DE602007013223D1 - Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation - Google Patents
Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikationInfo
- Publication number
- DE602007013223D1 DE602007013223D1 DE602007013223T DE602007013223T DE602007013223D1 DE 602007013223 D1 DE602007013223 D1 DE 602007013223D1 DE 602007013223 T DE602007013223 T DE 602007013223T DE 602007013223 T DE602007013223 T DE 602007013223T DE 602007013223 D1 DE602007013223 D1 DE 602007013223D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- acid amplification
- oligonucleotide primers
- modified oligonucleotide
- chemically modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003155 DNA primer Substances 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 125000003636 chemical group Chemical group 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 125000004185 ester group Chemical group 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81066506P | 2006-06-01 | 2006-06-01 | |
| PCT/US2007/011950 WO2007139723A1 (en) | 2006-06-01 | 2007-05-17 | Chemicallymodified oligonucleotide primers for nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602007013223D1 true DE602007013223D1 (de) | 2011-04-28 |
Family
ID=38778971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602007013223T Active DE602007013223D1 (de) | 2006-06-01 | 2007-05-17 | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8361753B2 (de) |
| EP (1) | EP2032714B1 (de) |
| JP (1) | JP5558811B2 (de) |
| CN (1) | CN101517091B (de) |
| AT (1) | ATE502122T1 (de) |
| AU (1) | AU2007268075C1 (de) |
| CA (1) | CA2653841C (de) |
| DE (1) | DE602007013223D1 (de) |
| ES (1) | ES2360738T3 (de) |
| HK (1) | HK1129045A1 (de) |
| WO (1) | WO2007139723A1 (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4082551A1 (de) | 2006-08-08 | 2022-11-02 | Rheinische Friedrich-Wilhelms-Universität Bonn | Struktur und verwendung von 5'-phosphat-oligonukleotiden |
| EP2113574A1 (de) * | 2008-04-28 | 2009-11-04 | Biotype AG | Stoffe und Verfahren für Profilierungstest auf DNA-Basis |
| JP5539325B2 (ja) | 2008-04-30 | 2014-07-02 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| JP5689413B2 (ja) | 2008-05-21 | 2015-03-25 | ライニッシュ フリードリッヒ−ウィルヘルムズ−ユニバーシタット ボン | 平滑末端を有する５’三リン酸オリゴヌクレオチドおよびその使用 |
| JP5712125B2 (ja) * | 2008-05-27 | 2015-05-07 | トリリンク バイオテクノロジーズ | 高温で開始される核酸増幅のための、化学修飾されたヌクレオシド５’−三リン酸 |
| CN101638685B (zh) * | 2008-07-29 | 2013-01-09 | 杭州优思达生物技术有限公司 | 交叉引物扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
| JP2012514461A (ja) * | 2009-01-06 | 2012-06-28 | ヨウ，キミン | 標的核酸の交差プライミング増幅 |
| CA2777446A1 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| WO2012094343A1 (en) * | 2011-01-05 | 2012-07-12 | Trilink Biotechnologies | Chemically substituted thermosensitive probes and cofactors for hot start ligation |
| AU2012236896A1 (en) | 2011-03-25 | 2013-05-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CA2854907C (en) * | 2011-11-18 | 2020-03-10 | Sarepta Therapeutics, Inc. | Functionally-modified oligonucleotides and subunits thereof |
| US9353393B2 (en) | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US9150896B2 (en) * | 2012-09-06 | 2015-10-06 | Illumina, Inc. | Nucleotides and primers with removable blocking groups |
| EP2712870A1 (de) | 2012-09-27 | 2014-04-02 | Rheinische Friedrich-Wilhelms-Universität Bonn | Neuartige RIG-I-Liganden und Herstellungsverfahren dafür |
| CN105612254B (zh) | 2013-10-07 | 2020-06-23 | 三井化学株式会社 | 用于扩增细菌dna的pcr中使用的引物组及其试剂盒和方法 |
| US9382582B1 (en) * | 2015-08-25 | 2016-07-05 | Tracy Hayden | Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction |
| EP3906789B1 (de) | 2015-09-21 | 2023-11-22 | TriLink BioTechnologies, LLC | Initiierende verkappte oligonukleotidprimer zur synthese von 5'-verkappten rnas |
| US11371087B2 (en) | 2016-06-10 | 2022-06-28 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| EP3486321A4 (de) * | 2016-06-23 | 2020-01-15 | Riken | T-zell-rezeptor und b-zell-rezeptor-repertoire-analysesystem unter verwendung einer template-switching-polymerasekettenraktion mit einstufiger reverser transkription |
| WO2019074091A1 (ja) | 2017-10-12 | 2019-04-18 | 三井化学株式会社 | ｍｅｃＡ遺伝子増幅用プライマーペア、ｍｅｃＡ遺伝子検出キット及びｍｅｃＡ遺伝子検出方法 |
| EP3837380A4 (de) | 2018-08-17 | 2022-05-11 | Cellecta, Inc. | Multiplex-präparation von barcodierten genspezifischen dns-fragmenten |
| TW202031888A (zh) | 2018-08-24 | 2020-09-01 | 日商組庫創世紀股份有限公司 | T細胞受體及b細胞受體之功能性次單元成對基因之解析方法 |
| CN109680342B (zh) * | 2018-12-18 | 2021-09-28 | 上海药明康德新药开发有限公司 | DNA编码化合物库中On-DNA芳硝基化合物还原成On-DNA芳胺化合物的方法 |
| WO2020214589A1 (en) * | 2019-04-17 | 2020-10-22 | Sena Research, Incorporated | Modified nucleotides and methods for dna and rna polymerization and sequencing |
| US11739359B2 (en) | 2020-05-01 | 2023-08-29 | Microsoft Technology Licensing, Llc | Universal template strands for enzymatic polynucleotide synthesis |
| US11702683B2 (en) | 2020-05-28 | 2023-07-18 | Microsoft Technology Licensing, Llc | De novo polynucleotide synthesis with substrate-bound polymerase |
| IL314442A (en) | 2022-01-27 | 2024-09-01 | Trilink Biotechnologies Llc | Trinucleotide cap analogs and methods of using them |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5852188A (en) * | 1990-01-11 | 1998-12-22 | Isis Pharmaceuticals, Inc. | Oligonucleotides having chiral phosphorus linkages |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US6057134A (en) * | 1996-10-07 | 2000-05-02 | Ambion, Inc. | Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides |
| US6399304B1 (en) * | 1996-12-20 | 2002-06-04 | Roche Diagnostics Gmbh | Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules |
| US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| DK0866071T3 (da) * | 1997-03-20 | 2005-01-17 | Hoffmann La Roche | Modificerede primere |
| CA2370478A1 (en) * | 1999-03-24 | 2000-09-28 | Serge L. Beaucage | N-acylphosphoramidites and their use in oligonucleotide synthesis |
| US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| WO2001075139A1 (en) * | 2000-04-03 | 2001-10-11 | Biolink Partners, Inc. | Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization |
| WO2002018616A1 (en) | 2000-09-01 | 2002-03-07 | Hitachi Chemical Co., Ltd. | Adjusting the efficiency of nucleic acid template amplification by attenuated pcr with template-mimic oligonucleotides |
| US6743905B2 (en) * | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| US7700281B2 (en) | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| GB2416352B (en) | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| WO2006074217A2 (en) * | 2005-01-04 | 2006-07-13 | Stratagene California | Hot start polymerase reaction using a thermolabile blocker |
| WO2007011901A2 (en) | 2005-07-15 | 2007-01-25 | Applera Corporation | Hot start reverse transcription by primer design |
-
2007
- 2007-05-17 AT AT07777161T patent/ATE502122T1/de not_active IP Right Cessation
- 2007-05-17 ES ES07777161T patent/ES2360738T3/es active Active
- 2007-05-17 EP EP07777161A patent/EP2032714B1/de active Active
- 2007-05-17 CN CN200780027958.8A patent/CN101517091B/zh active Active
- 2007-05-17 US US11/750,237 patent/US8361753B2/en active Active
- 2007-05-17 WO PCT/US2007/011950 patent/WO2007139723A1/en active Application Filing
- 2007-05-17 DE DE602007013223T patent/DE602007013223D1/de active Active
- 2007-05-17 JP JP2009513171A patent/JP5558811B2/ja active Active
- 2007-05-17 CA CA2653841A patent/CA2653841C/en active Active
- 2007-05-17 AU AU2007268075A patent/AU2007268075C1/en active Active
-
2009
- 2009-09-04 HK HK09108152.8A patent/HK1129045A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE502122T1 (de) | 2011-04-15 |
| AU2007268075A1 (en) | 2007-12-06 |
| CA2653841A1 (en) | 2007-12-06 |
| US8361753B2 (en) | 2013-01-29 |
| CN101517091A (zh) | 2009-08-26 |
| ES2360738T3 (es) | 2011-06-08 |
| EP2032714A4 (de) | 2009-12-23 |
| EP2032714A1 (de) | 2009-03-11 |
| WO2007139723A1 (en) | 2007-12-06 |
| AU2007268075B2 (en) | 2012-09-13 |
| JP5558811B2 (ja) | 2014-07-23 |
| US20070281308A1 (en) | 2007-12-06 |
| EP2032714B1 (de) | 2011-03-16 |
| WO2007139723A8 (en) | 2009-12-17 |
| CN101517091B (zh) | 2013-02-13 |
| HK1129045A1 (en) | 2009-11-13 |
| JP2009538621A (ja) | 2009-11-12 |
| AU2007268075C1 (en) | 2013-03-07 |
| CA2653841C (en) | 2016-07-19 |
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