WO2001023618A3 - Compositions and methods for reducing oligonucleotide hybridization and priming specificity - Google Patents

Compositions and methods for reducing oligonucleotide hybridization and priming specificity Download PDF

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Publication number
WO2001023618A3
WO2001023618A3 PCT/US2000/026775 US0026775W WO0123618A3 WO 2001023618 A3 WO2001023618 A3 WO 2001023618A3 US 0026775 W US0026775 W US 0026775W WO 0123618 A3 WO0123618 A3 WO 0123618A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
oligonucleotide hybridization
priming specificity
apcs
Prior art date
Application number
PCT/US2000/026775
Other languages
French (fr)
Other versions
WO2001023618A2 (en
Inventor
Ness Jeffrey Van
Lori K Garrison
Original Assignee
Qiagen Genomics Inc
Ness Jeffrey Van
Lori K Garrison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Genomics Inc, Ness Jeffrey Van, Lori K Garrison filed Critical Qiagen Genomics Inc
Priority to AU77332/00A priority Critical patent/AU7733200A/en
Publication of WO2001023618A2 publication Critical patent/WO2001023618A2/en
Publication of WO2001023618A3 publication Critical patent/WO2001023618A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Abstract

Annealing promoting compounds (APCs) are used to reduce the hybridization specificity of oligonucleotide duplexes containing one or more nucleotide mismatches as compared to perfectly base-paired oligonucleotide duplexes. These APCs are used in methods for the detection of target oligonucleotides that vary in nucleic acid sequence by one or more nucleic acid as compared to a control sequence.
PCT/US2000/026775 1999-09-30 2000-09-28 Compositions and methods for reducing oligonucleotide hybridization and priming specificity WO2001023618A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU77332/00A AU7733200A (en) 1999-09-30 2000-09-28 Compositions and methods for reducing oligonucleotide hybridization and priming specificity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15715999P 1999-09-30 1999-09-30
US60/157,159 1999-09-30

Publications (2)

Publication Number Publication Date
WO2001023618A2 WO2001023618A2 (en) 2001-04-05
WO2001023618A3 true WO2001023618A3 (en) 2002-04-18

Family

ID=22562580

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/026775 WO2001023618A2 (en) 1999-09-30 2000-09-28 Compositions and methods for reducing oligonucleotide hybridization and priming specificity

Country Status (2)

Country Link
AU (1) AU7733200A (en)
WO (1) WO2001023618A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60228750D1 (en) * 2001-12-08 2008-10-16 Seegene Inc ANNEALING CONTROL PRIMER AND ITS USES
AU2002222737A1 (en) * 2001-12-08 2003-06-23 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
AU2002307654A1 (en) * 2002-05-01 2003-11-17 Seegene, Inc. Methods and compositions for improving specificity of pcr amplication
KR100812259B1 (en) * 2006-06-12 2008-03-10 주식회사 씨젠 Method for Amplifying Unknown Sequence Adjacent to Known Sequence
US9476095B2 (en) 2011-04-15 2016-10-25 The Johns Hopkins University Safe sequencing system
EP3447495B2 (en) 2012-10-29 2024-03-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
CN111868260A (en) 2017-08-07 2020-10-30 约翰斯霍普金斯大学 Methods and materials for assessing and treating cancer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996034115A1 (en) * 1995-04-27 1996-10-31 Cis Bio International Buffer and method for evaluating optimal nucleic acid target sequence amplification conditions and applications thereof
WO1998013527A2 (en) * 1996-09-24 1998-04-02 Rapigene, Inc. Compositions and methods for enhancing hybridization specificity
WO1999041410A1 (en) * 1998-02-10 1999-08-19 Yeda Research And Development Co. Ltd. Methods for dna amplification and sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996034115A1 (en) * 1995-04-27 1996-10-31 Cis Bio International Buffer and method for evaluating optimal nucleic acid target sequence amplification conditions and applications thereof
WO1998013527A2 (en) * 1996-09-24 1998-04-02 Rapigene, Inc. Compositions and methods for enhancing hybridization specificity
WO1999041410A1 (en) * 1998-02-10 1999-08-19 Yeda Research And Development Co. Ltd. Methods for dna amplification and sequencing

Also Published As

Publication number Publication date
AU7733200A (en) 2001-04-30
WO2001023618A2 (en) 2001-04-05

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