WO2011115366A3 - 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 - Google Patents
대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 Download PDFInfo
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- WO2011115366A3 WO2011115366A3 PCT/KR2011/000592 KR2011000592W WO2011115366A3 WO 2011115366 A3 WO2011115366 A3 WO 2011115366A3 KR 2011000592 W KR2011000592 W KR 2011000592W WO 2011115366 A3 WO2011115366 A3 WO 2011115366A3
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- diatoms
- species
- identification
- coast
- korea
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 한국 연안에 서식하는 규조류의 종(species)을 판별하는 방법과, 이를 위한 규조류의 종 판별용 폴리뉴클레오티드 프로브, DNA 칩 및 키트에 대한 것으로, 규조류에서 추출한 DNA에 대해 중합효소연쇄반응(PCR)을 수행하여 PCR 산물을 얻는 단계; 상기 PCR 산물을, 서열번호 3 내지 서열번호 51 중 하나 이상의 각 DNA 서열과 동일하거나 그에 상보적인(complementary) 염기서열을 각각 포함하는 프로브에 결합시키는 단계; 및, 상기 결합 여부에 따라 상기 규조류가 속하는 종을 판별하는 단계;를 포함하는 것이 특징이다. 이러한 본 발명은 다양한 규조류 종의 단염기다형성(single nucleotide polymorphism, SNPs)을 기반으로, 한국 연안에 서식하는 규조류에 대한 유전자형을 분석하고, 간단하고 신속, 정확하게 그것의 종을 판별할 수 있는 효과가 있다.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/635,887 US20130123125A1 (en) | 2010-03-19 | 2011-01-28 | Identification Method of Species of Diatoms in Coast of Korea, and Polynucleotide Probe, DNA Chip and Kit for Identification of Species of Diatoms According to Same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2010-0024939 | 2010-03-19 | ||
KR1020100024939A KR101403351B1 (ko) | 2010-03-19 | 2010-03-19 | 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2011115366A2 WO2011115366A2 (ko) | 2011-09-22 |
WO2011115366A3 true WO2011115366A3 (ko) | 2012-03-29 |
Family
ID=44649684
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/KR2011/000592 WO2011115366A2 (ko) | 2010-03-19 | 2011-01-28 | 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20130123125A1 (ko) |
KR (1) | KR101403351B1 (ko) |
WO (1) | WO2011115366A2 (ko) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102433390B (zh) * | 2012-01-09 | 2013-08-07 | 上海海洋大学 | 一种检测利玛原甲藻的方法 |
KR101687268B1 (ko) * | 2014-06-23 | 2016-12-19 | 한국해양과학기술원 | 대한민국 연안 미세조류인 디틸럼 브라이트웰리의 종 판별 방법과 이에 따른 디틸럼 브라이트웰리의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
KR101603755B1 (ko) * | 2014-06-23 | 2016-03-18 | 한국해양과학기술원 | 대한민국 연안 미세조류인 헤테로시그마 아카쉬요의 종 판별 방법과 이에 따른 헤테로시그마 아카쉬요의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
KR101602349B1 (ko) * | 2014-06-23 | 2016-03-14 | 한국해양과학기술원 | 대한민국 연안 미세조류인 아카쉬요 산구이니아의 종 판별 방법과 이에 따른아카쉬요 산구이니아의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
KR101599427B1 (ko) * | 2014-06-23 | 2016-03-04 | 한국해양과학기술원 | 대한민국 연안 미세조류인 니츠치아 임프로비사의 종 판별 방법과 이에 따른 니츠치아 임프로비사의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
KR101602346B1 (ko) * | 2014-06-23 | 2016-03-14 | 한국해양과학기술원 | 대한민국 연안 미세조류인 프로로센트럼 미니멈의 종 판별 방법과 이에 따른 프로로센트럼 미니멈의 종 판별용 폴리뉴클레오티드 프로브, dna 칩 및 키트 |
KR101706771B1 (ko) | 2015-06-12 | 2017-02-17 | 한국해양과학기술원 | 인시츄 혼성화를 사용하는 코클로디니움 폴리클리코이데스 검출방법 및 검출용 키트 |
CN106701927B (zh) * | 2016-12-09 | 2020-09-29 | 国投生物科技投资有限公司 | 一种环介导等温扩增快速检测金藻的方法及试剂盒 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5958689A (en) * | 1996-05-22 | 1999-09-28 | Monterey Bay Aquarium Research Institute | Detection of toxigenic marine diatoms of the genus Pseudo-nitzschia |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100774367B1 (ko) * | 2005-08-31 | 2007-11-08 | 인하대학교 산학협력단 | 섬모충류 특이적 프라이머 및 이를 이용한 섬모충류 검출방법 |
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2010
- 2010-03-19 KR KR1020100024939A patent/KR101403351B1/ko active IP Right Grant
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2011
- 2011-01-28 US US13/635,887 patent/US20130123125A1/en not_active Abandoned
- 2011-01-28 WO PCT/KR2011/000592 patent/WO2011115366A2/ko active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5958689A (en) * | 1996-05-22 | 1999-09-28 | Monterey Bay Aquarium Research Institute | Detection of toxigenic marine diatoms of the genus Pseudo-nitzschia |
Non-Patent Citations (4)
Title |
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EHARA, M. ET AL.: "Distribution of cognates of group II introns detected in mitochondria coxl genes of a diatom and a haptophyte", GENE, vol. 256, 3 October 2000 (2000-10-03), pages 157 - 167 * |
LIN, S. ET AL.: "High-level diversity of dinoflagellates in the Natural environment, revealed by assessment of mitochondrial coxl and cob genes for dinoflagellate DNA barcoding", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 75, no. 5, March 2009 (2009-03-01), pages 1279 - 1290 * |
MONIZ, M. B. J. ET AL.: "Barcoding diatoms: Is there a good marker?", MOLECULAR ECOLOGY RESOURCES, vol. 9, no. SUPPL., 9 May 2009 (2009-05-09), pages 65 - 74 * |
WALKER, C. E. ET AL.: "A direct immunoassay for detecting diatoms in groundwater as an indicator of the direct influence of surface water", JOURNAL OF APPLIED PHYCOLOGY., vol. 17, January 2005 (2005-01-01), pages 81 - 90 * |
Also Published As
Publication number | Publication date |
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KR101403351B1 (ko) | 2014-06-09 |
US20130123125A1 (en) | 2013-05-16 |
KR20110105669A (ko) | 2011-09-27 |
WO2011115366A2 (ko) | 2011-09-22 |
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