DE10083729D2 - Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA - Google Patents

Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA

Info

Publication number
DE10083729D2
DE10083729D2 DE10083729T DE10083729T DE10083729D2 DE 10083729 D2 DE10083729 D2 DE 10083729D2 DE 10083729 T DE10083729 T DE 10083729T DE 10083729 T DE10083729 T DE 10083729T DE 10083729 D2 DE10083729 D2 DE 10083729D2
Authority
DE
Germany
Prior art keywords
genomic dna
methylation state
parallel detection
detection
parallel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE10083729T
Other languages
English (en)
Inventor
Alexander Olek
Christian Piepenbrock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to DE10083729T priority Critical patent/DE10083729D2/de
Application granted granted Critical
Publication of DE10083729D2 publication Critical patent/DE10083729D2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE10083729T 1999-12-06 2000-12-06 Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA Expired - Lifetime DE10083729D2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE10083729T DE10083729D2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19959691A DE19959691A1 (de) 1999-12-06 1999-12-06 Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
DE10083729T DE10083729D2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA
PCT/DE2000/004381 WO2001042493A2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna

Publications (1)

Publication Number Publication Date
DE10083729D2 true DE10083729D2 (de) 2003-05-15

Family

ID=7932213

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19959691A Withdrawn DE19959691A1 (de) 1999-12-06 1999-12-06 Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
DE10083729T Expired - Lifetime DE10083729D2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19959691A Withdrawn DE19959691A1 (de) 1999-12-06 1999-12-06 Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA

Country Status (6)

Country Link
US (1) US20040248090A1 (de)
EP (1) EP1238112A2 (de)
AU (1) AU778411B2 (de)
CA (1) CA2395047A1 (de)
DE (2) DE19959691A1 (de)
WO (1) WO2001042493A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
AUPR142500A0 (en) * 2000-11-13 2000-12-07 Human Genetic Signatures Pty Ltd A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences
DE10151055B4 (de) 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2004050915A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Determination of methylation of nucleic acid sequences
ATE476522T1 (de) * 2003-03-13 2010-08-15 Beijing Inst Cancer Res Verfahren zur in-vitro-erkennung von anormaler magen-dysplasie
WO2004111266A1 (en) 2003-06-17 2004-12-23 Human Genetic Signatures Pty Ltd Methods for genome amplification
JP4714148B2 (ja) 2003-09-04 2011-06-29 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 核酸検出アッセイ
US7365058B2 (en) 2004-04-13 2008-04-29 The Rockefeller University MicroRNA and methods for inhibiting same
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DK1794173T3 (da) 2004-09-10 2010-10-25 Human Genetic Signatures Pty Amplifikationsblokker indeholdende interkalaterende nukleinsyrer (INA) indeholdende interkalaterende pseudonukleotider (IPN)
JP5116481B2 (ja) 2004-12-03 2013-01-09 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド シトシンの化学修飾により微生物核酸を簡素化するための方法
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
JP2008541705A (ja) 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2007030882A1 (en) 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for a health state
US8119788B2 (en) * 2005-09-27 2012-02-21 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Compositions and methods for the detection of Candida species
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
CN101918595A (zh) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 用于扩增和复制亚硫酸氢盐修饰的核酸的酶
US8361746B2 (en) * 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
EP2322656A1 (de) * 2009-11-17 2011-05-18 Centre National de la Recherche Scientifique (C.N.R.S) Verfahren zur Diagnose von Hautkrankheiten
KR101912488B1 (ko) 2011-09-07 2018-10-26 휴먼 제네틱 시그너처스 피티와이 엘티디 분자 검출 분석법
EP2971171A4 (de) * 2013-03-14 2016-11-02 Abbott Molecular Inc Multiplex-methylierungs-spezifische verstärkersysteme und -verfahren

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19543065C2 (de) * 1995-11-09 2002-10-31 Gag Bioscience Gmbh Zentrum Fu Verfahren zur Genomanalyse und Mittel zur Durchführung des Verfahrens
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
AUPP312998A0 (en) * 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay

Also Published As

Publication number Publication date
WO2001042493A3 (de) 2002-02-07
CA2395047A1 (en) 2001-06-14
EP1238112A2 (de) 2002-09-11
AU778411B2 (en) 2004-12-02
US20040248090A1 (en) 2004-12-09
AU2663201A (en) 2001-06-18
DE19959691A1 (de) 2001-08-16
WO2001042493A2 (de) 2001-06-14

Similar Documents

Publication Publication Date Title
DE10083729D2 (de) Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA
DE10010281B4 (de) Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE60029876D1 (de) Verfahren und Oligonukleotide zum Nachweis von Nukleinsäuresequenzvariationen
DE69929099D1 (de) Gerät zur Extraktion von Nukleinsäuren
DE10084502T1 (de) Verfahren zur Reinigung von Nukleinsäure mit Siliciumcarbid
DE60139275D1 (de) Verfahren zur detektion von unbeweglichen objekten
DE60223299D1 (de) Verfahren zur Sequenzierung von Nukleinsäuren
DE69916400D1 (de) Verfahren zur selektiven Entfernung von Nitrilen
DE50000780D1 (de) Vorrichtung zur Detektion von Objekten
DE60012549D1 (de) Verfahren zur isolierung von nukleinsäuren und kit
DE60038975D1 (de) Verfahren zur reinigung von bishydroxyalkylterephthalaten
IL138229A0 (en) Method and system for detecting nucleic acids
DE60030811D1 (de) Verfahren zur Ampifizierung von RNA
DE50203409D1 (de) Verfahren zur branderkennung
DE60144361D1 (de) Verfahren zur Reinigung von Vinylpolymeren
AU2002363259A1 (en) Method for the detection of cytosine methylation in immobilised DNA samples
DE60024535D1 (de) Verfahren zur Zeichentrennung
DE50204680D1 (de) Verfahren zur detektion von nukleinsäuremolekülen
DE60141221D1 (de) Verfahren zur genexpressionsanalyse
DE60008926D1 (de) Verfahren zur Weinstabilisierung
DE60135751D1 (de) Methode zum nachweis von nukleinsäurepolymorphismen
DE50003545D1 (de) Verfahren zur entschwefelung
DE60024356D1 (de) Verfahren zur selektiven Entfernung von Kohlenmonoxid
DE60033734D1 (de) Verfahren zur selektiven Entfernung von Kohlenmonoxid
DE60324819D1 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus

Legal Events

Date Code Title Description
8139 Disposal/non-payment of the annual fee
8166 Unexamined publication of following international application in german language revoked
8170 Reinstatement of the former position