CY1109285T1 - Ανακτηση toy dna - Google Patents

Ανακτηση toy dna

Info

Publication number
CY1109285T1
CY1109285T1 CY20091100852T CY091100852T CY1109285T1 CY 1109285 T1 CY1109285 T1 CY 1109285T1 CY 20091100852 T CY20091100852 T CY 20091100852T CY 091100852 T CY091100852 T CY 091100852T CY 1109285 T1 CY1109285 T1 CY 1109285T1
Authority
CY
Cyprus
Prior art keywords
mrna
cdna
methods
recovery
region
Prior art date
Application number
CY20091100852T
Other languages
English (en)
Inventor
Mingyue He
Alison M Jackson
Original Assignee
Crescendo Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0306305A external-priority patent/GB0306305D0/en
Priority claimed from GB0311351A external-priority patent/GB0311351D0/en
Application filed by Crescendo Biologics Ltd filed Critical Crescendo Biologics Ltd
Publication of CY1109285T1 publication Critical patent/CY1109285T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Throttle Valves Provided In The Intake System Or In The Exhaust System (AREA)
  • Valve Device For Special Equipments (AREA)

Abstract

Η εφεύρεση προσφέρει κάποια μέθοδο για την ανάκτηση cDNA από mRNA, η οποία περιλαμβάνει αντίστροφη μεταγραφή (RT) του mRNA χρησιμοποιώντας κάποιον εκκινητή RT που περιλαμβάνει κάποια αλληλουχία βασισμένη στην 5' συναινετική περιοχή του mRNA η οποία είναι πανομοιότυπη ή παρόμοια με την 5' συναινετική περιοχή του mRNA και η οποία περιλαμβάνει κάποια αλληλουχία ικανή να υβριδοποιείται ειδικά με την 3' περιοχή του mRNA, ακολουθούμενη από αλυσιδωτή αντίδραση πολυμεράσης (PCR) χρησιμοποιώντας ένα μόνο εκκινητή για την δημιουργία ss cDNA, ds cDNA και την ενίσχυση του cDNA. Παρέχονται επίσης οι εκκινητές που χρησιμοποιούνται στις μεθόδους της εφεύρεσης και τα κιτ για την πραγματοποίηση των μεθόδων της εφεύρεσης. Οι μέθοδοι της εφεύρεσης μπορεί να είναι πλήρως ή μερικώς αυτοματοποιημένες.
CY20091100852T 2003-03-19 2009-08-11 Ανακτηση toy dna CY1109285T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0306305A GB0306305D0 (en) 2003-03-19 2003-03-19 Recovery of DNA
GB0311351A GB0311351D0 (en) 2003-05-16 2003-05-16 Activity-directed in vitro protein cloning and identification
EP04721937A EP1604044B1 (en) 2003-03-19 2004-03-19 Recovery of dna

Publications (1)

Publication Number Publication Date
CY1109285T1 true CY1109285T1 (el) 2014-07-02

Family

ID=33031411

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100852T CY1109285T1 (el) 2003-03-19 2009-08-11 Ανακτηση toy dna

Country Status (9)

Country Link
US (1) US7572577B2 (el)
EP (1) EP1604044B1 (el)
AT (1) ATE431430T1 (el)
CY (1) CY1109285T1 (el)
DE (1) DE602004021094D1 (el)
DK (1) DK1604044T3 (el)
ES (1) ES2326649T3 (el)
PT (1) PT1604044E (el)
WO (2) WO2004083428A1 (el)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3629022A1 (en) 2008-07-25 2020-04-01 Richard W. Wagner Protein screening methods
CN109476695A (zh) * 2016-06-27 2019-03-15 丹娜法伯癌症研究院 用于测定rna翻译速率的方法
CN110904135B (zh) * 2019-12-30 2021-08-13 苏州珀罗汀生物技术有限公司 蛋白质基础表达体系、合成系统及制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
CA2080305A1 (en) * 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
ES2209145T5 (es) * 1997-05-28 2009-10-30 Crescendo Biologics Ltd. Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas.
US6207424B1 (en) * 1999-11-23 2001-03-27 Maxim Biotech, Inc. Self-primed amplification system
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA

Also Published As

Publication number Publication date
US7572577B2 (en) 2009-08-11
DE602004021094D1 (de) 2009-06-25
DK1604044T3 (da) 2009-08-31
EP1604044A1 (en) 2005-12-14
ATE431430T1 (de) 2009-05-15
WO2004083461A1 (en) 2004-09-30
WO2004083461A9 (en) 2004-12-29
ES2326649T3 (es) 2009-10-16
WO2004083428A1 (en) 2004-09-30
EP1604044B1 (en) 2009-05-13
US20060281086A1 (en) 2006-12-14
PT1604044E (pt) 2009-08-05

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