ATE532878T1 - Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas - Google Patents
Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnasInfo
- Publication number
- ATE532878T1 ATE532878T1 AT06802531T AT06802531T ATE532878T1 AT E532878 T1 ATE532878 T1 AT E532878T1 AT 06802531 T AT06802531 T AT 06802531T AT 06802531 T AT06802531 T AT 06802531T AT E532878 T1 ATE532878 T1 AT E532878T1
- Authority
- AT
- Austria
- Prior art keywords
- mirnas
- quantifying
- dna
- polymorphoic
- sirnas
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108091070501 miRNA Proteins 0.000 title 2
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 102000012410 DNA Ligases Human genes 0.000 abstract 1
- 108010061982 DNA Ligases Proteins 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71148005P | 2005-08-24 | 2005-08-24 | |
| US75030205P | 2005-12-13 | 2005-12-13 | |
| US78331106P | 2006-03-16 | 2006-03-16 | |
| PCT/US2006/033642 WO2007025281A2 (en) | 2005-08-24 | 2006-08-24 | A method to quantify sirnas, mirnas and polymorphic mirnas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE532878T1 true ATE532878T1 (de) | 2011-11-15 |
Family
ID=37772526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06802531T ATE532878T1 (de) | 2005-08-24 | 2006-08-24 | Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas |
Country Status (4)
| Country | Link |
|---|---|
| US (4) | US20070111226A1 (de) |
| EP (1) | EP1924713B1 (de) |
| AT (1) | ATE532878T1 (de) |
| WO (1) | WO2007025281A2 (de) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7575863B2 (en) | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| EP1791982B1 (de) * | 2004-09-21 | 2011-01-05 | Life Technologies Corporation | Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) |
| CN101849008A (zh) | 2007-09-19 | 2010-09-29 | 应用生物系统有限公司 | 用于减少RNAi中的脱靶表型效应的siRNA的不依赖于序列的修饰形式和其稳定形式 |
| US20090181390A1 (en) * | 2008-01-11 | 2009-07-16 | Signosis, Inc. A California Corporation | High throughput detection of micrornas and use for disease diagnosis |
| KR101463874B1 (ko) * | 2008-01-24 | 2014-12-05 | 삼성전자 주식회사 | 검출용 올리고머 및 이를 이용한 바이오 칩의 qc 방법 |
| ITBO20080103A1 (it) * | 2008-02-15 | 2009-08-16 | Twof Inc | Microarray per l'analisi di sequenze nucleotidiche |
| US20110111416A1 (en) * | 2008-05-06 | 2011-05-12 | Panagene Inc. | Peptide Nucleic Acid Probes, Kits and Methods for Expression Profiling of Micrornas |
| EP2641976A3 (de) | 2009-01-16 | 2014-01-08 | Cepheid | Verfahren zur Erkennung von Gebärmutterhalskrebs |
| JP5749656B2 (ja) * | 2009-02-02 | 2015-07-15 | エクシコン エ/エス | 低分子rna種の定量化のための方法 |
| CN102388149A (zh) | 2009-02-02 | 2012-03-21 | 西菲伊德公司 | 检测败血症的方法 |
| WO2010099342A2 (en) | 2009-02-25 | 2010-09-02 | Cepheid | Methods of detecting lung cancer |
| US20120021949A1 (en) * | 2009-03-31 | 2012-01-26 | Centre Hospitalier Universitaire Vaudois | Methylation Ligation-Dependent Macroarray (MLM) |
| GB0910302D0 (en) | 2009-06-15 | 2009-07-29 | Lumora Ltd | Nucleic acid amplification |
| JP5352742B2 (ja) | 2009-08-07 | 2013-11-27 | オームクス コーポレーション | 酵素による酸化還元変化化学的脱離(e−trace)免疫学的検定法 |
| US20110117559A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| JP2013528379A (ja) | 2010-05-21 | 2013-07-11 | オームクス コーポレーション | Psa酵素活性アッセイによる癌の検出 |
| JP5851496B2 (ja) * | 2010-06-14 | 2016-02-03 | ナショナル ユニヴァーシティー オブ シンガポール | 修飾ステムループオリゴヌクレオチドが仲介する逆転写および塩基間隔が制限された定量的pcr |
| WO2012100078A1 (en) | 2011-01-19 | 2012-07-26 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) immmunoassay |
| EP2802669A1 (de) | 2012-01-09 | 2014-11-19 | Ohmx Corporation | Enzymkaskadenverfahren für eine e-trace-assay-signalverstärkung |
| EP2837695B1 (de) * | 2012-04-12 | 2018-01-10 | The University of Tokyo | Nucleinsäurequantifizierungsverfahren, detektionssonde, detektionssondensatz und nucleinsäuredetektionsverfahren |
| CN103509789B (zh) * | 2012-06-26 | 2015-12-16 | 舟山医院 | 一种用于扩增短链rna的引物及其相关方法 |
| PL3260558T3 (pl) * | 2012-11-02 | 2020-08-10 | Life Technologies Corporation | Nowe kompozycje i sposoby poprawy specyficzności pcr |
| US9816120B2 (en) | 2013-01-09 | 2017-11-14 | The Penn State Research Foundation | Low sequence bias single-stranded DNA ligation |
| WO2014164480A1 (en) | 2013-03-12 | 2014-10-09 | Cepheid | Methods of detecting cancer |
| EP2971103A1 (de) | 2013-03-14 | 2016-01-20 | Cepheid | Verfahren für den nachweis von lungenkrebs |
| WO2015042493A2 (en) | 2013-09-20 | 2015-03-26 | Ohmx Corporation | Psa enzymatic activity: a new biomarker for assessing prostate cancer aggressiveness |
| US20160041118A1 (en) | 2014-08-11 | 2016-02-11 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) assay with multiplexing capabilities |
| CN107429296B (zh) | 2015-03-13 | 2022-01-28 | 生命技术公司 | 捕获、检测和定量小rna的方法、组合物与试剂盒 |
| CA2982369A1 (en) * | 2015-04-10 | 2016-10-13 | Hudsonalpha Institute For Biotechnology | Method for blocking mirna |
| WO2017158840A1 (ja) | 2016-03-18 | 2017-09-21 | 株式会社 東芝 | 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法 |
| WO2018087200A1 (en) * | 2016-11-11 | 2018-05-17 | Roche Innovation Center Copenhagen A/S | Therapeutic oligonucleotides capture and detection |
| JP2021522816A (ja) * | 2018-05-07 | 2021-09-02 | ロシュ イノベーション センター コペンハーゲン エーエス | オリゴヌクレオチド治療のための超並列的探索方法 |
| US11639520B2 (en) | 2021-07-12 | 2023-05-02 | Labsimply, Inc. | Compositions of matter for detection assays |
| CN114085893B (zh) * | 2021-11-16 | 2023-07-25 | 圣湘生物科技股份有限公司 | 一种miRNA检测的引物探针设计方法、检测组合物及试剂盒 |
| US20230279375A1 (en) | 2021-12-13 | 2023-09-07 | Labsimply, Inc. | Signal boost cascade assay |
| WO2023114052A1 (en) * | 2021-12-13 | 2023-06-22 | Labsimply, Inc. | Tuning cascade assay kinetics via molecular design |
| EP4567129A3 (de) * | 2022-02-02 | 2025-08-13 | Guardant Health, Inc. | Multifunktionelle primer für gepaarte sequenzierungsablesungen |
| WO2024073114A1 (en) | 2022-09-30 | 2024-04-04 | Vedabio, Inc. | Delivery of therapeutics in vivo via a crispr-based cascade system |
| WO2024076473A1 (en) | 2022-10-02 | 2024-04-11 | Vedabio, Inc. | Dimerization screening assays |
| WO2024081156A1 (en) | 2022-10-14 | 2024-04-18 | Vedabio, Inc. | Detection of nucleic acid and non-nucleic acid target molecules |
| US12091690B2 (en) | 2023-01-07 | 2024-09-17 | Vedabio, Inc. | Engineered nucleic acid-guided nucleases |
| US12060602B2 (en) | 2023-01-10 | 2024-08-13 | Vedabio, Inc. | Sample splitting for multiplexed detection of nucleic acids without amplification |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027923A (en) * | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| US6072923A (en) * | 1996-04-30 | 2000-06-06 | Wavefront Research, Inc. | Optical switching, routing, and time delay systems using switched mirrors |
| CA2255774C (en) * | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5968800A (en) * | 1997-02-07 | 1999-10-19 | Merck & Co., Inc. | Cyclin-dependent protein kinase |
| ES2163271T3 (es) | 1997-05-13 | 2002-01-16 | Azign Bioscience As | Procedimiento para clonar arnm y visualizar transcriptores diferencialmente expresados (dodets). |
| US6406891B1 (en) * | 1998-09-28 | 2002-06-18 | Board Of Regents, The University Of Texas System | Dual RT procedure for cDNA synthesis |
| US6177635B1 (en) * | 1999-01-12 | 2001-01-23 | Yazaki Corporation | Wiring substrate and manufacturing method thereof |
| WO2000050611A1 (en) * | 1999-02-24 | 2000-08-31 | Sumitomo Electric Industries, Ltd. | Ganp proteins |
| US6528254B1 (en) * | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US6777180B1 (en) * | 2000-11-28 | 2004-08-17 | Trustees Of Columbia University In The City Of New York | Method for full-length cDNA cloning using degenerate stem-loop annealing primers |
| US7074558B2 (en) * | 2001-06-07 | 2006-07-11 | Pbi Technology, Inc. | Nucleic acid amplification using an RNA polymerase and DNA/RNA mixed polymer intermediate products |
| US20040014058A1 (en) * | 2001-10-05 | 2004-01-22 | Alsobrook John P. | Novel human proteins, polynucleotides encoding them and methods of using the same |
| JP2006507841A (ja) * | 2002-11-14 | 2006-03-09 | ダーマコン, インコーポレイテッド | 機能的siRNAおよび超機能的siRNA |
| US7851150B2 (en) * | 2002-12-18 | 2010-12-14 | Third Wave Technologies, Inc. | Detection of small nucleic acids |
| US7604937B2 (en) * | 2004-03-24 | 2009-10-20 | Applied Biosystems, Llc | Encoding and decoding reactions for determining target polynucleotides |
| US8192937B2 (en) * | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US20060057595A1 (en) * | 2004-09-16 | 2006-03-16 | Applera Corporation | Compositions, methods, and kits for identifying and quantitating small RNA molecules |
-
2006
- 2006-08-24 AT AT06802531T patent/ATE532878T1/de active
- 2006-08-24 US US11/467,125 patent/US20070111226A1/en not_active Abandoned
- 2006-08-24 WO PCT/US2006/033642 patent/WO2007025281A2/en not_active Ceased
- 2006-08-24 EP EP06802531A patent/EP1924713B1/de active Active
-
2011
- 2011-01-27 US US13/015,332 patent/US8187815B2/en active Active
-
2012
- 2012-04-26 US US13/456,835 patent/US8710208B2/en active Active
-
2014
- 2014-03-28 US US14/229,706 patent/US20140377748A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1924713A2 (de) | 2008-05-28 |
| US20110212494A1 (en) | 2011-09-01 |
| US20120301876A1 (en) | 2012-11-29 |
| EP1924713B1 (de) | 2011-11-09 |
| US8710208B2 (en) | 2014-04-29 |
| WO2007025281A2 (en) | 2007-03-01 |
| US8187815B2 (en) | 2012-05-29 |
| US20140377748A1 (en) | 2014-12-25 |
| US20070111226A1 (en) | 2007-05-17 |
| WO2007025281A3 (en) | 2007-11-01 |
| EP1924713A4 (de) | 2009-01-14 |
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