ATE476523T1 - Verfahren zur genomamplifikation - Google Patents
Verfahren zur genomamplifikationInfo
- Publication number
- ATE476523T1 ATE476523T1 AT04735461T AT04735461T ATE476523T1 AT E476523 T1 ATE476523 T1 AT E476523T1 AT 04735461 T AT04735461 T AT 04735461T AT 04735461 T AT04735461 T AT 04735461T AT E476523 T1 ATE476523 T1 AT E476523T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- modified dna
- genome amplification
- providing
- polymerase
- Prior art date
Links
- 238000013412 genome amplification Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229940104302 cytosine Drugs 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2003903039A AU2003903039A0 (en) | 2003-06-17 | 2003-06-17 | Whole genome amplification |
| AU2003903034A AU2003903034A0 (en) | 2003-06-17 | 2003-06-17 | Epigenomics analysis - genome amplification |
| PCT/AU2004/000722 WO2004111266A1 (en) | 2003-06-17 | 2004-05-31 | Methods for genome amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE476523T1 true ATE476523T1 (de) | 2010-08-15 |
Family
ID=33553188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04735461T ATE476523T1 (de) | 2003-06-17 | 2004-05-31 | Verfahren zur genomamplifikation |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7799525B2 (de) |
| EP (1) | EP1641936B1 (de) |
| AT (1) | ATE476523T1 (de) |
| DE (1) | DE602004028478D1 (de) |
| WO (1) | WO2004111266A1 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7288373B2 (en) * | 2003-05-02 | 2007-10-30 | Human Genetic Signatures Pty Ltd. | Treatment of methylated nucleic acid |
| ATE476523T1 (de) | 2003-06-17 | 2010-08-15 | Human Genetic Signatures Pty | Verfahren zur genomamplifikation |
| CN1863927B (zh) * | 2003-09-04 | 2010-08-11 | 人类遗传标记控股有限公司 | 核酸检测分析 |
| US8168777B2 (en) | 2004-04-29 | 2012-05-01 | Human Genetic Signatures Pty. Ltd. | Bisulphite reagent treatment of nucleic acid |
| DE602005022740D1 (de) * | 2004-09-10 | 2010-09-16 | Human Genetic Signatures Pty | Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn) |
| ES2399054T3 (es) * | 2004-12-03 | 2013-03-25 | Human Genetic Signatures Pty Ltd | Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas |
| AU2006251866B2 (en) * | 2005-05-26 | 2007-11-29 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| US20060292578A1 (en) * | 2005-06-28 | 2006-12-28 | Weidong Zheng | DNA polymerase blends and mutant DNA polymerases |
| ATE531820T1 (de) * | 2005-09-14 | 2011-11-15 | Human Genetic Signatures Pty | Gesundheitszustandstest |
| EP1994182B1 (de) * | 2006-03-15 | 2019-05-29 | Siemens Healthcare Diagnostics Inc. | Abgleit-nucleobase-analoga |
| US20080050738A1 (en) * | 2006-05-31 | 2008-02-28 | Human Genetic Signatures Pty Ltd. | Detection of target nucleic acid |
| JP2010521142A (ja) * | 2007-03-16 | 2010-06-24 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | 遺伝子発現アッセイ |
| AU2008329549B2 (en) | 2007-11-27 | 2013-08-22 | Human Genetic Signatures Pty Ltd | Enzymes for amplification and copying bisulphite modified nucleic acids |
| EP2215035A4 (de) * | 2007-12-05 | 2011-12-14 | Human Genetic Signatures Pty | Bisulfitbehandlung von rna |
| CA2709632A1 (en) * | 2007-12-20 | 2009-07-02 | Human Genetic Signatures Pty Ltd | Elimination of contaminants associated with nucleic acid amplification |
| JP5357893B2 (ja) * | 2007-12-27 | 2013-12-04 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 迅速超長鎖pcrのための単一酵素系 |
| WO2009121012A1 (en) * | 2008-03-27 | 2009-10-01 | Greene, Tweed Of Delaware, Inc. | Inert substrate-bonded fluoroelastometer components and related methods |
| EP2297343A1 (de) * | 2008-05-16 | 2011-03-23 | New England Biolabs, Inc. | Enzymreagentien zur amplifikation von polynukleotiden in gegenwart von inhibitoren |
| CN102533960B (zh) * | 2010-12-31 | 2014-04-30 | 深圳华大基因科技有限公司 | 一种单细胞基因组分析方法及试剂盒 |
| DK2753710T3 (en) | 2011-09-07 | 2017-02-06 | Human Genetic Signatures Pty Ltd | MOLECULAR DETECTION ASSAY |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| EP0625213B1 (de) * | 1992-10-09 | 1999-12-29 | VYSIS, Inc. | Analytisches verfahren |
| US5473060A (en) | 1993-07-02 | 1995-12-05 | Lynx Therapeutics, Inc. | Oligonucleotide clamps having diagnostic applications |
| US6156501A (en) * | 1993-10-26 | 2000-12-05 | Affymetrix, Inc. | Arrays of modified nucleic acid probes and methods of use |
| EP0754240B1 (de) | 1994-02-07 | 2003-08-20 | Beckman Coulter, Inc. | Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| SE9601676D0 (sv) | 1996-04-30 | 1996-04-30 | Ulf Landegren | Improved probing of specific mucleic acids |
| JP4468488B2 (ja) | 1996-05-29 | 2010-05-26 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出 |
| US6017704A (en) * | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| DE69720283T2 (de) | 1996-12-30 | 2004-01-29 | Univ Johns Hopkins Med | Bestimmung der krebsanfälligkeit eines organs oder gewebes durch bestimmung seiner zellprägung |
| DE69829760T3 (de) | 1997-09-12 | 2016-04-14 | Exiqon A/S | Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga |
| DE19754482A1 (de) | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| GB9806253D0 (en) | 1998-03-25 | 1998-05-20 | Tepnel Medical Ltd | Amplification of nucleic acids |
| DE19905082C1 (de) | 1999-01-29 | 2000-05-18 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben |
| US6410231B1 (en) | 1999-02-26 | 2002-06-25 | Incyte Genomics, Inc. | SNP detection |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| DE19935749C2 (de) | 1999-07-28 | 2003-06-26 | Epigenomics Ag | Verfahren zur Chrakterisierung von Nukleinsäurefragmenten |
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| WO2001036669A2 (de) * | 1999-11-12 | 2001-05-25 | Epigenomics Ag | Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen |
| DE19957827C2 (de) | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| DE19959691A1 (de) | 1999-12-06 | 2001-08-16 | Epigenomics Ag | Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA |
| EP1130113A1 (de) | 2000-02-15 | 2001-09-05 | Johannes Petrus Schouten | Amplifizierungsassay abhängig von multiplex Ligation |
| DE10010281B4 (de) * | 2000-02-25 | 2005-03-10 | Epigenomics Ag | Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| US20040076956A1 (en) | 2000-04-06 | 2004-04-22 | Alexander Olek | Diagnosis of diseases associated with dna repair |
| AUPR142500A0 (en) | 2000-11-13 | 2000-12-07 | Human Genetic Signatures Pty Ltd | A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences |
| DE10061348C2 (de) * | 2000-12-06 | 2002-10-24 | Epigenomics Ag | Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA |
| US20020142397A1 (en) * | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
| EP1359219A4 (de) * | 2001-02-06 | 2004-09-22 | Takara Bio Inc | Amplifizierte nukleinsäuren und immobilisierte produkte davon |
| DE10112515B4 (de) | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| GB0113118D0 (en) | 2001-05-31 | 2001-07-18 | Intercytex Ltd | Stem Cells |
| JP2004535815A (ja) | 2001-07-15 | 2004-12-02 | ケック グラデュエイト インスティテュート | ニック形成剤を用いた核酸フラグメントの増幅 |
| US7056671B2 (en) * | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
| CA2467814A1 (en) | 2001-12-03 | 2003-06-12 | Illumina, Inc. | Multiplexed methylation detection methods |
| EP1319718A1 (de) | 2001-12-14 | 2003-06-18 | Keygene N.V. | Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen |
| CN1653079B (zh) | 2001-12-18 | 2010-06-16 | 人类遗传标记控股有限公司 | 包括嵌入剂的假核苷酸 |
| US20060014144A1 (en) * | 2001-12-18 | 2006-01-19 | Christensen Ulf B | Pseudonucleotide comprising an intercalator |
| US7932070B2 (en) * | 2001-12-21 | 2011-04-26 | Agilent Technologies, Inc. | High fidelity DNA polymerase compositions and uses therefor |
| US6960436B2 (en) * | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
| DE10236406C1 (de) | 2002-08-02 | 2003-12-24 | Epigenomics Ag | Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität |
| SE0202867D0 (sv) * | 2002-09-27 | 2002-09-27 | Pyrosequencing Ab | New sequencing method |
| AU2002951899A0 (en) * | 2002-10-04 | 2002-10-24 | Macquarie University | Random drift mutagenesis |
| AU2003900368A0 (en) | 2003-01-24 | 2003-02-13 | Human Genetic Signatures Pty Ltd | Assay for nucleic acid molecules |
| US20040203004A1 (en) | 2003-04-10 | 2004-10-14 | Bernard Hans Ulrich | Diagnostic apparatus and method |
| US7288373B2 (en) * | 2003-05-02 | 2007-10-30 | Human Genetic Signatures Pty Ltd. | Treatment of methylated nucleic acid |
| ATE476523T1 (de) | 2003-06-17 | 2010-08-15 | Human Genetic Signatures Pty | Verfahren zur genomamplifikation |
| DE10331107B3 (de) | 2003-07-04 | 2004-12-02 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA mittels Cytidin-Deaminasen |
| US7368239B2 (en) | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| CN1863927B (zh) * | 2003-09-04 | 2010-08-11 | 人类遗传标记控股有限公司 | 核酸检测分析 |
| WO2005054502A1 (en) * | 2003-12-02 | 2005-06-16 | Roche Diagnostics Gmbh | Improved method for bisulfite treatment |
| ATE458044T1 (de) | 2003-12-10 | 2010-03-15 | Takara Bio Inc | Verfahren zur nukleinsäureamplifikation |
| WO2005090607A1 (en) * | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| US8168777B2 (en) * | 2004-04-29 | 2012-05-01 | Human Genetic Signatures Pty. Ltd. | Bisulphite reagent treatment of nucleic acid |
| US7361465B2 (en) * | 2004-09-07 | 2008-04-22 | Applera Corporation | Methods and compositions for tailing and amplifying RNA |
| DE602005022740D1 (de) * | 2004-09-10 | 2010-09-16 | Human Genetic Signatures Pty | Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn) |
| ES2399054T3 (es) | 2004-12-03 | 2013-03-25 | Human Genetic Signatures Pty Ltd | Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas |
| CN101142323A (zh) * | 2004-12-23 | 2008-03-12 | 人类遗传标记控股有限公司 | 人类乳头瘤病毒的检测 |
| ES2546848T3 (es) * | 2006-03-10 | 2015-09-29 | Epigenomics Ag | Un método para identificar una muestra biológica para el análisis de la metilación |
| US20080050738A1 (en) * | 2006-05-31 | 2008-02-28 | Human Genetic Signatures Pty Ltd. | Detection of target nucleic acid |
-
2004
- 2004-05-31 AT AT04735461T patent/ATE476523T1/de not_active IP Right Cessation
- 2004-05-31 DE DE602004028478T patent/DE602004028478D1/de not_active Expired - Lifetime
- 2004-05-31 US US10/561,029 patent/US7799525B2/en not_active Expired - Fee Related
- 2004-05-31 WO PCT/AU2004/000722 patent/WO2004111266A1/en not_active Ceased
- 2004-05-31 EP EP04735461A patent/EP1641936B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7799525B2 (en) | 2010-09-21 |
| WO2004111266A1 (en) | 2004-12-23 |
| EP1641936B1 (de) | 2010-08-04 |
| DE602004028478D1 (de) | 2010-09-16 |
| EP1641936A1 (de) | 2006-04-05 |
| US20070178457A1 (en) | 2007-08-02 |
| EP1641936A4 (de) | 2007-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |