CN103210092B - 修饰的茎-环寡核苷酸介导的反转录和碱基间隔限制的定量pcr - Google Patents
修饰的茎-环寡核苷酸介导的反转录和碱基间隔限制的定量pcr Download PDFInfo
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- CN103210092B CN103210092B CN201180038333.8A CN201180038333A CN103210092B CN 103210092 B CN103210092 B CN 103210092B CN 201180038333 A CN201180038333 A CN 201180038333A CN 103210092 B CN103210092 B CN 103210092B
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
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Claims (18)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US35468310P | 2010-06-14 | 2010-06-14 | |
US61/354,683 | 2010-06-14 | ||
PCT/SG2011/000210 WO2011159256A1 (en) | 2010-06-14 | 2011-06-13 | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr |
Publications (2)
Publication Number | Publication Date |
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CN103210092A CN103210092A (zh) | 2013-07-17 |
CN103210092B true CN103210092B (zh) | 2015-11-25 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201180038333.8A Active CN103210092B (zh) | 2010-06-14 | 2011-06-13 | 修饰的茎-环寡核苷酸介导的反转录和碱基间隔限制的定量pcr |
Country Status (7)
Country | Link |
---|---|
US (1) | US9850527B2 (zh) |
EP (1) | EP2580355B1 (zh) |
JP (1) | JP5851496B2 (zh) |
CN (1) | CN103210092B (zh) |
HK (1) | HK1187378A1 (zh) |
SG (1) | SG185776A1 (zh) |
WO (1) | WO2011159256A1 (zh) |
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WO2011137533A1 (en) | 2010-05-05 | 2011-11-10 | The Governing Council Of The University Of Toronto | Method of processing dried samples using digital microfluidic device |
SG185776A1 (en) | 2010-06-14 | 2013-01-30 | Univ Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr |
WO2012070618A1 (ja) | 2010-11-24 | 2012-05-31 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
US9528107B2 (en) | 2012-01-31 | 2016-12-27 | Pacific Biosciences Of California, Inc. | Compositions and methods for selection of nucleic acids |
US9637781B2 (en) * | 2012-02-14 | 2017-05-02 | The Johns Hopkins University | MiRNA analysis methods |
CN103509789B (zh) * | 2012-06-26 | 2015-12-16 | 舟山医院 | 一种用于扩增短链rna的引物及其相关方法 |
WO2015076356A1 (ja) * | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖rnaの検出方法 |
GB201413717D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
GB201413718D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
CN107109474B (zh) * | 2014-08-19 | 2021-03-19 | 豪夫迈·罗氏有限公司 | 用于核酸检测的方法和组合物 |
US10695762B2 (en) | 2015-06-05 | 2020-06-30 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
EP3118313A1 (en) * | 2015-07-17 | 2017-01-18 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Cloning of single-stranded rna |
EP3325621B1 (en) * | 2015-07-17 | 2021-06-30 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Cloning of single-stranded nucleic acid |
CN105132577B (zh) * | 2015-09-30 | 2018-02-09 | 基因科技(上海)有限公司 | 一种对miRNA进行多重定量检测的方法 |
WO2018039281A1 (en) | 2016-08-22 | 2018-03-01 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
CN107868774A (zh) * | 2016-09-23 | 2018-04-03 | 上海紫众生物科技有限公司 | 一种热启动dna聚合酶、制备方法及其应用 |
EP3563151A4 (en) | 2016-12-28 | 2020-08-19 | Miroculus Inc. | DIGITAL MICROFLUIDIC DEVICES AND METHODS |
JP6448695B2 (ja) | 2017-03-21 | 2019-01-09 | 株式会社東芝 | Rna増幅方法、rna検出方法及びアッセイキット |
US11623219B2 (en) | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
CN106906211B (zh) * | 2017-04-13 | 2020-11-20 | 苏州普瑞迈德医学检验所有限公司 | 一种分子接头及其应用 |
CN110892258A (zh) | 2017-07-24 | 2020-03-17 | 米罗库鲁斯公司 | 具有集成的血浆收集设备的数字微流控系统和方法 |
CN115582155A (zh) | 2017-09-01 | 2023-01-10 | 米罗库鲁斯公司 | 数字微流控设备及其使用方法 |
CN108088826B (zh) * | 2017-12-14 | 2020-08-04 | 济南大学 | 一种检测尿嘧啶-dna糖基化酶(udg)的荧光生物传感器 |
CN112469504A (zh) | 2018-05-23 | 2021-03-09 | 米罗库鲁斯公司 | 对数字微流控中的蒸发的控制 |
CA3133124A1 (en) | 2019-04-08 | 2020-10-15 | Miroculus Inc. | Multi-cartridge digital microfluidics apparatuses and methods of use |
CN115537466A (zh) | 2019-04-30 | 2022-12-30 | 觅瑞实验室私人有限公司 | 一种检测胃癌的miRNA标志物组合及试剂盒 |
WO2021016614A1 (en) | 2019-07-25 | 2021-01-28 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
CN113005181B (zh) * | 2020-12-22 | 2022-01-18 | 广州血康陆道培生物技术有限公司 | 一种基于茎环法的多重荧光定量pcr检测非编码小rna的引物组 |
CN113151413A (zh) * | 2021-04-28 | 2021-07-23 | 杭州觅因生物科技有限公司 | 一种具有校准功能的microRNA检测试剂盒 |
WO2023023673A2 (en) | 2021-08-20 | 2023-02-23 | Kasa Bio, L.L.C. | Compositions and methods for multiplex detection of mirna and other polynucelotides |
WO2023075710A2 (en) | 2021-11-01 | 2023-05-04 | MiRXES Lab Pte. Ltd. | Circulating microrna panel for the detection of nasopharyngeal carcinoma and methods thereof |
US11857961B2 (en) | 2022-01-12 | 2024-01-02 | Miroculus Inc. | Sequencing by synthesis using mechanical compression |
CN114752665A (zh) * | 2022-05-31 | 2022-07-15 | 深圳市萨米医疗中心 | 小片段dna的检测方法及检测试剂盒 |
Citations (1)
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US6027891A (en) * | 1995-02-24 | 2000-02-22 | Deutsches Krebsforschungszentrum Des Offentlichen Rechts | Method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV |
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US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
CA2433330A1 (en) | 2000-12-27 | 2002-07-25 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
US7273730B2 (en) * | 2002-05-24 | 2007-09-25 | Invitrogen Corporation | Nested PCR employing degradable primers |
EP1537240A2 (en) | 2002-09-06 | 2005-06-08 | Johns Hopkins Singapore Pte Ltd | Strand specific detection and quantification |
AU2002951347A0 (en) | 2002-09-09 | 2002-09-26 | Benitec Australia Ltd | Genetic silencing |
US20070054287A1 (en) | 2005-05-31 | 2007-03-08 | Applera Corporation | Method for identifying medically important cell populations using micro rna as tissue specific biomarkers |
US20070077570A1 (en) * | 2005-05-31 | 2007-04-05 | Applera Corporation | Multiplexed amplification of short nucleic acids |
DK1924704T3 (da) * | 2005-08-02 | 2011-09-05 | Rubicon Genomics Inc | Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion |
EP1924713B1 (en) * | 2005-08-24 | 2011-11-09 | Life Technologies Corporation | A method to quantify sirnas, mirnas and polymorphic mirnas |
DK2279263T3 (da) | 2008-04-30 | 2013-11-11 | Integrated Dna Tech Inc | Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer |
WO2010120853A2 (en) * | 2009-04-16 | 2010-10-21 | Padma Arunachalam | Methods and compositions to detect and differentiate small rnas in rna maturation pathway |
US9587270B2 (en) * | 2009-06-29 | 2017-03-07 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
SG185776A1 (en) | 2010-06-14 | 2013-01-30 | Univ Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr |
-
2011
- 2011-06-13 SG SG2012087144A patent/SG185776A1/en unknown
- 2011-06-13 US US13/704,482 patent/US9850527B2/en active Active
- 2011-06-13 EP EP11796068.2A patent/EP2580355B1/en active Active
- 2011-06-13 JP JP2013515301A patent/JP5851496B2/ja active Active
- 2011-06-13 CN CN201180038333.8A patent/CN103210092B/zh active Active
- 2011-06-13 WO PCT/SG2011/000210 patent/WO2011159256A1/en active Application Filing
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2014
- 2014-01-10 HK HK14100278.7A patent/HK1187378A1/zh unknown
Patent Citations (1)
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US6027891A (en) * | 1995-02-24 | 2000-02-22 | Deutsches Krebsforschungszentrum Des Offentlichen Rechts | Method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV |
Non-Patent Citations (1)
Title |
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High-performance quantification of mature microRNAs by real-time RT-PCR using deoxyuridine-incorporated oligonucleotides and hemi-nested primers;Guoqiang Wan, et al.;《RNA》;20100731;第16卷;1436–1445 * |
Also Published As
Publication number | Publication date |
---|---|
EP2580355A4 (en) | 2013-11-20 |
HK1187378A1 (zh) | 2014-04-04 |
EP2580355B1 (en) | 2018-03-07 |
WO2011159256A1 (en) | 2011-12-22 |
SG185776A1 (en) | 2013-01-30 |
EP2580355A1 (en) | 2013-04-17 |
CN103210092A (zh) | 2013-07-17 |
JP2013530698A (ja) | 2013-08-01 |
US20130177915A1 (en) | 2013-07-11 |
US9850527B2 (en) | 2017-12-26 |
JP5851496B2 (ja) | 2016-02-03 |
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Application publication date: 20130717 Assignee: Hangzhou miyin Biotechnology Co.,Ltd. Assignor: Meirui Private Ltd. Contract record no.: X2022990000090 Denomination of invention: Modified stem loop oligonucleotide mediated reverse transcription and base interval limited quantitative PCR Granted publication date: 20151125 License type: Common License Record date: 20220218 |