WO2001042493A3 - Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna - Google Patents

Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna Download PDF

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Publication number
WO2001042493A3
WO2001042493A3 PCT/DE2000/004381 DE0004381W WO0142493A3 WO 2001042493 A3 WO2001042493 A3 WO 2001042493A3 DE 0004381 W DE0004381 W DE 0004381W WO 0142493 A3 WO0142493 A3 WO 0142493A3
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WO
WIPO (PCT)
Prior art keywords
genomic dna
methylation
degree
parallel detection
steps
Prior art date
Application number
PCT/DE2000/004381
Other languages
English (en)
French (fr)
Other versions
WO2001042493A2 (de
Inventor
Alexander Olek
Christian Piepenbrock
Original Assignee
Epigenomics Ag
Alexander Olek
Christian Piepenbrock
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Alexander Olek, Christian Piepenbrock filed Critical Epigenomics Ag
Priority to CA002395047A priority Critical patent/CA2395047A1/en
Priority to US10/149,109 priority patent/US20040248090A1/en
Priority to DE10083729T priority patent/DE10083729D2/de
Priority to AU26632/01A priority patent/AU778411B2/en
Priority to EP00989842A priority patent/EP1238112A2/de
Publication of WO2001042493A2 publication Critical patent/WO2001042493A2/de
Publication of WO2001042493A3 publication Critical patent/WO2001042493A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Beschrieben ist ein Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA bei dem man folgende Schritte ausführt: (a) in einer genomischen DNA Probe wandelt man durch chemische Behandlung an der 5'-Position unmethylierte Cytosinbasen in Uracil, Thymidin oder eine andere vom Hybridisierungsverhalten her dem Cytosin unähnliche Base um; (b) aus dieser chemisch behandelten genomischen DNA amplifiziert man mehr als zehn unterschiedliche Fragmente, die jeweils weniger als 2000 Basenpaare lang sind, gleichzeitig durch Verwendung von synthetischen Oligonukleotiden als Primer, wobei diese Primer jeweils Sequenzen aus an der Genregulation beteiligten und/oder transkribierten und/oder translatierten genomischen Sequenzen enthalten, wie sie nach einer Behandlung gemäß Schritt (a) vorliegen würden; (c) man bestimmt den Sequenzkontext aller oder eines Teils der in den amplifizierten Fragmenten enthaltenen CpG Dinukleotide oder CpNpG Trinukleotide.
PCT/DE2000/004381 1999-12-06 2000-12-06 Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna WO2001042493A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002395047A CA2395047A1 (en) 1999-12-06 2000-12-06 Method for the parallel detection of the degree of methylation of genomic dna
US10/149,109 US20040248090A1 (en) 1999-12-06 2000-12-06 Method for the parallel detection of the degree of methylation of genomic dna
DE10083729T DE10083729D2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen Detektion des Methylierungszustandes von genomischer DNA
AU26632/01A AU778411B2 (en) 1999-12-06 2000-12-06 Method for the parallel detection of the degree of methylation of genomic DNA
EP00989842A EP1238112A2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19959691A DE19959691A1 (de) 1999-12-06 1999-12-06 Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
DE19959691.3 1999-12-06

Publications (2)

Publication Number Publication Date
WO2001042493A2 WO2001042493A2 (de) 2001-06-14
WO2001042493A3 true WO2001042493A3 (de) 2002-02-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/004381 WO2001042493A2 (de) 1999-12-06 2000-12-06 Verfahren zur parallelen detektion des methylierungszustandes von genomischer dna

Country Status (6)

Country Link
US (1) US20040248090A1 (de)
EP (1) EP1238112A2 (de)
AU (1) AU778411B2 (de)
CA (1) CA2395047A1 (de)
DE (2) DE19959691A1 (de)
WO (1) WO2001042493A2 (de)

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US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
AUPR142500A0 (en) * 2000-11-13 2000-12-07 Human Genetic Signatures Pty Ltd A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences
DE10151055B4 (de) * 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1567669B1 (de) * 2002-12-02 2010-03-24 Illumina Cambridge Limited Bestimmung der methylierung von nukleinsäuresequenzen
DE60333680D1 (de) * 2003-03-13 2010-09-16 Beijing Inst Cancer Res VERFAHREN ZUR IN-VITRO-ERKENNUNG VON ANORMALER Magen-DYSPLASIE
DE602004028478D1 (de) 2003-06-17 2010-09-16 Human Genetic Signatures Pty Verfahren zur genomamplifikation
DE602004018801D1 (de) 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nukleinsäurenachweistest
US7365058B2 (en) 2004-04-13 2008-04-29 The Rockefeller University MicroRNA and methods for inhibiting same
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DE602005022740D1 (de) 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
CN101111606B (zh) 2004-12-03 2012-05-16 人类遗传标记控股有限公司 通过化学修饰胞嘧啶而简化微生物核酸的方法
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
JP2008541705A (ja) 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
ATE531820T1 (de) 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
US8119788B2 (en) * 2005-09-27 2012-02-21 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Compositions and methods for the detection of Candida species
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
EP2215250B1 (de) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzyme zur amplifikation und zum kopieren bisulphit-modifizierter nukleinsäuren
US8361746B2 (en) * 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
EP2322656A1 (de) * 2009-11-17 2011-05-18 Centre National de la Recherche Scientifique (C.N.R.S) Verfahren zur Diagnose von Hautkrankheiten
CA2848144C (en) 2011-09-07 2018-03-06 Human Genetic Signatures Pty Ltd Molecular detection assay
CN105378107A (zh) * 2013-03-14 2016-03-02 雅培分子公司 多重甲基化-特异性扩增系统和方法

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1998056952A1 (en) * 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences
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WO1999055905A1 (en) * 1998-04-23 1999-11-04 Commonwealth Scientific And Industrial Research Organisation ASSAY FOR METHYLATION IN THE GST-Pi GENE

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Patent Citations (4)

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WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1998056952A1 (en) * 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences
WO1999028498A2 (de) * 1997-11-27 1999-06-10 Epigenomics Gmbh Verfahren zur herstellung komplexer dna-methylierungs-fingerabdrücke
WO1999055905A1 (en) * 1998-04-23 1999-11-04 Commonwealth Scientific And Industrial Research Organisation ASSAY FOR METHYLATION IN THE GST-Pi GENE

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Also Published As

Publication number Publication date
DE10083729D2 (de) 2003-05-15
AU778411B2 (en) 2004-12-02
DE19959691A1 (de) 2001-08-16
AU2663201A (en) 2001-06-18
US20040248090A1 (en) 2004-12-09
WO2001042493A2 (de) 2001-06-14
CA2395047A1 (en) 2001-06-14
EP1238112A2 (de) 2002-09-11

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