DE69024286T2 - Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen - Google Patents

Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen

Info

Publication number
DE69024286T2
DE69024286T2 DE69024286T DE69024286T DE69024286T2 DE 69024286 T2 DE69024286 T2 DE 69024286T2 DE 69024286 T DE69024286 T DE 69024286T DE 69024286 T DE69024286 T DE 69024286T DE 69024286 T2 DE69024286 T2 DE 69024286T2
Authority
DE
Germany
Prior art keywords
sample
oligonucleotide
nucleic acid
procedure
amplified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69024286T
Other languages
English (en)
Other versions
DE69024286D1 (de
Inventor
Mark Berninger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of DE69024286D1 publication Critical patent/DE69024286D1/de
Publication of DE69024286T2 publication Critical patent/DE69024286T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69024286T 1989-09-01 1990-08-30 Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen Expired - Lifetime DE69024286T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40184089A 1989-09-01 1989-09-01

Publications (2)

Publication Number Publication Date
DE69024286D1 DE69024286D1 (de) 1996-02-01
DE69024286T2 true DE69024286T2 (de) 1996-05-30

Family

ID=23589437

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69024286T Expired - Lifetime DE69024286T2 (de) 1989-09-01 1990-08-30 Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen

Country Status (9)

Country Link
EP (1) EP0415755B1 (de)
JP (1) JPH0789932B2 (de)
AT (1) ATE131878T1 (de)
CY (1) CY2073B1 (de)
DE (1) DE69024286T2 (de)
DK (1) DK0415755T3 (de)
ES (1) ES2080807T3 (de)
GR (1) GR3019092T3 (de)
HK (1) HK1000380A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683896A (en) * 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5650302A (en) * 1990-05-01 1997-07-22 Amgen Inc. Method for reducing carryover contamination in an amplification procedure
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.
DK0540693T3 (da) * 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
CA2058232A1 (en) * 1991-01-22 1992-07-23 Joseph A. Walder Process to prevent contamination by amplified nucleic acid sequence
CA2073298C (en) * 1991-07-12 2007-04-17 James L. Hartley Process for controlling contamination of nucleic acid amplification reactions
EP0590327B1 (de) * 1992-09-11 2003-04-09 F. Hoffmann-La Roche Ag Nachweis von Nukleinsäuren im Blut
US5464744A (en) * 1992-09-24 1995-11-07 Norval B. Galloway Methods and compositions for reducing false positive signals in an RNA amplification system
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
AU7718594A (en) * 1993-09-03 1995-03-22 United States Of America As Represented By The Secretary Department Of Health And Human Services Method and compositions for primer specific solid-phase detection of pcr products
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6818404B2 (en) 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
JP2001520054A (ja) * 1997-10-23 2001-10-30 エグザクト サイエンシーズ コーポレイション Ｐｃｒを用いる分子診断におけるコンタミネーションを検出するための方法
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US7531306B2 (en) * 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4915106A (en) * 1988-02-26 1990-04-10 Puritan-Bennett Corporation Crew oxygen mask with pneumatic comfort adjustment
AU632494B2 (en) * 1988-05-20 1993-01-07 F. Hoffmann-La Roche Ag Immobilized sequence-specific probes
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)

Also Published As

Publication number Publication date
DK0415755T3 (da) 1996-01-22
GR3019092T3 (en) 1996-05-31
CY2073B1 (en) 1998-09-11
EP0415755A2 (de) 1991-03-06
JPH0789932B2 (ja) 1995-10-04
EP0415755B1 (de) 1995-12-20
ATE131878T1 (de) 1996-01-15
JPH0391484A (ja) 1991-04-17
EP0415755A3 (en) 1991-08-28
HK1000380A1 (en) 1998-03-13
DE69024286D1 (de) 1996-02-01
ES2080807T3 (es) 1996-02-16

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: GROSSE, BOCKHORNI, SCHUMACHER, 45133 ESSEN

8328 Change in the person/name/address of the agent

Representative=s name: BOCKHORNI & KOLLEGEN, 80687 MUENCHEN

8327 Change in the person/name/address of the patent owner

Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN