BR9604814A - Método para amplificar sequências de ácido nucleico especìficas - Google Patents

Método para amplificar sequências de ácido nucleico especìficas

Info

Publication number
BR9604814A
BR9604814A BR9604814-0A BR9604814A BR9604814A BR 9604814 A BR9604814 A BR 9604814A BR 9604814 A BR9604814 A BR 9604814A BR 9604814 A BR9604814 A BR 9604814A
Authority
BR
Brazil
Prior art keywords
nucleic acid
polymorphism
acid sequences
specific nucleic
amplifying specific
Prior art date
Application number
BR9604814-0A
Other languages
English (en)
Inventor
Alison Velyian Todd
Original Assignee
Johnson & Johnson Res Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Res Pty Ltd filed Critical Johnson & Johnson Res Pty Ltd
Publication of BR9604814A publication Critical patent/BR9604814A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MéTODO PARA AMPLIFICAR SEQuêNCIAS DE áCIDO NUCLEICO ESPECìFICAS. A presente invenção proporciona um método de detectar um polimorfismo em um indivíduo. Em uma forma o método compreende as seguintes etapas: (1) obter uma amostra contendo ácido nucleico do indivíduo; (2) amplificar a amostra de ácido nucleico da etapa (1) por um processo envolvendo termociclagem e precursores, a amplificação ocorrendo na presença de uma endonuclease de restrição termoestável que retém atividade durante termociclage, os precursores sendo selecionados de forma que eles introduzam ou no ácido nucleico amplificado do ácido nucleico não incluindo o polimorfismo ou de ácido nucleico incluindo o polimorfismo uma seq³ência reconhecida pela endonuclease de restrição termoestável; e (3) analisar o produto da etapa (2) para determinar a presença ou ausência do polimorfismo.
BR9604814-0A 1995-04-13 1996-04-12 Método para amplificar sequências de ácido nucleico especìficas BR9604814A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPN2452A AUPN245295A0 (en) 1995-04-13 1995-04-13 Assay for genetic abnormalities
PCT/AU1996/000213 WO1996032500A1 (en) 1995-04-13 1996-04-12 Method for amplifying specific nucleic acid sequences

Publications (1)

Publication Number Publication Date
BR9604814A true BR9604814A (pt) 2000-05-09

Family

ID=3786805

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9604814-0A BR9604814A (pt) 1995-04-13 1996-04-12 Método para amplificar sequências de ácido nucleico especìficas

Country Status (17)

Country Link
US (1) US6261768B1 (pt)
EP (1) EP0871767B1 (pt)
JP (1) JPH11503027A (pt)
KR (1) KR19980703858A (pt)
CN (1) CN1185181A (pt)
AR (1) AR001619A1 (pt)
AT (1) ATE214429T1 (pt)
AU (1) AUPN245295A0 (pt)
BR (1) BR9604814A (pt)
CA (1) CA2218026A1 (pt)
DE (1) DE69619864T2 (pt)
DK (1) DK0871767T3 (pt)
ES (1) ES2174063T3 (pt)
IL (1) IL117884A0 (pt)
TW (1) TW546382B (pt)
WO (1) WO1996032500A1 (pt)
ZA (1) ZA962941B (pt)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL164182A0 (en) * 1998-03-05 2005-12-18 Johnson & Johnson Res Pty Ltd Zymogenic nucleic acid detection methods, and related molecules and kits
EP0953635B1 (en) 1998-04-28 2012-08-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
DE60014399T2 (de) 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
CA2424584A1 (en) * 2000-10-02 2002-04-11 Ortho-Clinical Diagnostics, Inc. Detection of ras mutations
US7052837B2 (en) * 2001-03-13 2006-05-30 The Board Of Trustees Of The University Of Arkansas Histoplasma capsulatum catalase sequences and their use in the detection of Histoplamsa capsulatum and histoplasmosis
US20030162174A1 (en) * 2001-06-11 2003-08-28 Sutherland John W. Detecting nucleic acid deletion sequences
KR20040009054A (ko) * 2002-07-22 2004-01-31 김철욱 인적 유전인자의 개체별 특성파악을 위한 유전자 검사방법
US20040054160A1 (en) * 2002-09-16 2004-03-18 Santona Pal Nucleic-acid ink compositions for arraying onto a solid support
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004106555A2 (en) * 2003-05-22 2004-12-09 Dow Agrosciences Llc High-throughput methods of screening dna for deletions and other mutations
DK2444505T3 (da) * 2004-07-23 2017-01-02 Pacific Edge Ltd Urinmarkører til påvisning af blærecancer
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US8652780B2 (en) 2007-03-26 2014-02-18 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8999634B2 (en) 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
CN101280338B (zh) * 2007-12-13 2011-08-17 中国人民解放军第三军医大学第一附属医院 一种检测核酸多态性的核酸扩增方法
CA2718137A1 (en) * 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
WO2010107946A2 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
WO2013003630A2 (en) * 2011-06-28 2013-01-03 Igor Kutyavin Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
EP2975124B1 (en) * 2013-03-14 2019-03-06 Takara Bio Inc. Method for using heat-resistant mismatch endonuclease
WO2016039377A1 (ja) 2014-09-11 2016-03-17 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
CN107614681A (zh) * 2015-03-20 2018-01-19 宝生物工程株式会社 用于检测靶核酸的高灵敏度方法
CN114014472B (zh) * 2022-01-07 2022-04-26 山东玲珑轮胎股份有限公司 一种废旧轮胎回收中产生的废水处理方法及系统

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
ES2092527T3 (es) 1990-06-08 1996-12-01 Behringwerke Ag Metodo para la determinacion cuantitativa de secuencias de adn.
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU652214B2 (en) * 1991-01-31 1994-08-18 Becton Dickinson & Company Strand displacement amplification "Sealing Clerk - sealing is to be refunded"
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5512441A (en) 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method

Also Published As

Publication number Publication date
EP0871767A1 (en) 1998-10-21
IL117884A0 (en) 1996-08-04
ES2174063T3 (es) 2002-11-01
DK0871767T3 (da) 2002-07-08
CN1185181A (zh) 1998-06-17
ATE214429T1 (de) 2002-03-15
CA2218026A1 (en) 1996-10-17
AUPN245295A0 (en) 1995-05-11
TW546382B (en) 2003-08-11
WO1996032500A1 (en) 1996-10-17
EP0871767A4 (en) 1999-09-01
US6261768B1 (en) 2001-07-17
AR001619A1 (es) 1997-11-26
DE69619864T2 (de) 2002-11-07
MX9707885A (es) 1998-06-28
EP0871767B1 (en) 2002-03-13
ZA962941B (en) 1996-12-04
DE69619864D1 (de) 2002-04-18
KR19980703858A (ko) 1998-12-05
JPH11503027A (ja) 1999-03-23

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Legal Events

Date Code Title Description
EG Technical examination (opinion): publication of technical examination (opinion)
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements