HK1187378A1 - 修飾的莖-環寡核苷酸介導的反轉錄和碱基間隔限制的定量 - Google Patents

修飾的莖-環寡核苷酸介導的反轉錄和碱基間隔限制的定量

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Publication number
HK1187378A1
HK1187378A1 HK14100278.7A HK14100278A HK1187378A1 HK 1187378 A1 HK1187378 A1 HK 1187378A1 HK 14100278 A HK14100278 A HK 14100278A HK 1187378 A1 HK1187378 A1 HK 1187378A1
Authority
HK
Hong Kong
Prior art keywords
pcr
base
reverse transcription
modified stem
oligonucleotide mediated
Prior art date
Application number
HK14100278.7A
Other languages
English (en)
Inventor
恒方．涂
阿茲琳達．
．安瓦爾
Original Assignee
新加坡國立大學
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 新加坡國立大學 filed Critical 新加坡國立大學
Publication of HK1187378A1 publication Critical patent/HK1187378A1/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK14100278.7A 2010-06-14 2014-01-10 修飾的莖-環寡核苷酸介導的反轉錄和碱基間隔限制的定量 HK1187378A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35468310P 2010-06-14 2010-06-14
PCT/SG2011/000210 WO2011159256A1 (en) 2010-06-14 2011-06-13 Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr

Publications (1)

Publication Number Publication Date
HK1187378A1 true HK1187378A1 (zh) 2014-04-04

Family

ID=45348446

Family Applications (1)

Application Number Title Priority Date Filing Date
HK14100278.7A HK1187378A1 (zh) 2010-06-14 2014-01-10 修飾的莖-環寡核苷酸介導的反轉錄和碱基間隔限制的定量

Country Status (7)

Country Link
US (1) US9850527B2 (zh)
EP (1) EP2580355B1 (zh)
JP (1) JP5851496B2 (zh)
CN (1) CN103210092B (zh)
HK (1) HK1187378A1 (zh)
SG (1) SG185776A1 (zh)
WO (1) WO2011159256A1 (zh)

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SG185776A1 (en) 2010-06-14 2013-01-30 Univ Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr
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US9637781B2 (en) * 2012-02-14 2017-05-02 The Johns Hopkins University MiRNA analysis methods
CN103509789B (zh) * 2012-06-26 2015-12-16 舟山医院 一种用于扩增短链rna的引物及其相关方法
WO2015076356A1 (ja) * 2013-11-22 2015-05-28 株式会社カネカ 短鎖ｒｎａの検出方法
GB201413717D0 (en) 2014-08-01 2014-09-17 Olink Ab Method for selecting a target nucleic acid sequence
GB201413718D0 (en) 2014-08-01 2014-09-17 Olink Ab Method for selecting a target nucleic acid sequence
CN107109474B (zh) * 2014-08-19 2021-03-19 豪夫迈·罗氏有限公司 用于核酸检测的方法和组合物
US10695762B2 (en) 2015-06-05 2020-06-30 Miroculus Inc. Evaporation management in digital microfluidic devices
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
EP3118313A1 (en) * 2015-07-17 2017-01-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Cloning of single-stranded rna
EP3325621B1 (en) * 2015-07-17 2021-06-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Cloning of single-stranded nucleic acid
CN105132577B (zh) * 2015-09-30 2018-02-09 基因科技（上海）有限公司 一种对miRNA进行多重定量检测的方法
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
CN107868774A (zh) * 2016-09-23 2018-04-03 上海紫众生物科技有限公司 一种热启动dna聚合酶、制备方法及其应用
EP3563151A4 (en) 2016-12-28 2020-08-19 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS
JP6448695B2 (ja) 2017-03-21 2019-01-09 株式会社東芝 Ｒｎａ増幅方法、ｒｎａ検出方法及びアッセイキット
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
CN106906211B (zh) * 2017-04-13 2020-11-20 苏州普瑞迈德医学检验所有限公司 一种分子接头及其应用
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
CN115582155A (zh) 2017-09-01 2023-01-10 米罗库鲁斯公司 数字微流控设备及其使用方法
CN108088826B (zh) * 2017-12-14 2020-08-04 济南大学 一种检测尿嘧啶-dna糖基化酶（udg）的荧光生物传感器
CN112469504A (zh) 2018-05-23 2021-03-09 米罗库鲁斯公司 对数字微流控中的蒸发的控制
CA3133124A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
CN115537466A (zh) 2019-04-30 2022-12-30 觅瑞实验室私人有限公司 一种检测胃癌的miRNA标志物组合及试剂盒
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN113005181B (zh) * 2020-12-22 2022-01-18 广州血康陆道培生物技术有限公司 一种基于茎环法的多重荧光定量pcr检测非编码小rna的引物组
CN113151413A (zh) * 2021-04-28 2021-07-23 杭州觅因生物科技有限公司 一种具有校准功能的microRNA检测试剂盒
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Also Published As

Publication number Publication date
EP2580355A4 (en) 2013-11-20
EP2580355B1 (en) 2018-03-07
WO2011159256A1 (en) 2011-12-22
SG185776A1 (en) 2013-01-30
EP2580355A1 (en) 2013-04-17
CN103210092A (zh) 2013-07-17
CN103210092B (zh) 2015-11-25
JP2013530698A (ja) 2013-08-01
US20130177915A1 (en) 2013-07-11
US9850527B2 (en) 2017-12-26
JP5851496B2 (ja) 2016-02-03

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