ATE417938T1 - Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren - Google Patents

Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Info

Publication number
ATE417938T1
ATE417938T1 AT05722543T AT05722543T ATE417938T1 AT E417938 T1 ATE417938 T1 AT E417938T1 AT 05722543 T AT05722543 T AT 05722543T AT 05722543 T AT05722543 T AT 05722543T AT E417938 T1 ATE417938 T1 AT E417938T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
amplification process
novel nucleotide
nucleotide mixture
Prior art date
Application number
AT05722543T
Other languages
English (en)
Inventor
Brian Washburn
Douglas Lovern
Original Assignee
Bio Merieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux Inc filed Critical Bio Merieux Inc
Application granted granted Critical
Publication of ATE417938T1 publication Critical patent/ATE417938T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT05722543T 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren ATE417938T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53881404P 2004-01-23 2004-01-23
US53881504P 2004-01-23 2004-01-23
US53881604P 2004-01-23 2004-01-23

Publications (1)

Publication Number Publication Date
ATE417938T1 true ATE417938T1 (de) 2009-01-15

Family

ID=34812082

Family Applications (3)

Application Number Title Priority Date Filing Date
AT08075840T ATE491810T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung
AT05712046T ATE481507T1 (de) 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
AT05722543T ATE417938T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Family Applications Before (2)

Application Number Title Priority Date Filing Date
AT08075840T ATE491810T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung
AT05712046T ATE481507T1 (de) 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv

Country Status (6)

Country Link
US (3) US7364854B2 (de)
EP (3) EP2042612B1 (de)
JP (2) JP4866248B2 (de)
AT (3) ATE491810T1 (de)
DE (3) DE602005023586D1 (de)
WO (2) WO2005070027A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2147118A4 (de) * 2007-04-27 2010-09-01 Eragen Biosciences Inc Materialien und verfahren zum nachweis von hepatitis-c-virus
US8435741B2 (en) * 2007-07-26 2013-05-07 Fujifilm Corporation Isothermal nucleic acid amplification method using surfactant
US20090162856A1 (en) * 2007-11-06 2009-06-25 Hayato Miyoshi Rna detection method
US20100261154A1 (en) * 2008-12-31 2010-10-14 Abbott Laboratories Primers and probes for detecting hepatitis c virus

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09107970A (ja) * 1995-10-17 1997-04-28 Soyaku Gijutsu Kenkyusho:Kk Ｃ型肝炎ウイルス由来核酸、該核酸を用いたウイルスの検出方法
EP0052296B1 (de) * 1980-11-12 1984-06-27 Mitsubishi Kasei Corporation Als Heilmittel für Immunokrankheiten anwendbare Glutaminderivate, Verfahren zu deren Herstellung und Zusammensetzungen, die die obengenannten Derivate enthalten
US5087565A (en) * 1984-07-02 1992-02-11 Du Pont Merck Pharmaceutical Company Synthetic process for preparation of 32 P-labeled nucleotides
WO1986006726A1 (en) 1985-05-15 1986-11-20 Integrated Genetics, Inc. Cytidine analogs
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5032511A (en) * 1987-03-31 1991-07-16 Mitsubishi Kasei Corporation DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5714596A (en) * 1987-11-18 1998-02-03 Chiron Corporation NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus
US5712088A (en) * 1987-11-18 1998-01-27 Chiron Corporation Methods for detecting Hepatitis C virus using polynucleotides specific for same
US5698390A (en) * 1987-11-18 1997-12-16 Chiron Corporation Hepatitis C immunoassays
US5030557A (en) 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
DE69025958T2 (de) 1989-09-18 1996-11-14 Canon Kk Tintenstrahlaufzeichnungskopf und Tintenstrahlgerät mit diesem Kopf
WO1991012342A1 (en) * 1990-02-16 1991-08-22 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
WO2002081494A1 (en) * 2001-03-26 2002-10-17 Sirna Therapeutics, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication
US5648232A (en) * 1993-01-21 1997-07-15 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Microbiological best method and reagents
CA2184270C (en) * 1994-03-10 1999-08-17 Daniel Louis Kacian Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents
US5874565A (en) * 1995-08-29 1999-02-23 Washington University Nucleic acids comprising a highly conserved novel 3 terminal sequence element of the hepatitis C virus
US6127116A (en) * 1995-08-29 2000-10-03 Washington University Functional DNA clone for hepatitis C virus (HCV) and uses thereof
DE19612779A1 (de) * 1996-03-29 1997-10-02 Boehringer Mannheim Gmbh Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR
AU4065397A (en) * 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
DE19647055A1 (de) * 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilisierte wässrige Nukleosidtriphosphat-Lösungen
DK1705253T3 (da) * 1997-04-03 2011-02-21 Life Technologies Corp Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)
ZA989950B (en) 1997-11-17 1999-05-04 Akzo Nobel Nv Transcription based amplification of double stranded DNA targets
JP2002512791A (ja) * 1998-04-27 2002-05-08 リボザイム・ファーマシューティカルズ・インコーポレーテッド Ｃ型肝炎ウイルス感染に関連する疾患または状態の酵素的核酸治療
WO2000000638A2 (en) * 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US6638714B1 (en) * 1999-02-03 2003-10-28 Ortho-Clinical Diagnostics, Inc. Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof
EP1185664B1 (de) * 1999-06-04 2007-05-16 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY of the DEPARTMENT OF HEALTH AND HUMAN SERVICES Klonierte genome von infektiösen hepatitis c viren genotyp 2a und deren verwendungen
US6271004B1 (en) 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
WO2001036442A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
WO2001064959A1 (en) * 2000-03-02 2001-09-07 Akzo Nobel N.V. Detection of hepatitis b virus rna
WO2003016572A1 (en) * 2001-08-17 2003-02-27 Eli Lilly And Company Oligonucleotide therapeutics for treating hepatitis c virus infections
US6783940B2 (en) 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
US6750009B2 (en) * 2002-01-29 2004-06-15 Apath, Llc Multiple viral replicon culture systems
AU2003241614A1 (en) * 2002-05-24 2003-12-12 Smithkline Beecham Corporation Method for quantitating negative strand rna synthesis

Also Published As

Publication number Publication date
EP1721015B1 (de) 2010-09-15
EP1706512B1 (de) 2008-12-17
EP1721015A2 (de) 2006-11-15
US20060003343A1 (en) 2006-01-05
ATE481507T1 (de) 2010-10-15
WO2005070027A8 (en) 2006-07-27
WO2005071117A2 (en) 2005-08-04
EP2042612B1 (de) 2010-12-15
DE602005025424D1 (de) 2011-01-27
WO2005070027A3 (en) 2006-04-06
DE602005011754D1 (de) 2009-01-29
JP2007518425A (ja) 2007-07-12
US7364854B2 (en) 2008-04-29
US7670779B2 (en) 2010-03-02
WO2005071117A3 (en) 2006-04-20
US20080248480A1 (en) 2008-10-09
EP1706512A2 (de) 2006-10-04
JP2007518424A (ja) 2007-07-12
EP2042612A2 (de) 2009-04-01
WO2005070027A2 (en) 2005-08-04
ATE491810T1 (de) 2011-01-15
EP2042612A3 (de) 2009-06-17
DE602005023586D1 (de) 2010-10-28
JP4866248B2 (ja) 2012-02-01
US20060019266A1 (en) 2006-01-26

Similar Documents

Publication Publication Date Title
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
ATE549419T1 (de) Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
EP1350841A3 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
MY143596A (en) In vitro recombination method
WO2008033442A3 (en) Methods and compositions for performing low background multiplex nucleic acid amplification reactions
DE602006017405D1 (de) Verfahren zur in vitro-rekombination
ATE502122T1 (de) Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
DE60329853D1 (de) Verbesserte zusammensetzungen und verfahren für die cdna-synthese
DE112006002611A5 (de) Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna
DE602005017252D1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
ATE348881T1 (de) Thermostabiles taq polymerase fragment
DE602005009693D1 (de) 2"-nitrobenzyl-modifizierte ribonukleotide
SE0301951D0 (sv) New method
ATE555196T1 (de) Wärmebeständige duplexspezifische nuklease
ATE417938T1 (de) Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren
DK1504098T4 (da) Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
DE602008004702D1 (de) Verfahren zur Amplifikation von Nukleinsäure
WO2003004703A1 (en) Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same
ATE533847T1 (de) Verfahren für den nachweis und die quantifizierung einer endogenen dna-sequenz aus weizen
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
ATE504648T1 (de) Rna-interferenz für das gen znfn3a1 als verfahren zur hemmung des wachstums von krebszellen
WO2010039189A3 (en) Methods for sequencing degraded or modified nucleic acids
ATE399868T1 (de) Optimierte nukleotidsequenzen die für sgp130 kodieren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties