ATE159764T1 - Verfahren zur beherrschung der verunreinigung von nukleinsäure-amplifizierungsreaktionen - Google Patents

Verfahren zur beherrschung der verunreinigung von nukleinsäure-amplifizierungsreaktionen

Info

Publication number
ATE159764T1
ATE159764T1 AT92306396T AT92306396T ATE159764T1 AT E159764 T1 ATE159764 T1 AT E159764T1 AT 92306396 T AT92306396 T AT 92306396T AT 92306396 T AT92306396 T AT 92306396T AT E159764 T1 ATE159764 T1 AT E159764T1
Authority
AT
Austria
Prior art keywords
exo
sample
nucleic acid
acid amplification
incorporated
Prior art date
Application number
AT92306396T
Other languages
English (en)
Inventor
James L Hartley
Mark Berninger
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of ATE159764T1 publication Critical patent/ATE159764T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT92306396T 1991-07-12 1992-07-13 Verfahren zur beherrschung der verunreinigung von nukleinsäure-amplifizierungsreaktionen ATE159764T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72887491A 1991-07-12 1991-07-12

Publications (1)

Publication Number Publication Date
ATE159764T1 true ATE159764T1 (de) 1997-11-15

Family

ID=24928620

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92306396T ATE159764T1 (de) 1991-07-12 1992-07-13 Verfahren zur beherrschung der verunreinigung von nukleinsäure-amplifizierungsreaktionen

Country Status (8)

Country Link
EP (1) EP0522884B1 (de)
JP (1) JPH0811070B2 (de)
AT (1) ATE159764T1 (de)
CA (1) CA2073298C (de)
DE (1) DE69222897T2 (de)
DK (1) DK0522884T3 (de)
ES (1) ES2109983T3 (de)
GR (1) GR3025964T3 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
PL314522A1 (en) * 1993-11-23 1996-09-16 Ciba Corning Diagnostics Corp Application of antisense oligomers in a contamination controlling process during nucleic acids amplification reactions
JP3517954B2 (ja) * 1994-06-13 2004-04-12 株式会社デンソー 車両のアンチスキッド制御装置
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
CN107002145B (zh) 2014-10-08 2021-07-30 康奈尔大学 一种用于鉴定样品中的含有靶核苷酸序列的一个或多个核酸分子的方法
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
CN113293200B (zh) * 2021-05-28 2022-03-04 北京金匙基因科技有限公司 一种降低或消除二代测序中扩增产物污染的方法及应用
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
DK0415755T3 (da) * 1989-09-01 1996-01-22 Life Technologies Inc Fremgangsmåde til bekæmpelse af kontaminering af oligonukleotidafhængige nukleinsyreamplifikationsreaktioner

Also Published As

Publication number Publication date
EP0522884B1 (de) 1997-10-29
EP0522884A1 (de) 1993-01-13
DK0522884T3 (da) 1998-07-20
DE69222897D1 (de) 1997-12-04
JPH0690755A (ja) 1994-04-05
ES2109983T3 (es) 1998-02-01
JPH0811070B2 (ja) 1996-02-07
CA2073298C (en) 2007-04-17
CA2073298A1 (en) 1993-01-13
DE69222897T2 (de) 1998-10-01
GR3025964T3 (en) 1998-04-30

Similar Documents

Publication Publication Date Title
US6849404B2 (en) Polymerase chain reaction of DNA of which base sequence is completely unidentified
KR920014937A (ko) 사슬 치환에 의한 증폭방법
DE69636080D1 (de) Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
ATE106948T1 (de) Verfahren zur amplifizierung von nukleinsäuren.
DE3650679T2 (de) Oligonukleotide zur Amplifizierung von Nukleinsäuresequenzen und zur Anknüpfung einer Promotersequenz
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
NO895090L (no) Fremgangsmaate og system for deteksjonsdiagnostisering.
ATE159764T1 (de) Verfahren zur beherrschung der verunreinigung von nukleinsäure-amplifizierungsreaktionen
ES2080807T3 (es) Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.
DE59609841D1 (de) Verfahren zur Gattungs- und speziesspezifische Identifizierung von Legionellen
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
KR920014935A (ko) 사슬 치환에 의한 증폭방법

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner