EP3927717A4 - Guide strand library construction and methods of use thereof - Google Patents

Guide strand library construction and methods of use thereof Download PDF

Info

Publication number
EP3927717A4
EP3927717A4 EP20759378.1A EP20759378A EP3927717A4 EP 3927717 A4 EP3927717 A4 EP 3927717A4 EP 20759378 A EP20759378 A EP 20759378A EP 3927717 A4 EP3927717 A4 EP 3927717A4
Authority
EP
European Patent Office
Prior art keywords
methods
library construction
guide strand
strand library
guide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20759378.1A
Other languages
German (de)
French (fr)
Other versions
EP3927717A1 (en
Inventor
Joshua Yates
Jonathon Hill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Biolabs LLC
Original Assignee
Pioneer Biolabs LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Biolabs LLC filed Critical Pioneer Biolabs LLC
Publication of EP3927717A1 publication Critical patent/EP3927717A1/en
Publication of EP3927717A4 publication Critical patent/EP3927717A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
EP20759378.1A 2019-02-19 2020-02-18 Guide strand library construction and methods of use thereof Withdrawn EP3927717A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962807679P 2019-02-19 2019-02-19
US201962902601P 2019-09-19 2019-09-19
PCT/US2020/018701 WO2020172199A1 (en) 2019-02-19 2020-02-18 Guide strand library construction and methods of use thereof

Publications (2)

Publication Number Publication Date
EP3927717A1 EP3927717A1 (en) 2021-12-29
EP3927717A4 true EP3927717A4 (en) 2022-12-21

Family

ID=72145068

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20759378.1A Withdrawn EP3927717A4 (en) 2019-02-19 2020-02-18 Guide strand library construction and methods of use thereof

Country Status (2)

Country Link
EP (1) EP3927717A4 (en)
WO (1) WO2020172199A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202114206D0 (en) * 2021-10-04 2021-11-17 Genome Res Ltd Novel method
CN114277447A (en) * 2021-12-21 2022-04-05 翌圣生物科技(上海)股份有限公司 Preparation method of target sequence random sgRNA full-coverage group
CN114293264A (en) * 2021-12-21 2022-04-08 翌圣生物科技(上海)股份有限公司 Preparation method of enzyme method target sequence random sgRNA library

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011133695A2 (en) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
US20180100147A1 (en) * 2016-10-06 2018-04-12 Brigham Young University Methods and compositions for generating crispr guide rna libraries

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
EP2247727A4 (en) * 2008-02-12 2011-08-03 Nugen Technologies Inc Method for archiving and clonal expansion
RU2013129999A (en) * 2010-12-02 2015-01-10 Дайити Санкио Компани, Лимитед MODIFIED SINGLE-CHAIN POLYNUCLEOTIDE
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US10253321B2 (en) * 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
EP3334823A4 (en) * 2015-06-05 2019-05-22 The Regents of The University of California Methods and compositions for generating crispr/cas guide rnas
WO2020033585A1 (en) * 2018-08-07 2020-02-13 The Broad Institute, Inc. Methods for combinatorial screening and use of therapeutic targets thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011133695A2 (en) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
US20180100147A1 (en) * 2016-10-06 2018-04-12 Brigham Young University Methods and compositions for generating crispr guide rna libraries

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DON YATES JOSHUA: "A CRISPR/Cas9 Tissue Specific Forward Genetic Screening Method in Danio rerio Method in Danio rerio", 9 April 2019 (2019-04-09), XP055977331, Retrieved from the Internet <URL:https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=10282&context=etd> [retrieved on 20221102] *
KARLSSON KASPER ET AL: "Amplification-free sequencing of cell-free DNA for prenatal non-invasive diagnosis of chromosomal aberrations", GENOMICS, vol. 105, no. 3, 24 December 2014 (2014-12-24), US, pages 150 - 158, XP055977655, ISSN: 0888-7543, DOI: 10.1016/j.ygeno.2014.12.005 *
See also references of WO2020172199A1 *

Also Published As

Publication number Publication date
WO2020172199A1 (en) 2020-08-27
EP3927717A1 (en) 2021-12-29

Similar Documents

Publication Publication Date Title
EP3744844A4 (en) Extended single guide rna and use thereof
EP3968999A4 (en) Fgfr inhibitors and methods of use thereof
EP3927717A4 (en) Guide strand library construction and methods of use thereof
EP4051788A4 (en) Type v crispr-cas base editors and methods of use thereof
EP3972991A4 (en) Nr4a super-repressors and methods of use thereof
EP4001305A4 (en) Anti-tau antibody and use of same
EP3962481A4 (en) Kcnt1 inhibitors and methods of use
EP3962479A4 (en) Kcnt1 inhibitors and methods of use
EP3969439A4 (en) Acss2 inhibitors and methods of use thereof
EP3886853A4 (en) Diarylhydantoin compounds and methods of use thereof
EP3986894A4 (en) Ectonucleotidase inhibitors and methods of use thereof
EP3752166A4 (en) Trialkyne linking agents and methods of use
EP3846702A4 (en) Surgical guide and methods of use
EP3983809A4 (en) Biothiol-activatable probe and method of use
EP3917543A4 (en) Enhanced nitrate compositions and methods of use
EP3952721A4 (en) Endoscope and method of use
EP3972646A4 (en) Apohemoglobin-haptoglobin complexes and methods of using thereof
EP3959197A4 (en) Papd5 inhibitors and methods of use thereof
EP3761989A4 (en) Imidazodiazepinediones and methods of use thereof
EP4061848A4 (en) Anti-ror-2 antibodies and methods of use
EP4054330A4 (en) A composition and related methods of manufacture and use
EP4017568A4 (en) Catheters and methods of manufacture and use
EP3946461A4 (en) Xyloglucan-containing prodrugs and methods of manufacture and use thereof
GB201902716D0 (en) Fibre optic cable, methods of manufacture and use thereof
EP3808449A4 (en) Catalyst and method of use thereof

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20210915

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20221117

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6806 20180101ALI20221111BHEP

Ipc: C12Q 1/68 20180101ALI20221111BHEP

Ipc: C12P 19/34 20060101ALI20221111BHEP

Ipc: C12N 15/11 20060101ALI20221111BHEP

Ipc: C12N 15/10 20060101ALI20221111BHEP

Ipc: C07H 21/00 20060101AFI20221111BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20230617