DE10253337B4 - Verfahren zum Nachweis einer Nukleinsäure - Google Patents

Verfahren zum Nachweis einer Nukleinsäure Download PDF

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Publication number
DE10253337B4
DE10253337B4 DE10253337A DE10253337A DE10253337B4 DE 10253337 B4 DE10253337 B4 DE 10253337B4 DE 10253337 A DE10253337 A DE 10253337A DE 10253337 A DE10253337 A DE 10253337A DE 10253337 B4 DE10253337 B4 DE 10253337B4
Authority
DE
Germany
Prior art keywords
detecting
nucleic acid
nucleic
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE10253337A
Other languages
English (en)
Other versions
DE10253337A1 (de
Inventor
Roland Barten
Dirk Kuhlmeier
Anja Weiland
Hans Kosak
Joerg Hassmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority to DE10253337A priority Critical patent/DE10253337B4/de
Priority to PCT/EP2003/012694 priority patent/WO2004044240A2/de
Priority to AU2003292013A priority patent/AU2003292013A1/en
Priority to US10/534,711 priority patent/US20060024674A1/en
Publication of DE10253337A1 publication Critical patent/DE10253337A1/de
Application granted granted Critical
Publication of DE10253337B4 publication Critical patent/DE10253337B4/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE10253337A 2002-11-14 2002-11-14 Verfahren zum Nachweis einer Nukleinsäure Expired - Fee Related DE10253337B4 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE10253337A DE10253337B4 (de) 2002-11-14 2002-11-14 Verfahren zum Nachweis einer Nukleinsäure
PCT/EP2003/012694 WO2004044240A2 (de) 2002-11-14 2003-11-13 Verfahren zum parallelen nachweis unterschiedlicher nukleinsäuren
AU2003292013A AU2003292013A1 (en) 2002-11-14 2003-11-13 Method for conducting the parallel identification of different nucleic acids
US10/534,711 US20060024674A1 (en) 2002-11-14 2003-11-13 Method for conducting the parallel identification of different nucleic acids

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10253337A DE10253337B4 (de) 2002-11-14 2002-11-14 Verfahren zum Nachweis einer Nukleinsäure

Publications (2)

Publication Number Publication Date
DE10253337A1 DE10253337A1 (de) 2004-06-03
DE10253337B4 true DE10253337B4 (de) 2005-10-20

Family

ID=32240087

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10253337A Expired - Fee Related DE10253337B4 (de) 2002-11-14 2002-11-14 Verfahren zum Nachweis einer Nukleinsäure

Country Status (4)

Country Link
US (1) US20060024674A1 (de)
AU (1) AU2003292013A1 (de)
DE (1) DE10253337B4 (de)
WO (1) WO2004044240A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7695941B2 (en) * 2005-06-16 2010-04-13 The United States Of America As Represented By The Secretary Of The Navy Multiplexed polymerase chain reaction for genetic sequence analysis
TWI495727B (zh) * 2010-10-08 2015-08-11 Nat Applied Res Laboratories 微型電化學多重即時定量聚合酶連鎖反應(ｐｃｒ)系統
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
CN107429292B (zh) 2014-12-15 2021-12-17 塞弗德公司 底数大于2的指数核酸扩增
US10283531B2 (en) * 2016-07-29 2019-05-07 Lg Display Co., Ltd. Thin film transistor, method for manufacturing the same, and display device including the same
EP3378950A1 (de) * 2017-03-21 2018-09-26 Sequencing Multiplex SLK Einfache einschritt-amplifikation und -markierung (eosal)
WO2020092134A1 (en) 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
DE19934084A1 (de) * 1999-07-15 2001-01-18 Universitaetsklinikum Charite Verfahren und Testbesteck zur Markierung von DNA-Fragmenten während einer PCR-Reaktion

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
EP1193315A1 (de) * 1995-06-27 2002-04-03 University of North Carolina at Chapel Hill et al. Mikroelektronische Anordnung zur elektrochemischen Detektion
US6180346B1 (en) * 1995-06-27 2001-01-30 The Universtiy Of North Carolina At Chapel Hill Electropolymerizable film, and method of making and use thereof
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
AU3984699A (en) * 1998-05-12 1999-11-29 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
GB9902971D0 (en) * 1999-02-11 1999-03-31 Zeneca Ltd Assay
GB0019179D0 (en) * 2000-08-07 2000-09-27 Potter Colin G Genetic analysis with a universal probe system
DE10046184A1 (de) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz
WO2002059353A2 (en) * 2001-01-26 2002-08-01 Bio S & T Two-step amplification using a program primer followed by specific primers
WO2003060159A2 (en) * 2002-01-15 2003-07-24 Matforsk Methods of nucleic acid amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
DE19934084A1 (de) * 1999-07-15 2001-01-18 Universitaetsklinikum Charite Verfahren und Testbesteck zur Markierung von DNA-Fragmenten während einer PCR-Reaktion

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JENISON, R. u.a.: Use of a thin film biosensor forrapid visual detection of PCR products in a multi-plex format. Biosens. Bioelectron. (2001) 16(9-12)757-63 *

Also Published As

Publication number Publication date
DE10253337A1 (de) 2004-06-03
WO2004044240A3 (de) 2004-07-29
WO2004044240A2 (de) 2004-05-27
US20060024674A1 (en) 2006-02-02
AU2003292013A1 (en) 2004-06-03

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
8364 No opposition during term of opposition
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20130601