ATE476523T1 - Verfahren zur genomamplifikation - Google Patents

Verfahren zur genomamplifikation

Info

Publication number
ATE476523T1
ATE476523T1 AT04735461T AT04735461T ATE476523T1 AT E476523 T1 ATE476523 T1 AT E476523T1 AT 04735461 T AT04735461 T AT 04735461T AT 04735461 T AT04735461 T AT 04735461T AT E476523 T1 ATE476523 T1 AT E476523T1
Authority
AT
Austria
Prior art keywords
dna
modified dna
genome amplification
providing
polymerase
Prior art date
Application number
AT04735461T
Other languages
English (en)
Inventor
Douglas Millar
Original Assignee
Human Genetic Signatures Pty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2003903039A external-priority patent/AU2003903039A0/en
Priority claimed from AU2003903034A external-priority patent/AU2003903034A0/en
Application filed by Human Genetic Signatures Pty filed Critical Human Genetic Signatures Pty
Application granted granted Critical
Publication of ATE476523T1 publication Critical patent/ATE476523T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
AT04735461T 2003-06-17 2004-05-31 Verfahren zur genomamplifikation ATE476523T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2003903039A AU2003903039A0 (en) 2003-06-17 2003-06-17 Whole genome amplification
AU2003903034A AU2003903034A0 (en) 2003-06-17 2003-06-17 Epigenomics analysis - genome amplification
PCT/AU2004/000722 WO2004111266A1 (en) 2003-06-17 2004-05-31 Methods for genome amplification

Publications (1)

Publication Number Publication Date
ATE476523T1 true ATE476523T1 (de) 2010-08-15

Family

ID=33553188

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04735461T ATE476523T1 (de) 2003-06-17 2004-05-31 Verfahren zur genomamplifikation

Country Status (5)

Country Link
US (1) US7799525B2 (de)
EP (1) EP1641936B1 (de)
AT (1) ATE476523T1 (de)
DE (1) DE602004028478D1 (de)
WO (1) WO2004111266A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
ATE476523T1 (de) 2003-06-17 2010-08-15 Human Genetic Signatures Pty Verfahren zur genomamplifikation
CN1863927B (zh) * 2003-09-04 2010-08-11 人类遗传标记控股有限公司 核酸检测分析
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DE602005022740D1 (de) * 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
ES2399054T3 (es) * 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas
AU2006251866B2 (en) * 2005-05-26 2007-11-29 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
ATE531820T1 (de) * 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
EP1994182B1 (de) * 2006-03-15 2019-05-29 Siemens Healthcare Diagnostics Inc. Abgleit-nucleobase-analoga
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP2010521142A (ja) * 2007-03-16 2010-06-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 遺伝子発現アッセイ
AU2008329549B2 (en) 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
EP2215035A4 (de) * 2007-12-05 2011-12-14 Human Genetic Signatures Pty Bisulfitbehandlung von rna
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
JP5357893B2 (ja) * 2007-12-27 2013-12-04 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 迅速超長鎖pcrのための単一酵素系
WO2009121012A1 (en) * 2008-03-27 2009-10-01 Greene, Tweed Of Delaware, Inc. Inert substrate-bonded fluoroelastometer components and related methods
EP2297343A1 (de) * 2008-05-16 2011-03-23 New England Biolabs, Inc. Enzymreagentien zur amplifikation von polynukleotiden in gegenwart von inhibitoren
CN102533960B (zh) * 2010-12-31 2014-04-30 深圳华大基因科技有限公司 一种单细胞基因组分析方法及试剂盒
DK2753710T3 (en) 2011-09-07 2017-02-06 Human Genetic Signatures Pty Ltd MOLECULAR DETECTION ASSAY

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750338A (en) * 1986-10-23 1998-05-12 Amoco Corporation Target and background capture methods with amplification for affinity assays
EP0625213B1 (de) * 1992-10-09 1999-12-29 VYSIS, Inc. Analytisches verfahren
US5473060A (en) 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications
US6156501A (en) * 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
EP0754240B1 (de) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
SE9601676D0 (sv) 1996-04-30 1996-04-30 Ulf Landegren Improved probing of specific mucleic acids
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
DE69720283T2 (de) 1996-12-30 2004-01-29 Univ Johns Hopkins Med Bestimmung der krebsanfälligkeit eines organs oder gewebes durch bestimmung seiner zellprägung
DE69829760T3 (de) 1997-09-12 2016-04-14 Exiqon A/S Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
GB9806253D0 (en) 1998-03-25 1998-05-20 Tepnel Medical Ltd Amplification of nucleic acids
DE19905082C1 (de) 1999-01-29 2000-05-18 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben
US6410231B1 (en) 1999-02-26 2002-06-25 Incyte Genomics, Inc. SNP detection
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
DE19935749C2 (de) 1999-07-28 2003-06-26 Epigenomics Ag Verfahren zur Chrakterisierung von Nukleinsäurefragmenten
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001036669A2 (de) * 1999-11-12 2001-05-25 Epigenomics Ag Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen
DE19957827C2 (de) 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
DE19959691A1 (de) 1999-12-06 2001-08-16 Epigenomics Ag Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
EP1130113A1 (de) 2000-02-15 2001-09-05 Johannes Petrus Schouten Amplifizierungsassay abhängig von multiplex Ligation
DE10010281B4 (de) * 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
US20040076956A1 (en) 2000-04-06 2004-04-22 Alexander Olek Diagnosis of diseases associated with dna repair
AUPR142500A0 (en) 2000-11-13 2000-12-07 Human Genetic Signatures Pty Ltd A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences
DE10061348C2 (de) * 2000-12-06 2002-10-24 Epigenomics Ag Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
EP1359219A4 (de) * 2001-02-06 2004-09-22 Takara Bio Inc Amplifizierte nukleinsäuren und immobilisierte produkte davon
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
GB0113118D0 (en) 2001-05-31 2001-07-18 Intercytex Ltd Stem Cells
JP2004535815A (ja) 2001-07-15 2004-12-02 ケック グラデュエイト インスティテュート ニック形成剤を用いた核酸フラグメントの増幅
US7056671B2 (en) * 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
CA2467814A1 (en) 2001-12-03 2003-06-12 Illumina, Inc. Multiplexed methylation detection methods
EP1319718A1 (de) 2001-12-14 2003-06-18 Keygene N.V. Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen
CN1653079B (zh) 2001-12-18 2010-06-16 人类遗传标记控股有限公司 包括嵌入剂的假核苷酸
US20060014144A1 (en) * 2001-12-18 2006-01-19 Christensen Ulf B Pseudonucleotide comprising an intercalator
US7932070B2 (en) * 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
DE10236406C1 (de) 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
SE0202867D0 (sv) * 2002-09-27 2002-09-27 Pyrosequencing Ab New sequencing method
AU2002951899A0 (en) * 2002-10-04 2002-10-24 Macquarie University Random drift mutagenesis
AU2003900368A0 (en) 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US20040203004A1 (en) 2003-04-10 2004-10-14 Bernard Hans Ulrich Diagnostic apparatus and method
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
ATE476523T1 (de) 2003-06-17 2010-08-15 Human Genetic Signatures Pty Verfahren zur genomamplifikation
DE10331107B3 (de) 2003-07-04 2004-12-02 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA mittels Cytidin-Deaminasen
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
CN1863927B (zh) * 2003-09-04 2010-08-11 人类遗传标记控股有限公司 核酸检测分析
WO2005054502A1 (en) * 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
ATE458044T1 (de) 2003-12-10 2010-03-15 Takara Bio Inc Verfahren zur nukleinsäureamplifikation
WO2005090607A1 (en) * 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
US8168777B2 (en) * 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
US7361465B2 (en) * 2004-09-07 2008-04-22 Applera Corporation Methods and compositions for tailing and amplifying RNA
DE602005022740D1 (de) * 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
ES2399054T3 (es) 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas
CN101142323A (zh) * 2004-12-23 2008-03-12 人类遗传标记控股有限公司 人类乳头瘤病毒的检测
ES2546848T3 (es) * 2006-03-10 2015-09-29 Epigenomics Ag Un método para identificar una muestra biológica para el análisis de la metilación
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid

Also Published As

Publication number Publication date
US7799525B2 (en) 2010-09-21
WO2004111266A1 (en) 2004-12-23
EP1641936B1 (de) 2010-08-04
DE602004028478D1 (de) 2010-09-16
EP1641936A1 (de) 2006-04-05
US20070178457A1 (en) 2007-08-02
EP1641936A4 (de) 2007-10-24

Similar Documents

Publication Publication Date Title
ATE476523T1 (de) Verfahren zur genomamplifikation
BR0101493B1 (pt) mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes.
NO20021223L (no) Fremgangsmater og sammensetninger for lineaer, isoterm amplifisering av polynukleotidsekvenser
DE60332820D1 (de) Verfahren und mittel zur überwachung und modulation des gen-silencing
DK1370683T3 (da) mRNA-amplifikation
WO2002006533A3 (en) Single primer pcr amplification of rna
DK1499738T3 (da) Recombinase-polymerease-amplifikation
ATE483802T1 (de) Verfahren zur in vitro-rekombination
WO2003008624A3 (en) Nucleic acid amplification using nicking agents
WO2002052031A3 (en) Nucleic acid amplification
WO2003097794A3 (en) Universal-tagged oligonucleotide primers and methods of use
EP2077322A3 (de) RNA-abhängige RNA-Polymerase, Verfahren und Kits zur Amplifikation und/oder Markierung von RNA
WO2003102232A3 (de) Vermehrung von ribonukleinsäuren
MX2007006610A (es) Metodo para simplificar acidos nucleicos microbianos por modificacion quimica de citosinas.
DE60132924D1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
WO2003004703A1 (fr) Processus de production de matrice d'adn et processus de production de proteine dans un systeme de synthese de proteine sans cellule a l'aide du premier processus
ATE497022T1 (de) Verfahren zur amplifikation von nukleinsäure
JP2020182457A5 (de)
ATE340873T1 (de) Verfahren zur durchführung einer subtraktiven hybridisierung
ATE420975T1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
CY1109285T1 (el) Ανακτηση toy dna
DE50312117D1 (de) Verfahren zur Amplifikation genetischer Information mittels an mehrere Stellen im Genom bindende Primer
ATE470710T1 (de) Auf nasba basiertes detektionsverfahren zur detektion des tuberkelbazillus
WO2006112939A3 (en) Compositions, methods, and kits for analyzing dna methylation

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties