DE602007013223D1 - Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation - Google Patents

Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation

Info

Publication number
DE602007013223D1
DE602007013223D1 DE602007013223T DE602007013223T DE602007013223D1 DE 602007013223 D1 DE602007013223 D1 DE 602007013223D1 DE 602007013223 T DE602007013223 T DE 602007013223T DE 602007013223 T DE602007013223 T DE 602007013223T DE 602007013223 D1 DE602007013223 D1 DE 602007013223D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid amplification
oligonucleotide primers
modified oligonucleotide
chemically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602007013223T
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English (en)
Inventor
Gerald Zon
Alexandre Lebedev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Trilink Biotechnologies LLC
Original Assignee
Trilink Biotechnologies LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Trilink Biotechnologies LLC filed Critical Trilink Biotechnologies LLC
Publication of DE602007013223D1 publication Critical patent/DE602007013223D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE602007013223T 2006-06-01 2007-05-17 Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation Active DE602007013223D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81066506P 2006-06-01 2006-06-01
PCT/US2007/011950 WO2007139723A1 (en) 2006-06-01 2007-05-17 Chemicallymodified oligonucleotide primers for nucleic acid amplification

Publications (1)

Publication Number Publication Date
DE602007013223D1 true DE602007013223D1 (de) 2011-04-28

Family

ID=38778971

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602007013223T Active DE602007013223D1 (de) 2006-06-01 2007-05-17 Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation

Country Status (11)

Country Link
US (1) US8361753B2 (de)
EP (1) EP2032714B1 (de)
JP (1) JP5558811B2 (de)
CN (1) CN101517091B (de)
AT (1) ATE502122T1 (de)
AU (1) AU2007268075C1 (de)
CA (1) CA2653841C (de)
DE (1) DE602007013223D1 (de)
ES (1) ES2360738T3 (de)
HK (1) HK1129045A1 (de)
WO (1) WO2007139723A1 (de)

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US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2279263T3 (da) 2008-04-30 2013-11-11 Integrated Dna Tech Inc Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer
JP5689413B2 (ja) 2008-05-21 2015-03-25 ライニッシュ フリードリッヒ−ウィルヘルムズ−ユニバーシタット ボン 平滑末端を有する５’三リン酸オリゴヌクレオチドおよびその使用
WO2009151921A1 (en) 2008-05-27 2009-12-17 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
CN101638685B (zh) * 2008-07-29 2013-01-09 杭州优思达生物技术有限公司 交叉引物扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用
WO2010080691A1 (en) * 2009-01-06 2010-07-15 Qimin You Cross priming amplification of target nucleic acids
WO2011045744A2 (en) * 2009-10-13 2011-04-21 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
WO2012094343A1 (en) * 2011-01-05 2012-07-12 Trilink Biotechnologies Chemically substituted thermosensitive probes and cofactors for hot start ligation
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EA202090338A1 (ru) * 2011-11-18 2021-04-30 Сарепта Терапьютикс, Инк. Функционально-модифицированные олигонуклеотиды и их субъединицы
US9353393B2 (en) 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US9150896B2 (en) * 2012-09-06 2015-10-06 Illumina, Inc. Nucleotides and primers with removable blocking groups
EP2712870A1 (de) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Neuartige RIG-I-Liganden und Herstellungsverfahren dafür
SG10201806000YA (en) 2013-10-07 2018-08-30 Mitsui Chemicals Inc Primer set for pcr for bacterial dna amplification, kit for detection and/or identification of bacterial species, and, method of detection and/or identification of bacterial species
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
CN117025590A (zh) 2015-09-21 2023-11-10 垂林克生物技术有限公司 用于合成5’-加帽rna的组合物和方法
US11371087B2 (en) 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
EP3486321A4 (de) * 2016-06-23 2020-01-15 Riken T-zell-rezeptor und b-zell-rezeptor-repertoire-analysesystem unter verwendung einer template-switching-polymerasekettenraktion mit einstufiger reverser transkription
SG11202002860VA (en) 2017-10-12 2020-04-29 Mitsui Chemicals Inc mecA GENE AMPLIFICATION PRIMER PAIR, mecA GENE DETECTION KIT AND mecA GENE DETECTION METHOD
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
US20210301324A1 (en) 2018-08-24 2021-09-30 Repertoire Genesis Incorporation Method for analyzing functional subunit pair gene of t cell receptor and b cell receptor
CN109680342B (zh) * 2018-12-18 2021-09-28 上海药明康德新药开发有限公司 DNA编码化合物库中On-DNA芳硝基化合物还原成On-DNA芳胺化合物的方法
WO2020214589A1 (en) * 2019-04-17 2020-10-22 Sena Research, Incorporated Modified nucleotides and methods for dna and rna polymerization and sequencing
US11739359B2 (en) 2020-05-01 2023-08-29 Microsoft Technology Licensing, Llc Universal template strands for enzymatic polynucleotide synthesis
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
WO2023147352A1 (en) 2022-01-27 2023-08-03 Trilink Biotechnologies, Llc Trinucleotide cap analogs and methods of use thereof

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
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US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
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US6965041B1 (en) * 1999-03-24 2005-11-15 The United States Of America As Represented By The Department Of Health And Human Services N-acylphosphoramidites and their use in oligonucleotide synthesis
US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
WO2002018616A1 (en) 2000-09-01 2002-03-07 Hitachi Chemical Co., Ltd. Adjusting the efficiency of nucleic acid template amplification by attenuated pcr with template-mimic oligonucleotides
US6743905B2 (en) * 2001-04-16 2004-06-01 Applera Corporation Mobility-modified nucleobase polymers and methods of using same
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB2416352B (en) 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
EP1841888A4 (de) * 2005-01-04 2009-05-27 Stratagene California Heissstart-polymerasereaktion mittels eines thermolabilen blockers
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Also Published As

Publication number Publication date
WO2007139723A8 (en) 2009-12-17
AU2007268075A1 (en) 2007-12-06
CA2653841C (en) 2016-07-19
EP2032714B1 (de) 2011-03-16
AU2007268075C1 (en) 2013-03-07
ATE502122T1 (de) 2011-04-15
EP2032714A4 (de) 2009-12-23
JP2009538621A (ja) 2009-11-12
CN101517091A (zh) 2009-08-26
JP5558811B2 (ja) 2014-07-23
US20070281308A1 (en) 2007-12-06
EP2032714A1 (de) 2009-03-11
HK1129045A1 (en) 2009-11-13
ES2360738T3 (es) 2011-06-08
AU2007268075B2 (en) 2012-09-13
CN101517091B (zh) 2013-02-13
WO2007139723A1 (en) 2007-12-06
US8361753B2 (en) 2013-01-29
CA2653841A1 (en) 2007-12-06

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