WO2003057909A3 - Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla) - Google Patents

Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla) Download PDF

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Publication number
WO2003057909A3
WO2003057909A3 PCT/DE2003/000073 DE0300073W WO03057909A3 WO 2003057909 A3 WO2003057909 A3 WO 2003057909A3 DE 0300073 W DE0300073 W DE 0300073W WO 03057909 A3 WO03057909 A3 WO 03057909A3
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WO
WIPO (PCT)
Prior art keywords
dna
nucleotides
mla
ligation
exponential
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PCT/DE2003/000073
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English (en)
French (fr)
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WO2003057909A2 (de
Inventor
Kurt Berlin
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Epigenomics Ag
Kurt Berlin
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Publication date
Application filed by Epigenomics Ag, Kurt Berlin filed Critical Epigenomics Ag
Priority to EP03704206A priority Critical patent/EP1463841A2/de
Priority to AU2003206620A priority patent/AU2003206620B2/en
Priority to US10/501,040 priority patent/US20050064428A1/en
Priority to JP2003558202A priority patent/JP2005514035A/ja
Publication of WO2003057909A2 publication Critical patent/WO2003057909A2/de
Publication of WO2003057909A3 publication Critical patent/WO2003057909A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

Beschrieben wird ein Verfahren zum Nachweis von Cytosin-Methylierung in DNA-Proben, das aus den folgenden Schritten besteht: Zuerst wird eine genomische DNA-Probe, welche zu untersuchende DNA und Hintergrund-DNA umfasst, chemisch derart behandelt, dass alle nicht methylierten Cytosinbasen in Uracil umgewandelt werden, während die 5-Methylcytosinbasen unverändert bleiben. Dann wird die chemisch behandelte DNA-Probe unter Verwendung von mindestens 2 Primeroligonukleotiden sowie einer Polymerase amplifiziert, wobei die zu untersuchende DNA gegenüber der Hintergrund-DNA als Templat bevorzugt wird und im letzten Schritt werden die Amplifikate analysiert und aus dem Vorliegen eines Amplifikates und/oder aus der Analyse weiterer Positionen auf den Methylierungsstatus in der zu untersuchenden DNA geschlossen.
PCT/DE2003/000073 2002-01-08 2003-01-08 Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla) WO2003057909A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03704206A EP1463841A2 (de) 2002-01-08 2003-01-08 Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla)
AU2003206620A AU2003206620B2 (en) 2002-01-08 2003-01-08 Method for detecting cytosine-methylation patterns by exponential ligation of hybridised probe oligo-nucleotides (MLA)
US10/501,040 US20050064428A1 (en) 2002-01-08 2003-01-08 Method for detecting cytosine-methylation patterns by exponential ligation of hybridised probe oligo-nucleotides (mla)
JP2003558202A JP2005514035A (ja) 2002-01-08 2003-01-08 ハイブリダイゼーションしたプローブオリゴヌクレオチド（ｍｌａ）の指数的ライゲーションによるシトシン−メチル化パターンの検出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10201138A DE10201138B4 (de) 2002-01-08 2002-01-08 Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
DE10201138.9 2002-01-08

Publications (2)

Publication Number Publication Date
WO2003057909A2 WO2003057909A2 (de) 2003-07-17
WO2003057909A3 true WO2003057909A3 (de) 2003-10-16

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PCT/DE2003/000073 WO2003057909A2 (de) 2002-01-08 2003-01-08 Verfahren zum nachweis von cytosin-methylierungsmustern durch exponentielle ligation hybridisierter sondenoligonukleotide (mla)

Country Status (6)

Country Link
US (1) US20050064428A1 (de)
EP (1) EP1463841A2 (de)
JP (1) JP2005514035A (de)
AU (1) AU2003206620B2 (de)
DE (1) DE10201138B4 (de)
WO (1) WO2003057909A2 (de)

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US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis

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US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
CN1774511B (zh) * 2002-11-27 2013-08-21 斯昆诺有限公司 用于序列变异检测和发现的基于断裂的方法和系统
AU2004235331B2 (en) * 2003-04-25 2008-12-18 Sequenom, Inc. Fragmentation-based methods and systems for De Novo sequencing
US7608394B2 (en) * 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
US20080070240A2 (en) * 2004-04-08 2008-03-20 Toyo Boseki Kabushiki Kaisha Composition for deaminating dna and method of detecting methylated dna
US7364855B2 (en) 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
AU2005284980A1 (en) * 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20070196849A1 (en) * 2006-02-22 2007-08-23 Applera Corporation Double-ligation Method for Haplotype and Large-scale Polymorphism Detection
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8206927B2 (en) 2007-01-23 2012-06-26 Sequenom, Inc. Method for accurate assessment of DNA quality after bisulfite treatment
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
US8709726B2 (en) * 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20110143959A1 (en) * 2008-08-13 2011-06-16 Rosetta Genomics Ltd. Compositions and methods for determining the prognosis of bladder urothelial cancer
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9624530B2 (en) 2009-08-03 2017-04-18 Epigenomics Ag Methods for preservation of genomic DNA sequence complexity
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
EP2516680B1 (de) 2009-12-22 2016-04-06 Sequenom, Inc. Verfahren und kits zur identifizierung von aneuploidie
CN103717750B (zh) 2011-04-29 2017-03-08 塞昆纳姆股份有限公司 少数核酸物质的定量
EP4155401A1 (de) 2012-03-02 2023-03-29 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
JP6222639B2 (ja) * 2013-03-07 2017-11-01 積水メディカル株式会社 メチル化ｄｎａの検出方法
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
WO2021192395A1 (ja) 2020-03-25 2021-09-30 富士フイルム株式会社 塩基のメチル化度の算出方法及びプログラム

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses

Also Published As

Publication number Publication date
US20050064428A1 (en) 2005-03-24
AU2003206620A1 (en) 2003-07-24
AU2003206620B2 (en) 2007-08-09
EP1463841A2 (de) 2004-10-06
WO2003057909A2 (de) 2003-07-17
DE10201138A1 (de) 2003-07-17
JP2005514035A (ja) 2005-05-19
DE10201138B4 (de) 2005-03-10

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