WO2009102896A3 - Isothermal nucleic acid amplification methods and compositions - Google Patents
Isothermal nucleic acid amplification methods and compositions Download PDFInfo
- Publication number
- WO2009102896A3 WO2009102896A3 PCT/US2009/033964 US2009033964W WO2009102896A3 WO 2009102896 A3 WO2009102896 A3 WO 2009102896A3 US 2009033964 W US2009033964 W US 2009033964W WO 2009102896 A3 WO2009102896 A3 WO 2009102896A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- compositions
- nucleic acid
- target
- acid amplification
- amplification methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Abstract
Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3' and or 5' sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09709858A EP2250283A2 (en) | 2008-02-12 | 2009-02-12 | Isothermal nucleic acid amplification methods and compositions |
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2814608P | 2008-02-12 | 2008-02-12 | |
US61/028,146 | 2008-02-12 | ||
US3869608P | 2008-03-21 | 2008-03-21 | |
US61/038,696 | 2008-03-21 | ||
US7499108P | 2008-06-23 | 2008-06-23 | |
US61/074,991 | 2008-06-23 | ||
US8581108P | 2008-08-01 | 2008-08-01 | |
US61/085,811 | 2008-08-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009102896A2 WO2009102896A2 (en) | 2009-08-20 |
WO2009102896A3 true WO2009102896A3 (en) | 2009-11-19 |
Family
ID=40957495
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/033936 WO2009102878A2 (en) | 2008-02-12 | 2009-02-12 | Method for archiving and clonal expansion |
PCT/US2009/033964 WO2009102896A2 (en) | 2008-02-12 | 2009-02-12 | Isothermal nucleic acid amplification methods and compositions |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/033936 WO2009102878A2 (en) | 2008-02-12 | 2009-02-12 | Method for archiving and clonal expansion |
Country Status (2)
Country | Link |
---|---|
EP (2) | EP2247727A4 (en) |
WO (2) | WO2009102878A2 (en) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102439177B (en) | 2009-04-02 | 2014-10-01 | 弗卢伊蒂格姆公司 | Multi-primer amplification method for barcoding of target nucleic acids |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
EP2369325A1 (en) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Array analysis for online detection |
KR101726238B1 (en) * | 2010-10-15 | 2017-04-12 | (주) 하임바이오텍 | Method for non-specifically extending base sequences of target polynucleotide, primer composition for performing the method and kit for improving sensitivity of detecting target polynucleotide |
TWI435935B (en) * | 2010-12-06 | 2014-05-01 | Univ Nat Cheng Kung | Kit and method for rapidly detecting a target nucleic acid fragment |
EP2652148B1 (en) * | 2010-12-17 | 2016-11-30 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
WO2013112923A1 (en) | 2012-01-26 | 2013-08-01 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
JP6445426B2 (en) | 2012-05-10 | 2018-12-26 | ザ ジェネラル ホスピタル コーポレイション | Method for determining nucleotide sequence |
CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
EP3068883B1 (en) | 2013-11-13 | 2020-04-29 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
WO2015112974A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
GB201410646D0 (en) * | 2014-06-14 | 2014-07-30 | Illumina Cambridge Ltd | Methods of increasing sequencing accuracy |
SG11201700891SA (en) | 2014-08-06 | 2017-03-30 | Nugen Technologies Inc | Digital measurements from targeted sequencing |
WO2016149837A1 (en) * | 2015-03-25 | 2016-09-29 | Axela Inc. | Solid phase nucleic acid target capture and replication using strand displacing polymerases |
EP3390658B1 (en) | 2015-12-16 | 2022-08-03 | Standard BioTools Inc. | High-level multiplex amplification |
CN105624302B (en) * | 2016-02-04 | 2019-10-18 | 浙江大学 | Composite label and its application for arthropod bio-diversity high-flux sequence |
AU2017328950B2 (en) | 2016-09-15 | 2023-09-14 | Archerdx, Llc | Methods of nucleic acid sample preparation |
CA3037190A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
EP3927717A4 (en) * | 2019-02-19 | 2022-12-21 | Pioneer Biolabs, LLC | Guide strand library construction and methods of use thereof |
CN111662962B (en) * | 2020-06-09 | 2023-06-23 | 珠海市坤元科技有限公司 | Nucleic acid isothermal amplification method adopting bidirectional strand replacement loop circulation |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6946251B2 (en) * | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
WO2007030759A2 (en) * | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
AR031640A1 (en) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT |
AU2004254552B2 (en) * | 2003-01-29 | 2008-04-24 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
JP2009506788A (en) * | 2005-09-06 | 2009-02-19 | ジェン−プローブ・インコーポレーテッド | Methods, compositions and kits for isothermal amplification of nucleic acids |
-
2009
- 2009-02-12 EP EP09711405A patent/EP2247727A4/en not_active Withdrawn
- 2009-02-12 WO PCT/US2009/033936 patent/WO2009102878A2/en active Application Filing
- 2009-02-12 WO PCT/US2009/033964 patent/WO2009102896A2/en active Application Filing
- 2009-02-12 EP EP09709858A patent/EP2250283A2/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6946251B2 (en) * | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
WO2007030759A2 (en) * | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
Non-Patent Citations (1)
Title |
---|
KURN, N. ET AL.: "Novel isothermal, linear nucleic acid amplification systems for highly multiplexed applications.", CLIN. CHEM., vol. 51, no. 10, 25 August 2005 (2005-08-25), pages 1973 - 1981, XP002420278 * |
Also Published As
Publication number | Publication date |
---|---|
EP2250283A2 (en) | 2010-11-17 |
WO2009102896A2 (en) | 2009-08-20 |
WO2009102878A2 (en) | 2009-08-20 |
EP2247727A4 (en) | 2011-08-03 |
WO2009102878A3 (en) | 2010-01-21 |
EP2247727A2 (en) | 2010-11-10 |
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