ATE532878T1 - Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas - Google Patents

Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas

Info

Publication number
ATE532878T1
ATE532878T1 AT06802531T AT06802531T ATE532878T1 AT E532878 T1 ATE532878 T1 AT E532878T1 AT 06802531 T AT06802531 T AT 06802531T AT 06802531 T AT06802531 T AT 06802531T AT E532878 T1 ATE532878 T1 AT E532878T1
Authority
AT
Austria
Prior art keywords
mirnas
quantifying
dna
polymorphoic
sirnas
Prior art date
Application number
AT06802531T
Other languages
English (en)
Inventor
Ruoying Tan
Caifu Chen
Karl Guegler
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE532878T1 publication Critical patent/ATE532878T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT06802531T 2005-08-24 2006-08-24 Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas ATE532878T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71148005P 2005-08-24 2005-08-24
US75030205P 2005-12-13 2005-12-13
US78331106P 2006-03-16 2006-03-16
PCT/US2006/033642 WO2007025281A2 (en) 2005-08-24 2006-08-24 A method to quantify sirnas, mirnas and polymorphic mirnas

Publications (1)

Publication Number Publication Date
ATE532878T1 true ATE532878T1 (de) 2011-11-15

Family

ID=37772526

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06802531T ATE532878T1 (de) 2005-08-24 2006-08-24 Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas

Country Status (4)

Country Link
US (4) US20070111226A1 (de)
EP (1) EP1924713B1 (de)
AT (1) ATE532878T1 (de)
WO (1) WO2007025281A2 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7642055B2 (en) * 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
EP2548962B1 (de) 2007-09-19 2016-01-13 Applied Biosystems, LLC Sirna-sequenz-unabhängige Modifikationsformate zur Verringerung von das Ziel verfehlenden phänotypischen Effekten bei RNAI und stabilisierte Formen davon
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
KR101463874B1 (ko) 2008-01-24 2014-12-05 삼성전자 주식회사 검출용 올리고머 및 이를 이용한 바이오 칩의 ｑｃ 방법
ITBO20080103A1 (it) * 2008-02-15 2009-08-16 Twof Inc Microarray per l'analisi di sequenze nucleotidiche
US20110111416A1 (en) * 2008-05-06 2011-05-12 Panagene Inc. Peptide Nucleic Acid Probes, Kits and Methods for Expression Profiling of Micrornas
EP2641976A3 (de) 2009-01-16 2014-01-08 Cepheid Verfahren zur Erkennung von Gebärmutterhalskrebs
JP5749656B2 (ja) * 2009-02-02 2015-07-15 エクシコン エ／エス 低分子ｒｎａ種の定量化のための方法
AU2010207975B2 (en) 2009-02-02 2016-04-28 Cepheid Methods of detecting sepsis
EP2628803A3 (de) 2009-02-25 2014-01-01 Cepheid Verfahren zur Erkennung von Lungenkrebs
US20120021949A1 (en) * 2009-03-31 2012-01-26 Centre Hospitalier Universitaire Vaudois Methylation Ligation-Dependent Macroarray (MLM)
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US8530170B2 (en) 2009-08-07 2013-09-10 Ohmx Corporation Enzyme triggered redox altering chemical elimination (E-trace) immunoassay
US9250234B2 (en) 2011-01-19 2016-02-02 Ohmx Corporation Enzyme triggered redox altering chemical elimination (E-TRACE) immunoassay
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
JP2013528379A (ja) 2010-05-21 2013-07-11 オームクス コーポレーション Ｐｓａ酵素活性アッセイによる癌の検出
JP5851496B2 (ja) * 2010-06-14 2016-02-03 ナショナル ユニヴァーシティー オブ シンガポール 修飾ステムループオリゴヌクレオチドが仲介する逆転写および塩基間隔が制限された定量的ｐｃｒ
CA2860739A1 (en) 2012-01-09 2013-07-18 Ohmx Corporation Enzyme cascade methods for e-trace assay signal amplification
EP2837695B1 (de) * 2012-04-12 2018-01-10 The University of Tokyo Nucleinsäurequantifizierungsverfahren, detektionssonde, detektionssondensatz und nucleinsäuredetektionsverfahren
CN103509789B (zh) * 2012-06-26 2015-12-16 舟山医院 一种用于扩增短链rna的引物及其相关方法
ES3049590T3 (en) 2012-11-02 2025-12-17 Life Technologies Corp Small rna capture, detection and quantification
US9816120B2 (en) * 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
EP2971084A1 (de) 2013-03-12 2016-01-20 Cepheid Verfahren zur krebserkennung
WO2014159073A1 (en) 2013-03-14 2014-10-02 Cepheid Methods of detecting lung cancer
EP3047274A2 (de) 2013-09-20 2016-07-27 Ohmx Corporation Psa-enzymaktivität: neuer biomarker zur bestimmung der aggressivität von prostatakrebs
US20160041118A1 (en) 2014-08-11 2016-02-11 Ohmx Corporation Enzyme triggered redox altering chemical elimination (e-trace) assay with multiplexing capabilities
CN114250278B (zh) 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
US10480023B2 (en) 2015-04-10 2019-11-19 Hudsonalpha Institute For Biotechnology Method for blocking miRNA
JP6577660B2 (ja) 2016-03-18 2019-09-18 株式会社東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
US20190284621A1 (en) * 2016-11-11 2019-09-19 Roche Innovation Center Copenhagen A/S Therapeutic oligonucleotides capture and detection
US20210269851A1 (en) * 2018-05-07 2021-09-02 Roche Innovation Center Copenhagen A/S Massively parallel discovery methods for oligonucleotide therapeutics
WO2023287669A2 (en) 2021-07-12 2023-01-19 Labsimply, Inc. Nuclease cascade assay
CN114085893B (zh) * 2021-11-16 2023-07-25 圣湘生物科技股份有限公司 一种miRNA检测的引物探针设计方法、检测组合物及试剂盒
WO2023114090A2 (en) 2021-12-13 2023-06-22 Labsimply, Inc. Signal boost cascade assay
WO2023114052A1 (en) 2021-12-13 2023-06-22 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
EP4567129A3 (de) * 2022-02-02 2025-08-13 Guardant Health, Inc. Multifunktionelle primer für gepaarte sequenzierungsablesungen
US12091689B2 (en) 2022-09-30 2024-09-17 Vedabio, Inc. Delivery of therapeutics in vivo via a CRISPR-based cascade system
WO2024076473A1 (en) 2022-10-02 2024-04-11 Vedabio, Inc. Dimerization screening assays
US11965205B1 (en) 2022-10-14 2024-04-23 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
WO2024151493A1 (en) 2023-01-10 2024-07-18 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027923A (en) * 1993-07-23 2000-02-22 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US6072923A (en) * 1996-04-30 2000-06-06 Wavefront Research, Inc. Optical switching, routing, and time delay systems using switched mirrors
JP4468488B2 (ja) * 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5968800A (en) * 1997-02-07 1999-10-19 Merck & Co., Inc. Cyclin-dependent protein kinase
EP0981609B1 (de) 1997-05-13 2001-09-19 Azign Bioscience A/S Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
US6406891B1 (en) * 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6177635B1 (en) * 1999-01-12 2001-01-23 Yazaki Corporation Wiring substrate and manufacturing method thereof
JP4426728B2 (ja) * 1999-02-24 2010-03-03 株式会社イムノキック Ｇａｎｐ蛋白質
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6777180B1 (en) * 2000-11-28 2004-08-17 Trustees Of Columbia University In The City Of New York Method for full-length cDNA cloning using degenerate stem-loop annealing primers
US7074558B2 (en) * 2001-06-07 2006-07-11 Pbi Technology, Inc. Nucleic acid amplification using an RNA polymerase and DNA/RNA mixed polymer intermediate products
US20040014058A1 (en) * 2001-10-05 2004-01-22 Alsobrook John P. Novel human proteins, polynucleotides encoding them and methods of using the same
US8090542B2 (en) * 2002-11-14 2012-01-03 Dharmacon Inc. Functional and hyperfunctional siRNA
US7851150B2 (en) * 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
ATE431433T1 (de) * 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
US8192937B2 (en) * 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules

Also Published As

Publication number Publication date
EP1924713A2 (de) 2008-05-28
US8187815B2 (en) 2012-05-29
US20120301876A1 (en) 2012-11-29
US20140377748A1 (en) 2014-12-25
WO2007025281A3 (en) 2007-11-01
US20110212494A1 (en) 2011-09-01
EP1924713B1 (de) 2011-11-09
US8710208B2 (en) 2014-04-29
WO2007025281A2 (en) 2007-03-01
EP1924713A4 (de) 2009-01-14
US20070111226A1 (en) 2007-05-17

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