ES2080807T3 - Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos. - Google Patents

Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.

Info

Publication number
ES2080807T3
ES2080807T3 ES90309492T ES90309492T ES2080807T3 ES 2080807 T3 ES2080807 T3 ES 2080807T3 ES 90309492 T ES90309492 T ES 90309492T ES 90309492 T ES90309492 T ES 90309492T ES 2080807 T3 ES2080807 T3 ES 2080807T3
Authority
ES
Spain
Prior art keywords
sample
oligonucleotide
nucleic acid
procedure
amplified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90309492T
Other languages
English (en)
Inventor
Mark Berninger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of ES2080807T3 publication Critical patent/ES2080807T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EN EL PROCESO DE ACUERDO A LA INVENCION, SE REALIZA UN PROCEDIMIENTO DE AMPLIFICACION DEPENDIENTE DE OLIGONUCLEOTIDO SOBRE UNA PRIMERA MUESTRA EN LA CUAL UNO O MAS DE LOS CUATRO RIBONUCLEOTIDOS O DEOXIRIBONUCLEOTIDOS NORMALES SON REEMPLAZADOS EN EL OLIGONUCLEOTIDO CON UNA MUESTRA EXO-NUCLEOTIDA. DESPUES DE LA AMPLIFICACION, CUALQUIER PRODUCTO AMPLIFICADO QUE PUEDA CONTAMINAR UNA SEGUNDA MUESTRA SE SOMETE A UN TRATAMIENTO FISICO, QUIMICO, ENZIMATICO O BIOLOGICO, EL CUAL PRODUCE ACIDO NUCLEICO QUE CONTIENE LA MUESTRA EXO-NUCLEOTIDA, SUSTANCIALMENTE INAMPLIFICABLE EN UNA AMPLIFICACION DEPENDIENTE DE OLIGONUCLEOTIDO SI SE USA LA MISMA SECUENCIA (S) OLIGONUCLEOTIDA. LAS SECUENCIAS DE ACIDO NUCLEICO AMPLIFICADAS DERIVADAS DE LA PRIMERA MUESTRA, LAS CUALES CONTAMINAN A LA SEGUNDA MUESTRA NO SON ADICIONALMENTE AMPLIFICADAS SUSTANCIALMENTE DURANTE LA AMPLIFICACION DEPENDIENTE DE OLIGONUCLEOTIDO DE SECUENCIAS DE ACIDO NUCLEICO DE LA SEGUNDA MUESTRA.
ES90309492T 1989-09-01 1990-08-30 Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos. Expired - Lifetime ES2080807T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40184089A 1989-09-01 1989-09-01

Publications (1)

Publication Number Publication Date
ES2080807T3 true ES2080807T3 (es) 1996-02-16

Family

ID=23589437

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90309492T Expired - Lifetime ES2080807T3 (es) 1989-09-01 1990-08-30 Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.

Country Status (8)

Country Link
EP (1) EP0415755B1 (es)
JP (1) JPH0789932B2 (es)
AT (1) ATE131878T1 (es)
CY (1) CY2073B1 (es)
DE (1) DE69024286T2 (es)
DK (1) DK0415755T3 (es)
ES (1) ES2080807T3 (es)
GR (1) GR3019092T3 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5650302A (en) * 1990-05-01 1997-07-22 Amgen Inc. Method for reducing carryover contamination in an amplification procedure
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.
DK0540693T3 (da) * 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
CA2058232A1 (en) * 1991-01-22 1992-07-23 Joseph A. Walder Process to prevent contamination by amplified nucleic acid sequence
CA2073298C (en) * 1991-07-12 2007-04-17 James L. Hartley Process for controlling contamination of nucleic acid amplification reactions
DE69332852T2 (de) * 1992-09-11 2003-12-04 F. Hoffmann-La Roche Ag, Basel Nachweis von Nukleinsäuren im Blut
US5464744A (en) * 1992-09-24 1995-11-07 Norval B. Galloway Methods and compositions for reducing false positive signals in an RNA amplification system
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
WO1995006753A1 (en) * 1993-09-03 1995-03-09 The United States of America, represented by The Secretary, Department of Health & Human Services Method and compositions for primer specific solid-phase detection of pcr products
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6818404B2 (en) 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
AU753732B2 (en) * 1997-10-23 2002-10-24 Genzyme Corporation Methods for detecting contamination in molecular diagnostics using PCR
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
EP1689879A4 (en) 2003-11-12 2008-08-27 Geneohm Sciences Inc METHODS OF HYBRIDIZING NUCLEIC ACID
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
RU2307167C2 (ru) * 2005-02-21 2007-09-27 Рамиль Ришадович Вафин Способ защиты от-пцр от контаминации продуктами амплификации на основе разрушающего действия урацил-днк-гликозилазы

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4915106A (en) * 1988-02-26 1990-04-10 Puritan-Bennett Corporation Crew oxygen mask with pneumatic comfort adjustment
ATE173508T1 (de) * 1988-05-20 1998-12-15 Hoffmann La Roche Befestigung von sequenzspezifischen proben
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)

Also Published As

Publication number Publication date
HK1000380A1 (en) 1998-03-13
EP0415755B1 (en) 1995-12-20
CY2073B1 (en) 1998-09-11
DK0415755T3 (da) 1996-01-22
ATE131878T1 (de) 1996-01-15
JPH0789932B2 (ja) 1995-10-04
DE69024286D1 (de) 1996-02-01
GR3019092T3 (en) 1996-05-31
EP0415755A2 (en) 1991-03-06
EP0415755A3 (en) 1991-08-28
JPH0391484A (ja) 1991-04-17
DE69024286T2 (de) 1996-05-30

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