DE10201138B4 - Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) - Google Patents

Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) Download PDF

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Publication number
DE10201138B4
DE10201138B4 DE10201138A DE10201138A DE10201138B4 DE 10201138 B4 DE10201138 B4 DE 10201138B4 DE 10201138 A DE10201138 A DE 10201138A DE 10201138 A DE10201138 A DE 10201138A DE 10201138 B4 DE10201138 B4 DE 10201138B4
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DE
Germany
Prior art keywords
mla
ligation
exponential
detection
methylation patterns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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DE10201138A
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English (en)
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DE10201138A1 (de
Inventor
Kurt Berlin
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Epigenomics AG
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Epigenomics AG
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Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to DE10201138A priority Critical patent/DE10201138B4/de
Priority to JP2003558202A priority patent/JP2005514035A/ja
Priority to US10/501,040 priority patent/US20050064428A1/en
Priority to AU2003206620A priority patent/AU2003206620B2/en
Priority to PCT/DE2003/000073 priority patent/WO2003057909A2/de
Priority to EP03704206A priority patent/EP1463841A2/de
Publication of DE10201138A1 publication Critical patent/DE10201138A1/de
Application granted granted Critical
Publication of DE10201138B4 publication Critical patent/DE10201138B4/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
DE10201138A 2002-01-08 2002-01-08 Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) Expired - Fee Related DE10201138B4 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE10201138A DE10201138B4 (de) 2002-01-08 2002-01-08 Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
JP2003558202A JP2005514035A (ja) 2002-01-08 2003-01-08 ハイブリダイゼーションしたプローブオリゴヌクレオチド（ｍｌａ）の指数的ライゲーションによるシトシン−メチル化パターンの検出方法
US10/501,040 US20050064428A1 (en) 2002-01-08 2003-01-08 Method for detecting cytosine-methylation patterns by exponential ligation of hybridised probe oligo-nucleotides (mla)
AU2003206620A AU2003206620B2 (en) 2002-01-08 2003-01-08 Method for detecting cytosine-methylation patterns by exponential ligation of hybridised probe oligo-nucleotides (MLA)
PCT/DE2003/000073 WO2003057909A2 (de) 2002-01-08 2003-01-08 Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla)
EP03704206A EP1463841A2 (de) 2002-01-08 2003-01-08 Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10201138A DE10201138B4 (de) 2002-01-08 2002-01-08 Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)

Publications (2)

Publication Number Publication Date
DE10201138A1 DE10201138A1 (de) 2003-07-17
DE10201138B4 true DE10201138B4 (de) 2005-03-10

Family

ID=7712097

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10201138A Expired - Fee Related DE10201138B4 (de) 2002-01-08 2002-01-08 Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)

Country Status (6)

Country Link
US (1) US20050064428A1 (de)
EP (1) EP1463841A2 (de)
JP (1) JP2005514035A (de)
AU (1) AU2003206620B2 (de)
DE (1) DE10201138B4 (de)
WO (1) WO2003057909A2 (de)

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DE19951189C2 (de) * 1999-10-15 2003-11-06 Epigenomics Ag Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
CA2507189C (en) * 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
AU2004235331B2 (en) * 2003-04-25 2008-12-18 Sequenom, Inc. Fragmentation-based methods and systems for De Novo sequencing
WO2005024068A2 (en) * 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
CA2561381C (en) 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US7608394B2 (en) * 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
JPWO2005100240A1 (ja) * 2004-04-08 2008-03-06 東洋紡績株式会社 Ｄｎａの脱アミノ化のための組成物及びメチル化ｄｎａの検出方法
US7364855B2 (en) 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
WO2006031745A2 (en) * 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20070196849A1 (en) * 2006-02-22 2007-08-23 Applera Corporation Double-ligation Method for Haplotype and Large-scale Polymorphism Detection
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2008091913A2 (en) 2007-01-23 2008-07-31 Sequenom, Inc. Method for accurate assessment of dna quality after bisulfite treatment
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
AU2009223671B2 (en) * 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20110143959A1 (en) * 2008-08-13 2011-06-16 Rosetta Genomics Ltd. Compositions and methods for determining the prognosis of bladder urothelial cancer
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
ES2534200T3 (es) 2009-08-03 2015-04-20 Epigenomics Ag Métodos para la preservación de la complejidad de la secuencia del ADN genómico
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
EP2966179B1 (de) * 2013-03-07 2019-05-08 Sekisui Medical Co., Ltd. Verfahren zum nachweis methylierter dna
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
EP3117011B1 (de) 2014-03-13 2020-05-06 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
JP7362901B2 (ja) 2020-03-25 2023-10-17 富士フイルム株式会社 塩基のメチル化度の算出方法及びプログラム

Citations (2)

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Publication number Priority date Publication date Assignee Title
DE10010281A1 (de) * 2000-02-25 2001-09-06 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10029915A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen

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US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10010281A1 (de) * 2000-02-25 2001-09-06 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10029915A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen

Also Published As

Publication number Publication date
JP2005514035A (ja) 2005-05-19
AU2003206620B2 (en) 2007-08-09
DE10201138A1 (de) 2003-07-17
US20050064428A1 (en) 2005-03-24
WO2003057909A3 (de) 2003-10-16
AU2003206620A1 (en) 2003-07-24
EP1463841A2 (de) 2004-10-06
WO2003057909A2 (de) 2003-07-17

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OP8 Request for examination as to paragraph 44 patent law
8364 No opposition during term of opposition
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20110802