ATE393244T1 - Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit - Google Patents

Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit

Info

Publication number
ATE393244T1
ATE393244T1 AT06001513T AT06001513T ATE393244T1 AT E393244 T1 ATE393244 T1 AT E393244T1 AT 06001513 T AT06001513 T AT 06001513T AT 06001513 T AT06001513 T AT 06001513T AT E393244 T1 ATE393244 T1 AT E393244T1
Authority
AT
Austria
Prior art keywords
melting temperature
genotyping
temperature dependence
identification
nucleic acid
Prior art date
Application number
AT06001513T
Other languages
English (en)
Inventor
Soren Germer
Russell Gene Higuchi
Daniel B Mirel
Jun Wang
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE393244T1 publication Critical patent/ATE393244T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT06001513T 2005-01-28 2006-01-25 Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit ATE393244T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64794805P 2005-01-28 2005-01-28
US66018705P 2005-03-09 2005-03-09

Publications (1)

Publication Number Publication Date
ATE393244T1 true ATE393244T1 (de) 2008-05-15

Family

ID=36250864

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06001513T ATE393244T1 (de) 2005-01-28 2006-01-25 Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit

Country Status (7)

Country Link
US (1) US20060172324A1 (de)
EP (1) EP1686190B1 (de)
JP (1) JP4351217B2 (de)
AT (1) ATE393244T1 (de)
CA (1) CA2532160C (de)
DE (1) DE602006000944T2 (de)
ES (1) ES2304739T3 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE550441T1 (de) * 2005-09-06 2012-04-15 Gen Probe Inc Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
EP2226390B1 (de) * 2007-12-26 2018-08-29 ARKRAY, Inc. Verfahren und vorrichtung zur schmelzkurvenanalyse
KR20100080621A (ko) 2008-07-02 2010-07-09 아크레이 가부시키가이샤 표적 핵산 서열의 증폭 방법, 그것을 사용한 변이의 검출 방법, 및, 그것에 사용하는 시약
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
WO2010070366A1 (en) * 2008-12-15 2010-06-24 Debreceni Egyetem Cytometric method for the comparative analysis of the length of pcr products and uses of this method
EP2894226B1 (de) 2009-01-08 2021-04-14 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzung zur verbesserung der wirksamkeit bei einer nukleinsäureverstärkungsreaktion
EP2450443B1 (de) * 2010-01-21 2016-05-11 ARKRAY, Inc. Zielsequenzenverstärkungsverfahren, polymorphismenerkennungsverfahren und reagenzien zur verwendung in diesen verfahren
EP2582808B1 (de) 2010-06-18 2015-04-22 Roche Diagniostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
KR101622442B1 (ko) 2010-06-18 2016-05-19 에프. 호프만-라 로슈 아게 ３’―미스매치 구별이 증가된 ｄｎａ 중합효소
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
CA2802239C (en) 2010-06-18 2016-08-23 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
EP2582807B1 (de) 2010-06-18 2015-03-18 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
US8765435B2 (en) 2011-02-15 2014-07-01 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
ITTO20110271A1 (it) * 2011-03-28 2012-09-29 Fabio Gentilini Metodo per l'identificazione di polimorfismi genetici di base singola.
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
WO2012146251A1 (en) * 2011-04-29 2012-11-01 Aarhus Universitet Method for detecting mutations using a three primer system and differently melting amplicons
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
CA2858264C (en) 2011-12-08 2018-01-02 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
EP2788479B1 (de) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
WO2013160563A1 (en) 2012-04-25 2013-10-31 Ho Tho Huu Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
US10465238B2 (en) * 2013-12-19 2019-11-05 The Board Of Trustees Of The Leland Stanford Junior University Quantification of mutant alleles and copy number variation using digital PCR with nonspecific DNA-binding dyes
WO2016193070A1 (en) 2015-05-29 2016-12-08 Roche Diagnostics Gmbh A method of inactivating microbes by citraconic anhydride
EP3768832B1 (de) 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna-polymerasen zum effizienten und effektiven einbau von methylierten dntps
WO2020053327A1 (en) 2018-09-13 2020-03-19 F. Hoffmann-La Roche Ag Mutant dna polymerase(s) with improved strand displacement ability
CN114277108B (zh) * 2021-12-13 2023-05-30 上海交通大学医学院附属第一人民医院 用于snp位点检测的引物探针组合、试剂盒和方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
GB9113223D0 (en) * 1991-06-19 1991-08-07 Oxford Instr Ltd Energy storage device
US5853989A (en) * 1991-08-27 1998-12-29 Zeneca Limited Method of characterisation of genomic DNA
ATE198358T1 (de) * 1992-04-27 2001-01-15 Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
US5981176A (en) * 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US5804380A (en) * 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
JP2004516806A (ja) * 2000-04-05 2004-06-10 モレキュラー ステージング，インコーポレイテッド プライマー伸長法を用いた対立遺伝子識別のためのプロセス
US6586183B2 (en) * 2000-04-13 2003-07-01 Genaissance Pharmaceuticals, Inc. Association of β2-adrenergic receptor haplotypes with drug response
WO2001090417A2 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
IL144515A0 (en) * 2000-07-31 2002-05-23 Pfizer Prod Inc A pcr-based multiplex assay for determining haplotype
EP1307592B1 (de) * 2000-08-11 2006-07-05 University of Utah Research Foundation Einfach markierte oligonukleotidsonden
FI20001839L (fi) * 2000-08-21 2002-02-22 Timo Korpela Hemokromatoosimutaation analyysimenetelmiä
WO2003020983A1 (en) * 2001-08-30 2003-03-13 Virginia Commonwealth University Allele specific pcr for genotyping
US7153656B2 (en) * 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
US20040121374A1 (en) * 2002-10-02 2004-06-24 Yoshihide Iwaki Method for detecting single nucleotide polymorphisms
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids

Also Published As

Publication number Publication date
JP2006230401A (ja) 2006-09-07
CA2532160A1 (en) 2006-07-28
DE602006000944D1 (de) 2008-06-05
EP1686190A1 (de) 2006-08-02
EP1686190B1 (de) 2008-04-23
US20060172324A1 (en) 2006-08-03
JP4351217B2 (ja) 2009-10-28
ES2304739T3 (es) 2008-10-16
CA2532160C (en) 2011-01-18
DE602006000944T2 (de) 2009-05-20

Similar Documents

Publication Publication Date Title
ATE393244T1 (de) Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit
WO2007130519A3 (en) Viral nucleic acid microarray and method of use
WO2007118138A3 (en) Validation of comparative genomic hybridization
RU2019138698A (ru) Способы и композиции для днк-профилирования
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2010093860A3 (en) Isolation of factors that associate directly or indirectly with chromatin
WO2010117420A3 (en) Fret-labeled compounds and uses therefor
JP2016539624A5 (de)
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
WO2010068289A3 (en) Classification of nucleic acid templates
ATE500337T1 (de) Verfahren zur durchführung homologer rekombinationen in pflanzen
WO2004071155A3 (en) Solid phase sequencing
WO2009045067A3 (en) Primers for pcr amplification comprising abasic parts within the primer sequences
WO2008033442A3 (en) Methods and compositions for performing low background multiplex nucleic acid amplification reactions
WO2005089508A3 (en) Dna sequence detection by limited primer extension
WO2007137225A3 (en) Method for indexing nucleic acid sequences for computer based searching
WO2007139723A8 (en) Chemicallymodified oligonucleotide primers for nucleic acid amplification
ATE481503T1 (de) Verfahren zur genotypisierung von antigenen von blutzellen und ein kit zur genotypisierung
CA3096478A1 (en) Highly selective nucleic acid amplification primers
WO2007115242A3 (en) Binary probes for fluorescent analysis of nucleic acids
ATE512219T1 (de) Verfahren zur sauberen klonierung
JP2013501508A5 (de)
EP2276860A4 (de) Verfahren zur identifizierung der sequenz einer oder mehrerer nukleotidvarianten in einem nukleinsäuremolekül
JP2011101652A5 (de)
WO2011115366A3 (ko) 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1686190

Country of ref document: EP