DE60012549D1 - Verfahren zur isolierung von nukleinsäuren und kit - Google Patents

Verfahren zur isolierung von nukleinsäuren und kit

Info

Publication number
DE60012549D1
DE60012549D1 DE60012549T DE60012549T DE60012549D1 DE 60012549 D1 DE60012549 D1 DE 60012549D1 DE 60012549 T DE60012549 T DE 60012549T DE 60012549 T DE60012549 T DE 60012549T DE 60012549 D1 DE60012549 D1 DE 60012549D1
Authority
DE
Germany
Prior art keywords
kit
nucleic acids
isolating nucleic
isolating
acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60012549T
Other languages
English (en)
Other versions
DE60012549T2 (de
Inventor
C Haddad
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3M Innovative Properties Co
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Application granted granted Critical
Publication of DE60012549D1 publication Critical patent/DE60012549D1/de
Publication of DE60012549T2 publication Critical patent/DE60012549T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00Nanotechnology
    • Y10S977/902Specified use of nanostructure
    • Y10S977/904Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
DE60012549T 1999-09-21 2000-01-28 Verfahren zur isolierung von nukleinsäuren und kit Expired - Lifetime DE60012549T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US400037 1999-09-21
US09/400,037 US6383783B1 (en) 1999-09-21 1999-09-21 Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
PCT/US2000/002260 WO2001021632A1 (en) 1999-09-21 2000-01-28 Method for nucleic acid isolation and kit

Publications (2)

Publication Number Publication Date
DE60012549D1 true DE60012549D1 (de) 2004-09-02
DE60012549T2 DE60012549T2 (de) 2005-09-01

Family

ID=23581969

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60012549T Expired - Lifetime DE60012549T2 (de) 1999-09-21 2000-01-28 Verfahren zur isolierung von nukleinsäuren und kit

Country Status (6)

Country Link
US (2) US6383783B1 (de)
EP (1) EP1216255B1 (de)
JP (1) JP2003510254A (de)
AU (1) AU2745000A (de)
DE (1) DE60012549T2 (de)
WO (1) WO2001021632A1 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
WO2001057247A2 (de) * 2000-02-04 2001-08-09 Qiagen Gmbh Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
FR2808276B1 (fr) * 2000-04-26 2004-04-02 Renaud Nalin Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'etre obtenu par ledit procede
DE60125406T2 (de) * 2000-10-24 2007-10-11 Kaneka Corp. Hydrophilierte membran und hydrophilierungsverfahren dafür
US7347976B2 (en) * 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US20040067495A1 (en) * 2002-10-08 2004-04-08 Ray Gustav Allen Polynucleic acid and polyamino acid binding substrate
US7981600B2 (en) * 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
US7031802B2 (en) * 2003-08-13 2006-04-18 Hewlett-Packard Development Company, L.P. Semi-autonomous operation of a robotic device
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
DE10344820B4 (de) * 2003-09-26 2009-04-16 Sartorius Stedim Biotech Gmbh Adsorptionsmembranen, Verfahren zur Herstellung derselben und Verwendung der Adsorptionsmembranen in Vorrichtungen
DE10344819B4 (de) * 2003-09-26 2017-06-29 Sartorius Stedim Biotech Gmbh Adsorptionsmembranen, Verfahren zur Herstellung derselben und Vorrichtungen, welche die Adsorptionsmembranen umfassen
US20080161549A1 (en) * 2003-10-24 2008-07-03 Rosetta Inpharmatics Llc Method of purifying nucleic acid molecules using proteinase k
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7727710B2 (en) * 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US7939249B2 (en) * 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
WO2007094945A2 (en) * 2006-02-09 2007-08-23 Waters Investments Limited Mobil bead configuration immunoaffinity column and methods of use
KR100785010B1 (ko) * 2006-04-06 2007-12-11 삼성전자주식회사 수소 결합을 이용하여 고체 지지체의 친수성 표면 상에서핵산 정제 방법 및 장치
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
US20100129878A1 (en) * 2007-04-25 2010-05-27 Parthasarathy Ranjani V Methods for nucleic acid amplification
WO2016130553A1 (en) * 2015-02-13 2016-08-18 Siemens Healthcare Diagnostics Inc. Methods and apparatus providing reduced carryover during pipetting operations
EP3696275A4 (de) * 2017-10-11 2021-05-26 MGI Tech Co., Ltd. Verfahren zur verbesserung der belastung und stabilität von nukleinsäuren auf einem festen träger

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4153661A (en) 1977-08-25 1979-05-08 Minnesota Mining And Manufacturing Company Method of making polytetrafluoroethylene composite sheet
US4460642A (en) 1981-06-26 1984-07-17 Minnesota Mining And Manufacturing Company Water-swellable composite sheet of microfibers of PTFE and hydrophilic absorptive particles
US4565663A (en) 1981-06-26 1986-01-21 Minnesota Mining And Manufacturing Company Method for making water-swellable composite sheet
US4373519A (en) 1981-06-26 1983-02-15 Minnesota Mining And Manufacturing Company Composite wound dressing
US4483920A (en) 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
US4539256A (en) 1982-09-09 1985-09-03 Minnesota Mining And Manufacturing Co. Microporous sheet material, method of making and articles made therewith
US4726989A (en) 1986-12-11 1988-02-23 Minnesota Mining And Manufacturing Microporous materials incorporating a nucleating agent and methods for making same
JPH0611747B2 (ja) 1987-11-19 1994-02-16 大正製薬株式会社 スルホンアニリド化合物
US4882147A (en) 1987-12-16 1989-11-21 The Research Foundation Of State University Of New York Novel polynucleotide analogs, methods for inhibiting nucleic acid polymerases and methods for inducing synthesis of interferon
US4971736A (en) 1987-12-28 1990-11-20 Minnesota Mining And Manufacturing Company Method of preparing composite chromatographic article
US4810381A (en) 1987-12-28 1989-03-07 Minnesota Mining And Manufacturing Company Composite chromatographic article
US4906378A (en) 1987-12-28 1990-03-06 Minnesota Mining And Manufacturing Company Composite chromatographic article
US4923978A (en) 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
DE3921498A1 (de) 1988-09-28 1990-03-29 Bayer Ag Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung
US4957943A (en) 1988-10-14 1990-09-18 Minnesota Mining And Manufacturing Company Particle-filled microporous materials
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JPH02268682A (ja) 1989-04-07 1990-11-02 Hitachi Ltd Dna試料調製方法
JPH0824583B2 (ja) 1989-05-09 1996-03-13 株式会社ニッショー Dnaの抽出方法
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
US5207915A (en) 1990-02-23 1993-05-04 Minnesota Mining And Manufacturing Company Separation method using controlled pore composite polytetrafluoroethylene article
US5071610A (en) 1990-02-23 1991-12-10 Minnesota Mining And Manufacturing Company Method of making a controlled pore composite polytetrafluoroethylene
US5147539A (en) 1990-02-23 1992-09-15 Minnesota Mining And Manufacturing Company Controlled pore composite polytetrafluoroethylene article
US5187083A (en) 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
CA2059398C (en) 1991-02-07 1999-05-25 Craig G. Markell Solid phase extraction medium
AU1978692A (en) 1991-04-12 1992-11-17 Minnesota Mining And Manufacturing Company Purification of nucleic acids using metal oxide supports
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5238621A (en) 1991-06-28 1993-08-24 Minnesota Mining And Manufacturing Company Method of controlling porosity in a composite article
FR2679255B1 (fr) 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
US5438128A (en) 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
CA2096193A1 (en) 1992-06-01 1993-12-02 Daniel L. Woodard Chemically synthesized silanes that bind nucleic acids
JPH07508518A (ja) 1992-06-23 1995-09-21 ミネソタ マイニング アンド マニュファクチャリング カンパニー アズラクトン官能支持体による脱タンパク質
CA2102264C (en) 1992-11-13 2000-08-01 Daniel Lee Woodard Boron silicates, aluminum silicates, phosphosilicates and purification of dna
AT398973B (de) 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US5438129A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
KR100230909B1 (ko) 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US5625053A (en) 1994-08-26 1997-04-29 Board Of Regents For Northern Illinois Univ. Method of isolating purified plasmid DNA using a nonionic detergent, solution
US5472600A (en) 1995-02-01 1995-12-05 Minnesota Mining And Manufacturing Company Gradient density filter
JPH09302A (ja) 1995-06-16 1997-01-07 Rieko Moto 履 物
DE19530132C2 (de) 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
JPH09302034A (ja) 1996-05-14 1997-11-25 Makoto Komiyama 核酸誘導体および尿素の吸着除去可能な高分子材料
JPH10155481A (ja) * 1996-06-18 1998-06-16 Rikagaku Kenkyusho Dnaの回収方法
US6093559A (en) * 1996-10-24 2000-07-25 Corning Incorporated Producing low binding hydrophobic surfaces by treating with a low HLB number non-ionic surfactant
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
EP0897978A3 (de) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirkoniumoxyd und verwandten Verbindungen zur Reinigung von Nukleinsäuren
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
FI974124A0 (fi) 1997-11-04 1997-11-04 Satu Aokerman Foerfarande foer separering av proteinfria aemnen fraon proteinhaltiga aemnen foer daerpao foeljande behandling
US6475722B1 (en) 1997-12-03 2002-11-05 Curagen Corporation Surface treatments for DNA processing devices
DE69839133T2 (de) * 1997-12-06 2009-02-05 Invitrogen Corp., Carlsbad Isolierung von Nukleinsäuren
DE19907023A1 (de) * 1999-02-19 2000-08-24 Bayer Ag Verfahren zur Isolierung von Nucleinsäuren
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant

Also Published As

Publication number Publication date
DE60012549T2 (de) 2005-09-01
US20030018177A1 (en) 2003-01-23
US6790642B2 (en) 2004-09-14
AU2745000A (en) 2001-04-24
JP2003510254A (ja) 2003-03-18
US6383783B1 (en) 2002-05-07
EP1216255A1 (de) 2002-06-26
EP1216255B1 (de) 2004-07-28
WO2001021632A1 (en) 2001-03-29

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Legal Events

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