CN101918587A - 用于熔解分析的引物 - Google Patents
用于熔解分析的引物 Download PDFInfo
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- CN101918587A CN101918587A CN2008800148410A CN200880014841A CN101918587A CN 101918587 A CN101918587 A CN 101918587A CN 2008800148410 A CN2008800148410 A CN 2008800148410A CN 200880014841 A CN200880014841 A CN 200880014841A CN 101918587 A CN101918587 A CN 101918587A
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- primer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
Description
Claims (41)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US90572107P | 2007-03-08 | 2007-03-08 | |
US60/905,721 | 2007-03-08 | ||
PCT/US2008/056217 WO2008109823A2 (en) | 2007-03-08 | 2008-03-07 | Primers for melting analysis |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101918587A true CN101918587A (zh) | 2010-12-15 |
CN101918587B CN101918587B (zh) | 2013-03-27 |
Family
ID=39739135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2008800148410A Active CN101918587B (zh) | 2007-03-08 | 2008-03-07 | 用于熔解分析的引物 |
Country Status (10)
Country | Link |
---|---|
US (2) | US8399189B2 (zh) |
EP (1) | EP2134861B1 (zh) |
JP (1) | JP5424903B2 (zh) |
CN (1) | CN101918587B (zh) |
AT (1) | ATE554187T1 (zh) |
AU (1) | AU2008222649B2 (zh) |
CA (1) | CA2680121C (zh) |
DK (1) | DK2134861T3 (zh) |
ES (1) | ES2386487T3 (zh) |
WO (1) | WO2008109823A2 (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102643910A (zh) * | 2012-04-10 | 2012-08-22 | 中国人民解放军第三军医大学第一附属医院 | 非对称多色荧光发夹探针链反应在病原菌检测中的应用 |
CN103635593A (zh) * | 2011-05-04 | 2014-03-12 | 生物概念股份有限公司 | 用于检测核酸序列变体的方法 |
CN109055526A (zh) * | 2018-08-17 | 2018-12-21 | 潍坊德诺泰克生物科技有限公司 | 用于检测rs662的引物探针组及其应用 |
CN110257492A (zh) * | 2019-07-04 | 2019-09-20 | 黄劭 | 一组fret杂交扩增引物和检测探针组 |
CN110621783A (zh) * | 2017-05-25 | 2019-12-27 | 豪夫迈·罗氏有限公司 | 多重核酸扩增测定 |
CN115728276A (zh) * | 2022-11-14 | 2023-03-03 | 中船重工安谱(湖北)仪器有限公司 | 爆炸物检测方法和检测系统 |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5424903B2 (ja) | 2007-03-08 | 2014-02-26 | バイオファイアー ダイアグノスティックス,インコーポレイテッド | 融解解析のためのプライマー |
US9422597B2 (en) | 2008-11-07 | 2016-08-23 | Biofire Diagnostics, Inc. | Allele amplification bias |
US8691504B2 (en) | 2009-05-26 | 2014-04-08 | Xiamen University | Method for detecting variations in nucleic acid sequences |
US8815515B1 (en) | 2009-09-24 | 2014-08-26 | University Of Utah Research Foundation | Methods, compositions, and kits for rare allele detection |
GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
WO2013021841A1 (ja) * | 2011-08-05 | 2013-02-14 | 株式会社 東芝 | 核酸解析法 |
KR101357237B1 (ko) | 2011-12-09 | 2014-02-06 | 한국 한의학 연구원 | Pcr 앰플리콘의 희석 단계를 포함하는 dna 용융 분석을 이용한 snp 지노타이핑 방법 |
CN104995312A (zh) * | 2012-09-17 | 2015-10-21 | 布兰代斯大学 | 用于表征ssdna序列的dsdna结合染料和探针的组合 |
US11066696B2 (en) | 2013-08-30 | 2021-07-20 | University Of Utah Research Foundation | Quantum method for fluorescence background removal in DNA melting analysis |
GB201411567D0 (en) * | 2014-06-30 | 2014-08-13 | Epistem Ltd | Quantification methods |
EP4299760A3 (en) | 2014-08-11 | 2024-02-21 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
US20170335382A1 (en) * | 2014-11-10 | 2017-11-23 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isothermal Amplification Assay for the Detection of Short Nucleic Acid Sequences |
KR102266987B1 (ko) * | 2014-12-24 | 2021-06-21 | 주식회사 시선바이오머티리얼스 | Pna 프로브를 이용한 인공합성 올리고의 품질보증 방법 |
CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
US10961573B2 (en) | 2016-03-28 | 2021-03-30 | Boreal Genomics, Inc. | Linked duplex target capture |
CA3048420A1 (en) | 2016-12-09 | 2018-06-14 | Boreal Genomics, Inc. | Linked ligation |
US11473136B2 (en) | 2019-01-03 | 2022-10-18 | Ncan Genomics, Inc. | Linked target capture |
US20230279479A1 (en) * | 2021-12-29 | 2023-09-07 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPS076802A0 (en) * | 2002-02-26 | 2002-03-21 | Commonwealth Scientific And Industrial Research Organisation | Selective nucleic acid amplification |
US7297484B2 (en) * | 2002-04-26 | 2007-11-20 | Idaho Technology | Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye |
CA2501144C (en) | 2002-10-23 | 2015-10-06 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
US7456281B2 (en) | 2005-04-20 | 2008-11-25 | Idaho Technology, Inc. | Nucleic acid melting analysis with saturation dyes |
AU2006244035A1 (en) * | 2005-05-11 | 2006-11-16 | Stratagene California | Snapback oligonucleotide probe |
CA2623268C (en) | 2005-09-20 | 2021-12-14 | University Of Utah Research Foundation | Melting curve analysis with exponential background subtraction |
JP5424903B2 (ja) | 2007-03-08 | 2014-02-26 | バイオファイアー ダイアグノスティックス,インコーポレイテッド | 融解解析のためのプライマー |
EP2116614A1 (en) * | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
-
2008
- 2008-03-07 JP JP2009552907A patent/JP5424903B2/ja active Active
- 2008-03-07 US US12/530,052 patent/US8399189B2/en active Active
- 2008-03-07 CA CA2680121A patent/CA2680121C/en active Active
- 2008-03-07 DK DK08731668.3T patent/DK2134861T3/da active
- 2008-03-07 AT AT08731668T patent/ATE554187T1/de active
- 2008-03-07 CN CN2008800148410A patent/CN101918587B/zh active Active
- 2008-03-07 EP EP08731668A patent/EP2134861B1/en active Active
- 2008-03-07 WO PCT/US2008/056217 patent/WO2008109823A2/en active Application Filing
- 2008-03-07 ES ES08731668T patent/ES2386487T3/es active Active
- 2008-03-07 AU AU2008222649A patent/AU2008222649B2/en active Active
-
2013
- 2013-02-19 US US13/770,539 patent/US9771610B2/en active Active
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103635593A (zh) * | 2011-05-04 | 2014-03-12 | 生物概念股份有限公司 | 用于检测核酸序列变体的方法 |
CN103635593B (zh) * | 2011-05-04 | 2018-03-20 | 生物概念股份有限公司 | 用于检测核酸序列变体的方法 |
CN102643910A (zh) * | 2012-04-10 | 2012-08-22 | 中国人民解放军第三军医大学第一附属医院 | 非对称多色荧光发夹探针链反应在病原菌检测中的应用 |
CN102643910B (zh) * | 2012-04-10 | 2014-10-15 | 中国人民解放军第三军医大学第一附属医院 | 非对称多色荧光发夹探针链反应在病原菌检测中的应用 |
CN110621783A (zh) * | 2017-05-25 | 2019-12-27 | 豪夫迈·罗氏有限公司 | 多重核酸扩增测定 |
CN110621783B (zh) * | 2017-05-25 | 2024-05-10 | 豪夫迈·罗氏有限公司 | 多重核酸扩增测定 |
CN109055526A (zh) * | 2018-08-17 | 2018-12-21 | 潍坊德诺泰克生物科技有限公司 | 用于检测rs662的引物探针组及其应用 |
CN110257492A (zh) * | 2019-07-04 | 2019-09-20 | 黄劭 | 一组fret杂交扩增引物和检测探针组 |
CN115728276A (zh) * | 2022-11-14 | 2023-03-03 | 中船重工安谱(湖北)仪器有限公司 | 爆炸物检测方法和检测系统 |
CN115728276B (zh) * | 2022-11-14 | 2024-01-23 | 中船重工安谱(湖北)仪器有限公司 | 爆炸物检测方法和检测系统 |
Also Published As
Publication number | Publication date |
---|---|
AU2008222649A1 (en) | 2008-09-12 |
US20160348157A1 (en) | 2016-12-01 |
EP2134861B1 (en) | 2012-04-18 |
CN101918587B (zh) | 2013-03-27 |
AU2008222649A8 (en) | 2011-01-27 |
EP2134861A4 (en) | 2010-03-03 |
ATE554187T1 (de) | 2012-05-15 |
AU2008222649B2 (en) | 2014-06-12 |
EP2134861A2 (en) | 2009-12-23 |
JP5424903B2 (ja) | 2014-02-26 |
CA2680121C (en) | 2016-05-10 |
US8399189B2 (en) | 2013-03-19 |
ES2386487T3 (es) | 2012-08-21 |
DK2134861T3 (da) | 2012-07-30 |
WO2008109823A3 (en) | 2008-11-13 |
US9771610B2 (en) | 2017-09-26 |
WO2008109823A2 (en) | 2008-09-12 |
US20100196890A1 (en) | 2010-08-05 |
CA2680121A1 (en) | 2008-09-12 |
JP2010520756A (ja) | 2010-06-17 |
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Address after: American Utah Applicant after: Idaho Technology Inc Applicant after: Univ Utah Res Found Address before: American Utah Applicant before: Idaho Technology Applicant before: Univ Utah Res Found |
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Address after: Delaware Co-patentee after: Univ Utah Res Found Patentee after: Baiou law protection limited liability company Address before: American Utah Co-patentee before: Univ Utah Res Found Patentee before: Idaho Technology Inc |