) | 2001-03-09 | 2007-06-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen |
CA2521084A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
WO2007030759A2 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
WO2008105814A2 (en) | 2006-08-22 | 2008-09-04 | Los Alamos National Security, Llc | Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
WO2009102896A2 (en) * | 2008-02-12 | 2009-08-20 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009117698A2 (en) * | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
CN102084238B (zh) | 2008-05-05 | 2016-06-01 | 洛斯阿拉莫斯国家安全有限责任公司 | 基于高度简化的侧向流动的核酸样品制备和被动流体流动控制 |
KR20100019220A (ko) * | 2008-08-08 | 2010-02-18 | 삼성전자주식회사 | 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법 |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
EP2494079A4 (de) * | 2009-10-29 | 2013-06-26 | Bio Rad Laboratories | Schnelle erkennung von mycoplasma-kontamination in zellkulturproben |
EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
SG189839A1 (en) | 2010-10-04 | 2013-06-28 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
CN110079588B (zh) * | 2010-12-17 | 2024-03-15 | 生命技术公司 | 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 |
GB201101488D0 (en) * | 2011-01-28 | 2011-03-16 | Ge Healthcare Ltd | Container storing freeze dried biological sample |
KR20140044323A (ko) | 2011-04-20 | 2014-04-14 | 메사 테크 인터내셔널, 인코포레이티드 | 핵산의 왕복 증폭 반응 |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
EP2785868B1 (de) | 2011-12-01 | 2017-04-12 | Genapsys Inc. | Systeme und verfahren für hocheffiziente elektronische sequenzierung und nachweis |
US10590474B2 (en) * | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
WO2014164479A1 (en) * | 2013-03-11 | 2014-10-09 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
US10975423B2 (en) * | 2013-03-11 | 2021-04-13 | Elitechgroup, Inc. | Methods for true isothermal strand displacement amplification |
WO2014158628A1 (en) | 2013-03-14 | 2014-10-02 | Hologic, Inc. | Compositions and methods for analysis of nucleic acid molecules |
ES2881080T3 (es) | 2013-03-15 | 2021-11-26 | Labrador Diagnostics Llc | Amplificación de ácidos nucleicos |
WO2014152625A1 (en) | 2013-03-15 | 2014-09-25 | Genapsys, Inc. | Systems and methods for biological analysis |
AU2014233152A1 (en) * | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
ES2738602T3 (es) | 2013-03-15 | 2020-01-24 | Theranos Ip Co Llc | Amplificación de ácidos nucleicos |
KR20160053979A (ko) | 2013-09-06 | 2016-05-13 | 테라노스, 인코포레이티드 | 전염성 질환의 검출을 위한 시스템 및 방법 |
WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
US9850525B2 (en) | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
WO2015161054A2 (en) | 2014-04-18 | 2015-10-22 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
CN118788410A (zh) | 2015-04-24 | 2024-10-18 | 美飒生物技术公司 | 流体检测盒 |
CN116397014A (zh) | 2016-07-20 | 2023-07-07 | 测序健康公司 | 用于核酸测序的系统和方法 |
CN111566224A (zh) | 2017-09-21 | 2020-08-21 | 吉纳普赛斯股份有限公司 | 用于核酸测序的系统和方法 |
EP3530755B1 (de) | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
GB201804585D0 (en) * | 2018-03-22 | 2018-05-09 | Dnae Diagnostics Ltd | Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq) |
US20210388431A1 (en) * | 2018-10-29 | 2021-12-16 | Igor V. Kutyavin | Thermokinetically balanced isothermal amplification of nucleic acid sequences |
CN112029905B (zh) * | 2020-08-18 | 2023-08-11 | 珠海赛乐奇生物技术股份有限公司 | 一种检测torch病原体的基因芯片和试剂盒 |
IL307172A (en) | 2021-03-30 | 2023-11-01 | Illumina Inc | Improved methods of isothermal complementary DNA and library preparation |
Family Cites Families (44)
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US4683196A (en) * | 1983-12-12 | 1987-07-28 | Meru, Inc. | Method and materials for the identification of lipopolysaccharide producing microorganisms |
US4786600A (en) * | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
CA1340843C (en) * | 1987-07-31 | 1999-12-07 | J. Lawrence Burg | Selective amplification of target polynucleotide sequences |
US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
CA1339731C (en) * | 1988-10-12 | 1998-03-17 | Charles T. Caskey | Multiplex genomic dna amplification for deletion detection |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
CA2080305A1 (en) * | 1991-10-11 | 1993-04-12 | Linda M. Western | Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification |
US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
EP0702728B1 (de) * | 1993-06-09 | 1998-04-15 | Gamera Bioscience Corporation | Magnetische zyklusreaktionen |
EP0716713A1 (de) * | 1993-08-18 | 1996-06-19 | Id Biomedical Corporation | Zusammensetzungen und Verfahren zur Detektion von gezielten DNS Sequenzen durch Nützung von Molekülen mit Enzymen und damit angrenzenden Sequenzen |
US5834252A (en) * | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
CA2185239C (en) * | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
US6100078A (en) * | 1994-04-01 | 2000-08-08 | Gen-Probe Incorporated | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
FR2721945B1 (fr) | 1994-07-04 | 1996-10-18 | David Fabrice | Accroissement genique, un procede d'amplicication genique isotherme et ses applications |
US5545539A (en) * | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
US6270962B1 (en) * | 1995-01-30 | 2001-08-07 | The Regents Of The University Of California | Methods for the elimination of DNA sequencing artifacts |
AT402203B (de) * | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
US6114150A (en) * | 1995-11-29 | 2000-09-05 | Yale University | Amplification of nucleic acids |
AU723678B2 (en) * | 1996-03-18 | 2000-08-31 | Molecular Biology Resources, Inc. | Target nucleic acid sequence amplification |
US6150094A (en) * | 1996-05-23 | 2000-11-21 | Qiagen Gmbh | Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces |
US6030814A (en) * | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
US6743605B1 (en) * | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
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EP1873260B1 (de) | 1998-11-09 | 2009-03-18 | Eiken Kagaku Kabushiki Kaisha | Prozess zur Synthetisierung von Nukleinsäure |
US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
BR0014182A (pt) * | 1999-09-13 | 2002-05-21 | Nugen Technologies Inc | Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6618679B2 (en) * | 2000-01-28 | 2003-09-09 | Althea Technologies, Inc. | Methods for analysis of gene expression |
DE60127939T2 (de) * | 2000-02-07 | 2008-01-24 | Illumina, Inc., San Diego | Nukleinsäure-Nachweisverfahren mit universellem Priming |
US20020006617A1 (en) * | 2000-02-07 | 2002-01-17 | Jian-Bing Fan | Nucleic acid detection methods using universal priming |
WO2001077317A1 (en) * | 2000-04-07 | 2001-10-18 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
DK1436404T3 (da) * | 2001-09-19 | 2010-03-08 | Alexion Pharma Inc | Manipulerede templates og deres anvendelse i single-primer amplifikation |
AU2003261168A1 (en) * | 2002-07-19 | 2004-02-09 | Althea Technologies, Inc. | Strategies for gene expression analysis |
WO2004027025A2 (en) * | 2002-09-20 | 2004-04-01 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
WO2004042034A2 (en) * | 2002-11-04 | 2004-05-21 | Fred Hutchinson Cancer Research Center | Method for the linear amplification of complementary dna |
DE602005026730D1 (de) * | 2004-08-27 | 2011-04-14 | Gen Probe Inc | Einfach-Primernukleinsäuren-Erweiterungsverfahren |
US20060172324A1 (en) * | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
-
2006
- 2006-09-06 AT AT06803026T patent/ATE550441T1/de active
- 2006-09-06 CA CA002621146A patent/CA2621146A1/en not_active Abandoned
- 2006-09-06 JP JP2008530153A patent/JP2009506788A/ja not_active Withdrawn
- 2006-09-06 US US11/517,117 patent/US20070054301A1/en not_active Abandoned
- 2006-09-06 AU AU2006287548A patent/AU2006287548A1/en not_active Abandoned
- 2006-09-06 WO PCT/US2006/034663 patent/WO2007030505A1/en active Application Filing
- 2006-09-06 EP EP06803026A patent/EP1929045B9/de not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
US20070054301A1 (en) | 2007-03-08 |
EP1929045B9 (de) | 2012-06-20 |
AU2006287548A1 (en) | 2007-03-15 |
CA2621146A1 (en) | 2007-03-15 |
EP1929045B1 (de) | 2012-03-21 |
JP2009506788A (ja) | 2009-02-19 |
WO2007030505A1 (en) | 2007-03-15 |
EP1929045A1 (de) | 2008-06-11 |
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