DE69217501D1 - Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns - Google Patents

Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns

Info

Publication number
DE69217501D1
DE69217501D1 DE69217501T DE69217501T DE69217501D1 DE 69217501 D1 DE69217501 D1 DE 69217501D1 DE 69217501 T DE69217501 T DE 69217501T DE 69217501 T DE69217501 T DE 69217501T DE 69217501 D1 DE69217501 D1 DE 69217501D1
Authority
DE
Germany
Prior art keywords
interest
dna fragment
fragment
dna
standard
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69217501T
Other languages
English (en)
Other versions
DE69217501T2 (de
Inventor
Christophe Pannetier
Madeleine Cochet
Sylvie Darche
Philippe Kourilsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut Pasteur de Lille
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille, Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut Pasteur de Lille
Application granted granted Critical
Publication of DE69217501D1 publication Critical patent/DE69217501D1/de
Publication of DE69217501T2 publication Critical patent/DE69217501T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69217501T 1991-11-15 1992-11-13 Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns Expired - Fee Related DE69217501T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR919114089A FR2683827B1 (fr) 1991-11-15 1991-11-15 Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.
PCT/FR1992/001059 WO1993010257A1 (fr) 1991-11-15 1992-11-13 Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique d'adn

Publications (2)

Publication Number Publication Date
DE69217501D1 true DE69217501D1 (de) 1997-03-27
DE69217501T2 DE69217501T2 (de) 1997-09-18

Family

ID=9418974

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69217501T Expired - Fee Related DE69217501T2 (de) 1991-11-15 1992-11-13 Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns

Country Status (8)

Country Link
US (1) US5747246A (de)
EP (1) EP0613503B1 (de)
JP (1) JPH07501215A (de)
AT (1) ATE148924T1 (de)
CA (1) CA2123680A1 (de)
DE (1) DE69217501T2 (de)
FR (1) FR2683827B1 (de)
WO (1) WO1993010257A1 (de)

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US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
EP0929696A2 (de) * 1996-09-09 1999-07-21 Eppendorf Array Technologies (E.A.T.) Verfahren und reagentiensatz zur erkennung und/oder quantifizierung von nukleinsäuren durch sandwich-hybridisierung an der festphase
BE1010608A3 (fr) * 1996-09-09 1998-11-03 Jose Remacle Procede de quantification d'une sequence d'acides nucleiques.
FR2765590B1 (fr) * 1997-07-01 2002-08-09 Pasteur Institut Methode de semi-quantification d'alleles
DE19934084A1 (de) * 1999-07-15 2001-01-18 Universitaetsklinikum Charite Verfahren und Testbesteck zur Markierung von DNA-Fragmenten während einer PCR-Reaktion
GB9929381D0 (en) * 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7718354B2 (en) 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) * 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) * 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
JP4683922B2 (ja) * 2002-09-06 2011-05-18 トラスティーズ オブ ボストン ユニバーシティ 遺伝子発現の定量方法
EP1578399A4 (de) 2002-12-06 2007-11-28 Isis Pharmaceuticals Inc Verfahren für die rasche identifikation von pathogenen bei tier und mensch
US8046171B2 (en) * 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20060240412A1 (en) * 2003-09-11 2006-10-26 Hall Thomas A Compositions for use in identification of adenoviruses
US20100035239A1 (en) * 2003-09-11 2010-02-11 Isis Pharmaceuticals, Inc. Compositions for use in identification of bacteria
US7666592B2 (en) * 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
EP2458619B1 (de) 2004-05-24 2017-08-02 Ibis Biosciences, Inc. Massenspektrometrie mit selektivem Ionfilter durch digitale Schwelle
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
US8084207B2 (en) * 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
AU2006272776B2 (en) 2005-07-21 2012-01-19 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
US8871471B2 (en) 2007-02-23 2014-10-28 Ibis Biosciences, Inc. Methods for rapid forensic DNA analysis
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
WO2010093943A1 (en) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ionization probe assemblies
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
WO2011008971A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Lift and mount apparatus
US20110091882A1 (en) * 2009-10-02 2011-04-21 Ibis Biosciences, Inc. Determination of methylation status of polynucleotides
ES2628739T3 (es) * 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
EP2877952B1 (de) 2012-07-27 2023-04-12 Gen-Probe Incorporated Dual-referenzkalibrierverfahren und system zum quantifizieren von polynukleotiden

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US5082935A (en) * 1988-12-15 1992-01-21 Amoco Corporation Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
ES2092527T3 (es) * 1990-06-08 1996-12-01 Behringwerke Ag Metodo para la determinacion cuantitativa de secuencias de adn.
GB9016163D0 (en) * 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method

Also Published As

Publication number Publication date
EP0613503A1 (de) 1994-09-07
FR2683827A1 (fr) 1993-05-21
FR2683827B1 (fr) 1994-03-04
CA2123680A1 (fr) 1993-05-27
EP0613503B1 (de) 1997-02-12
ATE148924T1 (de) 1997-02-15
JPH07501215A (ja) 1995-02-09
DE69217501T2 (de) 1997-09-18
US5747246A (en) 1998-05-05
WO1993010257A1 (fr) 1993-05-27

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