ATE458044T1 - Verfahren zur nukleinsäureamplifikation - Google Patents
Verfahren zur nukleinsäureamplifikationInfo
- Publication number
- ATE458044T1 ATE458044T1 AT04820173T AT04820173T ATE458044T1 AT E458044 T1 ATE458044 T1 AT E458044T1 AT 04820173 T AT04820173 T AT 04820173T AT 04820173 T AT04820173 T AT 04820173T AT E458044 T1 ATE458044 T1 AT E458044T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- sequences
- acid amplification
- reaction
- ladder
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 230000036632 reaction speed Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/207—Modifications characterised by siRNA, miRNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/119—Strand displacement amplification [SDA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003412326 | 2003-12-10 | ||
| PCT/JP2004/018137 WO2005056790A1 (ja) | 2003-12-10 | 2004-12-06 | 核酸の増幅方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE458044T1 true ATE458044T1 (de) | 2010-03-15 |
Family
ID=34675023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04820173T ATE458044T1 (de) | 2003-12-10 | 2004-12-06 | Verfahren zur nukleinsäureamplifikation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20070298415A1 (de) |
| EP (1) | EP1715037B1 (de) |
| JP (1) | JP4249186B2 (de) |
| KR (1) | KR100968901B1 (de) |
| CN (1) | CN1890368A (de) |
| AT (1) | ATE458044T1 (de) |
| DE (1) | DE602004025624D1 (de) |
| TW (1) | TW200532025A (de) |
| WO (1) | WO2005056790A1 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004111266A1 (en) | 2003-06-17 | 2004-12-23 | Human Genetic Signatures Pty Ltd | Methods for genome amplification |
| CA2537810C (en) | 2003-09-04 | 2012-12-18 | Human Genetic Signatures Pty Ltd | Nucleic acid detection assay |
| US8168777B2 (en) | 2004-04-29 | 2012-05-01 | Human Genetic Signatures Pty. Ltd. | Bisulphite reagent treatment of nucleic acid |
| WO2006026828A1 (en) | 2004-09-10 | 2006-03-16 | Human Genetic Signatures Pty Ltd | Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn) |
| CN101111606B (zh) | 2004-12-03 | 2012-05-16 | 人类遗传标记控股有限公司 | 通过化学修饰胞嘧啶而简化微生物核酸的方法 |
| EP1883707B1 (de) * | 2005-05-26 | 2014-04-09 | Human Genetic Signatures PTY Ltd | Isothermische strangverdrängungsamplifikation unter verwendung von eine nichtreguläre base enthaltenden primern |
| GB0517005D0 (en) * | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| CA2621932A1 (en) | 2005-09-14 | 2007-03-22 | Human Genetic Signatures Pty Ltd | Assay for hpv and methylated genomic dna targets to screen for cervical cancer |
| US8143006B2 (en) | 2007-08-03 | 2012-03-27 | Igor Kutyavin | Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking |
| US8420323B2 (en) * | 2007-11-14 | 2013-04-16 | Fujifilm Corporation | Nucleic acid amplification method |
| CA2706740C (en) | 2007-11-27 | 2017-01-17 | Human Genetic Signatures Pty Ltd | Enzymes for amplification and copying bisulphite modified nucleic acids |
| EP3150727B1 (de) * | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| CN104531837A (zh) * | 2008-12-22 | 2015-04-22 | 赛卢拉有限公司 | 检测等位基因、基因组和转录物组的方法和基因型分析谱 |
| JP5616584B2 (ja) * | 2009-01-09 | 2014-10-29 | 富士フイルム株式会社 | 核酸配列の複製方法 |
| KR101912488B1 (ko) | 2011-09-07 | 2018-10-26 | 휴먼 제네틱 시그너처스 피티와이 엘티디 | 분자 검출 분석법 |
| US10370730B2 (en) | 2013-12-11 | 2019-08-06 | Saitama Medical University | Detection method for mutation in 93rd amino acid of hepatitis C virus NS5A protein, and detection kit for mutation in 93rd amino acid of hepatitis C virus NS54 protein |
| JP6346242B2 (ja) * | 2016-11-04 | 2018-06-20 | 株式会社東芝 | 核酸検出方法及びアッセイキット |
| JP6862502B2 (ja) * | 2019-07-16 | 2021-04-21 | 株式会社東芝 | 核酸検出方法及びアッセイキット |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| CA2185239C (en) | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
| US5916777A (en) * | 1995-06-07 | 1999-06-29 | Gen-Probe Incorporated | Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers |
| FR2737223B1 (fr) | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| DK0862656T3 (da) | 1995-11-21 | 2001-04-09 | Univ Yale | Unimolekylær segmentamplifikation og -detektering |
| GB9717061D0 (en) | 1997-08-13 | 1997-10-15 | Tepnel Medical Ltd | Amplification of nucleic acids |
| GB9806253D0 (en) | 1998-03-25 | 1998-05-20 | Tepnel Medical Ltd | Amplification of nucleic acids |
| EP1020534B2 (de) | 1998-11-09 | 2011-01-05 | Eiken Kagaku Kabushiki Kaisha | Verfahren zur synthese von nukleinsäuren |
| ATE362532T1 (de) | 1999-03-19 | 2007-06-15 | Takara Bio Inc | Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers |
| AU7878301A (en) * | 2000-08-23 | 2002-03-04 | Takara Shuzo Co | Method of amplifying nucleic acid |
| JP2005052002A (ja) * | 2001-07-06 | 2005-03-03 | Wakunaga Pharmaceut Co Ltd | 非環状核酸断片の増幅方法 |
| JP2003052380A (ja) * | 2001-08-20 | 2003-02-25 | Takara Bio Inc | 核酸の増幅方法 |
-
2004
- 2004-12-06 CN CNA2004800363137A patent/CN1890368A/zh active Pending
- 2004-12-06 KR KR1020067013095A patent/KR100968901B1/ko not_active Expired - Fee Related
- 2004-12-06 EP EP04820173A patent/EP1715037B1/de not_active Expired - Lifetime
- 2004-12-06 AT AT04820173T patent/ATE458044T1/de not_active IP Right Cessation
- 2004-12-06 WO PCT/JP2004/018137 patent/WO2005056790A1/ja not_active Ceased
- 2004-12-06 JP JP2005516101A patent/JP4249186B2/ja not_active Expired - Fee Related
- 2004-12-06 DE DE602004025624T patent/DE602004025624D1/de not_active Expired - Lifetime
- 2004-12-06 US US10/582,345 patent/US20070298415A1/en not_active Abandoned
- 2004-12-10 TW TW093138482A patent/TW200532025A/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE602004025624D1 (de) | 2010-04-01 |
| JPWO2005056790A1 (ja) | 2007-12-06 |
| EP1715037B1 (de) | 2010-02-17 |
| CN1890368A (zh) | 2007-01-03 |
| US20070298415A1 (en) | 2007-12-27 |
| WO2005056790A1 (ja) | 2005-06-23 |
| KR100968901B1 (ko) | 2010-07-14 |
| EP1715037A1 (de) | 2006-10-25 |
| KR20060116844A (ko) | 2006-11-15 |
| TW200532025A (en) | 2005-10-01 |
| EP1715037A4 (de) | 2007-06-20 |
| JP4249186B2 (ja) | 2009-04-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |