ATE554187T1 - Verfahren für die schmelzanalyse der nukleinsäuren - Google Patents
Verfahren für die schmelzanalyse der nukleinsäurenInfo
- Publication number
- ATE554187T1 ATE554187T1 AT08731668T AT08731668T ATE554187T1 AT E554187 T1 ATE554187 T1 AT E554187T1 AT 08731668 T AT08731668 T AT 08731668T AT 08731668 T AT08731668 T AT 08731668T AT E554187 T1 ATE554187 T1 AT E554187T1
- Authority
- AT
- Austria
- Prior art keywords
- primer
- probe element
- nucleic acid
- nucleic acids
- specific
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 238000002844 melting Methods 0.000 title abstract 3
- 230000008018 melting Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000000523 sample Substances 0.000 abstract 4
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US90572107P | 2007-03-08 | 2007-03-08 | |
| PCT/US2008/056217 WO2008109823A2 (en) | 2007-03-08 | 2008-03-07 | Primers for melting analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE554187T1 true ATE554187T1 (de) | 2012-05-15 |
Family
ID=39739135
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08731668T ATE554187T1 (de) | 2007-03-08 | 2008-03-07 | Verfahren für die schmelzanalyse der nukleinsäuren |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8399189B2 (de) |
| EP (1) | EP2134861B1 (de) |
| JP (1) | JP5424903B2 (de) |
| CN (1) | CN101918587B (de) |
| AT (1) | ATE554187T1 (de) |
| AU (1) | AU2008222649B2 (de) |
| CA (1) | CA2680121C (de) |
| DK (1) | DK2134861T3 (de) |
| ES (1) | ES2386487T3 (de) |
| WO (1) | WO2008109823A2 (de) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE554187T1 (de) | 2007-03-08 | 2012-05-15 | Idaho Technology | Verfahren für die schmelzanalyse der nukleinsäuren |
| US9422597B2 (en) | 2008-11-07 | 2016-08-23 | Biofire Diagnostics, Inc. | Allele amplification bias |
| WO2010135917A1 (zh) | 2009-05-26 | 2010-12-02 | 厦门大学 | 一种检测核酸序列变异的方法 |
| US8815515B1 (en) | 2009-09-24 | 2014-08-26 | University Of Utah Research Foundation | Methods, compositions, and kits for rare allele detection |
| GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| CN103635593B (zh) * | 2011-05-04 | 2018-03-20 | 生物概念股份有限公司 | 用于检测核酸序列变体的方法 |
| EP2740804A4 (de) * | 2011-08-05 | 2015-06-24 | Toshiba Kk | Verfahren für nukleinsäureanalyse |
| KR101357237B1 (ko) | 2011-12-09 | 2014-02-06 | 한국 한의학 연구원 | Ｐｃｒ 앰플리콘의 희석 단계를 포함하는 ｄｎａ 용융 분석을 이용한 ｓｎｐ 지노타이핑 방법 |
| CN102643910B (zh) * | 2012-04-10 | 2014-10-15 | 中国人民解放军第三军医大学第一附属医院 | 非对称多色荧光发夹探针链反应在病原菌检测中的应用 |
| WO2014043660A2 (en) * | 2012-09-17 | 2014-03-20 | Brandeis University | Combination of dsdna binding dye and probes for characterization of ssdna sequences |
| EP3039594B1 (de) | 2013-08-30 | 2019-10-09 | University Of Utah Research Foundation | Quantenverfahren für fluoreszenzhintergrundbeseitigung in der dna-schmelzanalyse |
| GB201411567D0 (en) * | 2014-06-30 | 2014-08-13 | Epistem Ltd | Quantification methods |
| EP4613872A3 (de) * | 2014-08-11 | 2025-11-12 | Luminex Corporation | Sonden zur verbesserten schmelzunterscheidung und multiplexierung in nukleinsäuretests |
| US20170335382A1 (en) * | 2014-11-10 | 2017-11-23 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isothermal Amplification Assay for the Detection of Short Nucleic Acid Sequences |
| KR102266987B1 (ko) * | 2014-12-24 | 2021-06-21 | 주식회사 시선바이오머티리얼스 | Pna 프로브를 이용한 인공합성 올리고의 품질보증 방법 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| US10961573B2 (en) | 2016-03-28 | 2021-03-30 | Boreal Genomics, Inc. | Linked duplex target capture |
| US11268137B2 (en) | 2016-12-09 | 2022-03-08 | Boreal Genomics, Inc. | Linked ligation |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN109055526A (zh) * | 2018-08-17 | 2018-12-21 | 潍坊德诺泰克生物科技有限公司 | 用于检测rs662的引物探针组及其应用 |
| US12098419B2 (en) | 2018-08-23 | 2024-09-24 | Ncan Genomics, Inc. | Linked target capture and ligation |
| WO2020141464A1 (en) | 2019-01-03 | 2020-07-09 | Boreal Genomics, Inc. | Linked target capture |
| CN110257492A (zh) * | 2019-07-04 | 2019-09-20 | 黄劭 | 一组fret杂交扩增引物和检测探针组 |
| AU2022426779A1 (en) * | 2021-12-29 | 2024-07-18 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
| CN115728276B (zh) * | 2022-11-14 | 2024-01-23 | 中船重工安谱（湖北）仪器有限公司 | 爆炸物检测方法和检测系统 |
| CN118866099B (zh) * | 2024-09-03 | 2025-07-29 | 国际遗传工程和生物技术中心泰州区域研究中心 | 设计Taqman SNP基因分型探针及引物组的方法、系统和存储介质 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPS076802A0 (en) * | 2002-02-26 | 2002-03-21 | Commonwealth Scientific And Industrial Research Organisation | Selective nucleic acid amplification |
| EP1499745B1 (de) | 2002-04-26 | 2016-11-23 | University of Utah Research Foundation | Charakterisierung einzelsträngiger nukleinsäuren durch schmelzanalyse von sekundärstrukturen unter verwendung eines strangspezifischen nukleinsäurefarbstoffs |
| WO2004038038A2 (en) | 2002-10-23 | 2004-05-06 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
| US7456281B2 (en) | 2005-04-20 | 2008-11-25 | Idaho Technology, Inc. | Nucleic acid melting analysis with saturation dyes |
| EP1885881A1 (de) | 2005-05-11 | 2008-02-13 | Stratagene California | Snapback-oligonukleotidsonde |
| EP1927064B1 (de) | 2005-09-20 | 2016-12-14 | University of Utah Research Foundation | Schmelzkurvenanalyse mit exponentieller hintergrundsubtraktion |
| ATE554187T1 (de) | 2007-03-08 | 2012-05-15 | Idaho Technology | Verfahren für die schmelzanalyse der nukleinsäuren |
| EP2336358A1 (de) * | 2008-05-06 | 2011-06-22 | QIAGEN GmbH | Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion |
-
2008
- 2008-03-07 AT AT08731668T patent/ATE554187T1/de active
- 2008-03-07 EP EP08731668A patent/EP2134861B1/de active Active
- 2008-03-07 ES ES08731668T patent/ES2386487T3/es active Active
- 2008-03-07 US US12/530,052 patent/US8399189B2/en active Active
- 2008-03-07 WO PCT/US2008/056217 patent/WO2008109823A2/en not_active Ceased
- 2008-03-07 CN CN2008800148410A patent/CN101918587B/zh active Active
- 2008-03-07 AU AU2008222649A patent/AU2008222649B2/en active Active
- 2008-03-07 DK DK08731668.3T patent/DK2134861T3/da active
- 2008-03-07 JP JP2009552907A patent/JP5424903B2/ja active Active
- 2008-03-07 CA CA2680121A patent/CA2680121C/en active Active
-
2013
- 2013-02-19 US US13/770,539 patent/US9771610B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2134861A4 (de) | 2010-03-03 |
| WO2008109823A2 (en) | 2008-09-12 |
| US8399189B2 (en) | 2013-03-19 |
| US20160348157A1 (en) | 2016-12-01 |
| AU2008222649A1 (en) | 2008-09-12 |
| AU2008222649B2 (en) | 2014-06-12 |
| CA2680121C (en) | 2016-05-10 |
| CN101918587A (zh) | 2010-12-15 |
| ES2386487T3 (es) | 2012-08-21 |
| US20100196890A1 (en) | 2010-08-05 |
| EP2134861A2 (de) | 2009-12-23 |
| CN101918587B (zh) | 2013-03-27 |
| JP2010520756A (ja) | 2010-06-17 |
| JP5424903B2 (ja) | 2014-02-26 |
| CA2680121A1 (en) | 2008-09-12 |
| EP2134861B1 (de) | 2012-04-18 |
| US9771610B2 (en) | 2017-09-26 |
| AU2008222649A8 (en) | 2011-01-27 |
| DK2134861T3 (da) | 2012-07-30 |
| WO2008109823A3 (en) | 2008-11-13 |
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