DE60034396D1 - Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure - Google Patents

Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure

Info

Publication number
DE60034396D1
DE60034396D1 DE60034396T DE60034396T DE60034396D1 DE 60034396 D1 DE60034396 D1 DE 60034396D1 DE 60034396 T DE60034396 T DE 60034396T DE 60034396 T DE60034396 T DE 60034396T DE 60034396 D1 DE60034396 D1 DE 60034396D1
Authority
DE
Germany
Prior art keywords
nucleic acids
nucleic acid
homopolymeric
pcr
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60034396T
Other languages
English (en)
Other versions
DE60034396T2 (de
Inventor
Andreas Missel
Dirk Loeffert
Jie Kang
Christian Korfhage
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Application granted granted Critical
Publication of DE60034396D1 publication Critical patent/DE60034396D1/de
Publication of DE60034396T2 publication Critical patent/DE60034396T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60034396T 1999-05-03 2000-05-02 Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure Expired - Lifetime DE60034396T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US304452 1999-05-03
US09/304,452 US6300069B1 (en) 1999-05-03 1999-05-03 Generation and amplification of nucleic acids from ribonucleic acids

Publications (2)

Publication Number Publication Date
DE60034396D1 true DE60034396D1 (de) 2007-05-31
DE60034396T2 DE60034396T2 (de) 2008-01-10

Family

ID=23176577

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60034396T Expired - Lifetime DE60034396T2 (de) 1999-05-03 2000-05-02 Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure

Country Status (5)

Country Link
US (1) US6300069B1 (de)
EP (1) EP1050587B1 (de)
JP (1) JP4777497B2 (de)
AT (1) ATE360093T1 (de)
DE (1) DE60034396T2 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070161010A1 (en) * 1998-08-04 2007-07-12 Gjerde Douglas T Methods and compositions for mutation analysis
US20030175723A1 (en) * 1998-08-04 2003-09-18 Gjerde Douglas T. Methods and compositions for mutation analysis
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
DK2143803T3 (en) * 2002-09-03 2016-08-01 Quanta Biosciences Inc Improved compositions and methods for cDNA synthesis
CN1316036C (zh) * 2003-01-06 2007-05-16 徐定邦 一种以聚合酶链式反应为基础的基因定量方法
US20040259115A1 (en) * 2003-01-29 2004-12-23 Schuster David M. Methods and compositions for RNA detection and quantitation
WO2004104196A1 (ja) * 2003-05-20 2004-12-02 G & G Science Co., Ltd. 緩衝剤組成物
JPWO2004104196A1 (ja) * 2003-05-20 2006-07-20 Ｇ＆Ｇサイエンス株式会社 緩衝剤組成物
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
JP2007512020A (ja) * 2003-11-26 2007-05-17 エッペンドルフ アクチェンゲゼルシャフト 染色体外核酸のインビトロ増幅のための方法及び組成物
US7824857B2 (en) 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
US7981616B2 (en) 2004-02-27 2011-07-19 Musc Foundation For Research Development Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription
US8158772B2 (en) * 2004-04-16 2012-04-17 National Institute Of Agrobiological Sciences Oligonucleotide sequences that identify species of animal
EP2287329A3 (de) 2004-07-09 2011-08-03 University of Pittsburgh of the Commonwealth System of Higher Education Identifizierung von Markern für Speiseröhrekrebs
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
DE102005047617A1 (de) * 2005-10-05 2007-04-19 Qiagen Gmbh Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US20090047673A1 (en) 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US8153401B2 (en) * 2007-05-17 2012-04-10 Applied Biosystems, Llc Method for direct amplification from crude nucleic acid samples
EP2191008B1 (de) * 2007-08-13 2013-01-09 Qiagen GmbH Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion
JP5702148B2 (ja) 2007-11-27 2015-04-15 バイオ−ラッド ラボラトリーズ，インコーポレイティド １ステップｒｔ−ｐｃｒの阻害低減
CN102084238B (zh) 2008-05-05 2016-06-01 洛斯阿拉莫斯国家安全有限责任公司 基于高度简化的侧向流动的核酸样品制备和被动流体流动控制
EP2905344B1 (de) * 2008-06-30 2016-11-09 Life Technologies Corporation Verfahren zur direkten Amplifikation aus rohen Nukleinsäureproben
WO2010062317A1 (en) 2008-10-27 2010-06-03 Infraegis, Inc. Compositions and methods for detecting food-borne pathogens
KR101881596B1 (ko) 2008-12-02 2018-07-24 웨이브 라이프 사이언시스 재팬 인코포레이티드 인 원자 변형된 핵산의 합성 방법
SG10201403841QA (en) 2009-07-06 2014-09-26 Ontorii Inc Novel nucleic acid prodrugs and methods of use thereof
EP2620428B1 (de) 2010-09-24 2019-05-22 Wave Life Sciences Ltd. Asymmetrische hilfsgruppe
CA2853615C (en) 2011-04-20 2016-07-05 Mesa Tech International, Inc. Integrated device for nucleic acid detection and identification
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
JP6128529B2 (ja) 2011-07-19 2017-05-17 ウェイブ ライフ サイエンシズ リミテッドＷａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｌｔｄ． 官能化核酸の合成のための方法
EP2559764A1 (de) 2011-08-17 2013-02-20 Qiagen GmbH Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer
EP4219516A3 (de) 2012-07-13 2024-01-10 Wave Life Sciences Ltd. Chirale steuerung
US9598458B2 (en) 2012-07-13 2017-03-21 Wave Life Sciences Japan, Inc. Asymmetric auxiliary group
BR112015000723A2 (pt) 2012-07-13 2017-06-27 Shin Nippon Biomedical Laboratories Ltd adjuvante de ácido nucléico quiral
EP3539944A1 (de) 2013-10-25 2019-09-18 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
WO2015108048A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
EP3095461A4 (de) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chirales nukleinsäure-adjuvans mit immunitätsinduktionswirkung und immunitätsinduktionsaktivator
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
BR112016016400A2 (pt) 2014-01-16 2017-10-03 Wave Life Sciences Ltd Composições de oligonucleotídeos quiralmente controlados, seu uso, sua composição farmacêutica, e métodos
WO2016106113A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106111A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106129A2 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
EP4022083A1 (de) * 2019-08-30 2022-07-06 Life Technologies Corporation Zusammensetzungen und verfahren für multiplex-pcr und genetischen analyse
JPWO2021177041A1 (de) * 2020-03-02 2021-09-10
WO2021242740A2 (en) 2020-05-26 2021-12-02 Qiagen Beverly Llc Polymerase enzyme
JP7188645B2 (ja) * 2020-07-29 2022-12-13 東洋紡株式会社 非特異的な核酸増幅を抑制する方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0787807B1 (de) * 1991-08-27 2003-05-28 F. Hoffmann-La Roche Ag Primers und Sondern zum Nachweis von Hepatitis C
EP0818461B1 (de) * 1996-07-12 2005-09-28 Toyo Boseki Kabushiki Kaisha Verfahren zur Isolierung von Ribonucleinsäuren.
WO1998037187A1 (fr) * 1997-02-21 1998-08-27 Takara Shuzo Co., Ltd. Genes associes au cancer
ES2355739T3 (es) 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).

Also Published As

Publication number Publication date
DE60034396T2 (de) 2008-01-10
EP1050587B1 (de) 2007-04-18
EP1050587A3 (de) 2004-02-04
EP1050587A2 (de) 2000-11-08
ATE360093T1 (de) 2007-05-15
JP2000342287A (ja) 2000-12-12
US6300069B1 (en) 2001-10-09
JP4777497B2 (ja) 2011-09-21

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