EP3927717A4 - Construction de banques de brins guides et procédés d'utilisation associés - Google Patents

Construction de banques de brins guides et procédés d'utilisation associés Download PDF

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Publication number
EP3927717A4
EP3927717A4 EP20759378.1A EP20759378A EP3927717A4 EP 3927717 A4 EP3927717 A4 EP 3927717A4 EP 20759378 A EP20759378 A EP 20759378A EP 3927717 A4 EP3927717 A4 EP 3927717A4
Authority
EP
European Patent Office
Prior art keywords
methods
library construction
guide strand
strand library
guide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20759378.1A
Other languages
German (de)
English (en)
Other versions
EP3927717A1 (fr
Inventor
Joshua Yates
Jonathon Hill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Biolabs LLC
Original Assignee
Pioneer Biolabs LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Biolabs LLC filed Critical Pioneer Biolabs LLC
Publication of EP3927717A1 publication Critical patent/EP3927717A1/fr
Publication of EP3927717A4 publication Critical patent/EP3927717A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
EP20759378.1A 2019-02-19 2020-02-18 Construction de banques de brins guides et procédés d'utilisation associés Withdrawn EP3927717A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962807679P 2019-02-19 2019-02-19
US201962902601P 2019-09-19 2019-09-19
PCT/US2020/018701 WO2020172199A1 (fr) 2019-02-19 2020-02-18 Construction de banques de brins guides et procédés d'utilisation associés

Publications (2)

Publication Number Publication Date
EP3927717A1 EP3927717A1 (fr) 2021-12-29
EP3927717A4 true EP3927717A4 (fr) 2022-12-21

Family

ID=72145068

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20759378.1A Withdrawn EP3927717A4 (fr) 2019-02-19 2020-02-18 Construction de banques de brins guides et procédés d'utilisation associés

Country Status (2)

Country Link
EP (1) EP3927717A4 (fr)
WO (1) WO2020172199A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202114206D0 (en) * 2021-10-04 2021-11-17 Genome Res Ltd Novel method
CN114277447A (zh) * 2021-12-21 2022-04-05 翌圣生物科技(上海)股份有限公司 靶序列随机sgRNA全覆盖组的制备方法
CN114293264A (zh) * 2021-12-21 2022-04-08 翌圣生物科技(上海)股份有限公司 酶法靶序列随机sgRNA文库的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011133695A2 (fr) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Matériaux et procédés de fractionnement de l'acide nucléique par piégeage en phase solide et détachement enzymatique
US20180100147A1 (en) * 2016-10-06 2018-04-12 Brigham Young University Methods and compositions for generating crispr guide rna libraries

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0652971A4 (fr) * 1992-07-28 1998-12-02 Hitachi Chemical Co Ltd Systeme de detection de gene.
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
EP2250283A2 (fr) * 2008-02-12 2010-11-17 Nugen Technologies, Inc. Procédés et composition pour amplification isotherme d'acide nucléique
EP2647713B1 (fr) * 2010-12-02 2018-02-21 Daiichi Sankyo Company, Limited Polynucléotide modifié à simple brin
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US10253321B2 (en) * 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
EP3334823B1 (fr) * 2015-06-05 2024-05-22 The Regents of The University of California Procédé et kit pour générer des arn guide crispr/cas
US20210308171A1 (en) * 2018-08-07 2021-10-07 The Broad Institute, Inc. Methods for combinatorial screening and use of therapeutic targets thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011133695A2 (fr) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Matériaux et procédés de fractionnement de l'acide nucléique par piégeage en phase solide et détachement enzymatique
US20180100147A1 (en) * 2016-10-06 2018-04-12 Brigham Young University Methods and compositions for generating crispr guide rna libraries

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DON YATES JOSHUA: "A CRISPR/Cas9 Tissue Specific Forward Genetic Screening Method in Danio rerio Method in Danio rerio", 9 April 2019 (2019-04-09), XP055977331, Retrieved from the Internet <URL:https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=10282&context=etd> [retrieved on 20221102] *
KARLSSON KASPER ET AL: "Amplification-free sequencing of cell-free DNA for prenatal non-invasive diagnosis of chromosomal aberrations", GENOMICS, vol. 105, no. 3, 24 December 2014 (2014-12-24), US, pages 150 - 158, XP055977655, ISSN: 0888-7543, DOI: 10.1016/j.ygeno.2014.12.005 *
See also references of WO2020172199A1 *

Also Published As

Publication number Publication date
EP3927717A1 (fr) 2021-12-29
WO2020172199A1 (fr) 2020-08-27

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