DE60331072D1 - Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität - Google Patents

Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität

Info

Publication number
DE60331072D1
DE60331072D1 DE60331072T DE60331072T DE60331072D1 DE 60331072 D1 DE60331072 D1 DE 60331072D1 DE 60331072 T DE60331072 T DE 60331072T DE 60331072 T DE60331072 T DE 60331072T DE 60331072 D1 DE60331072 D1 DE 60331072D1
Authority
DE
Germany
Prior art keywords
dna
nucleic acids
low complexity
disclosed
treated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60331072T
Other languages
English (en)
Inventor
Tamas Rujan
Armin Schmitt
Peter Adorjan
Christian Piepenbrock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Application granted granted Critical
Publication of DE60331072D1 publication Critical patent/DE60331072D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60331072T 2002-08-02 2003-08-01 Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität Expired - Lifetime DE60331072D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10236406A DE10236406C1 (de) 2002-08-02 2002-08-02 Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
PCT/EP2003/008602 WO2004015139A1 (en) 2002-08-02 2003-08-01 Method for amplification of nucleic acids of low complexity

Publications (1)

Publication Number Publication Date
DE60331072D1 true DE60331072D1 (de) 2010-03-11

Family

ID=29594625

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10236406A Expired - Fee Related DE10236406C1 (de) 2002-08-02 2002-08-02 Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
DE60331072T Expired - Lifetime DE60331072D1 (de) 2002-08-02 2003-08-01 Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10236406A Expired - Fee Related DE10236406C1 (de) 2002-08-02 2002-08-02 Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität

Country Status (6)

Country Link
US (1) US20070178453A1 (de)
EP (1) EP1525328B1 (de)
AT (1) ATE455866T1 (de)
AU (1) AU2003266255A1 (de)
DE (2) DE10236406C1 (de)
WO (1) WO2004015139A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
CA2537810C (en) 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)
EP1828411B1 (de) * 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Verfahren zur vereinfachung mikrobieller nukleinsäuren durch chemische modifikation von cytosinen
AU2005312354B2 (en) * 2004-12-03 2007-05-03 Human Genetic Signatures Pty Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2007019670A1 (en) * 2005-07-01 2007-02-22 Graham, Robert Method and nucleic acids for the improved treatment of breast cancers
JP2009538603A (ja) * 2006-06-02 2009-11-12 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 微生物の検出および分析に使用するための微生物改変核酸
MY167564A (en) 2011-09-07 2018-09-14 Human Genetic Signatures Pty Ltd Molecular detection assay
CA2983819A1 (en) * 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CN111183229A (zh) 2017-08-11 2020-05-19 阿提拉生物系统股份有限公司 使用有限核苷酸组成的引物的数字扩增

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6007231A (en) * 1996-08-14 1999-12-28 Academy Of Applied Science Method of computer aided automated diagnostic DNA test design, and apparatus therefor
WO1998056952A1 (en) 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6365376B1 (en) * 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
GB9929720D0 (en) 1999-12-17 2000-02-09 Zeneca Ltd Diagnostic method
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
US6898531B2 (en) * 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
DE10160983B4 (de) * 2001-12-05 2004-12-09 Epigenomics Ag Verfahren und Integrierte Vorrichtung zum Nachweis von Cytosinmethylierungen
DE10201138B4 (de) * 2002-01-08 2005-03-10 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)

Also Published As

Publication number Publication date
US20070178453A1 (en) 2007-08-02
EP1525328A1 (de) 2005-04-27
ATE455866T1 (de) 2010-02-15
AU2003266255A1 (en) 2004-02-25
EP1525328B1 (de) 2010-01-20
DE10236406C1 (de) 2003-12-24
WO2004015139A1 (en) 2004-02-19

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