MX9707885A - Metodo para amplificar secuencias especificas de acido nucleico. - Google Patents
Metodo para amplificar secuencias especificas de acido nucleico.Info
- Publication number
- MX9707885A MX9707885A MX9707885A MX9707885A MX9707885A MX 9707885 A MX9707885 A MX 9707885A MX 9707885 A MX9707885 A MX 9707885A MX 9707885 A MX9707885 A MX 9707885A MX 9707885 A MX9707885 A MX 9707885A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- polymorphism
- acid sequences
- specific nucleic
- amplifying specific
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invencion proporciona un método para detectar un polimorfismo genético en un individuo. En una forma el método comprenden las siguientes etapas: (1) obtener una muestra que contiene ácido nucleico del individuo; (2) la muestra del ácido nucleico de la etapa (1) por un proceso que implica termociclado e iniciadores, la amplificacion ocurre en la presencia de endonucleasa de restriccion termoestable, la cual retiene su actividad durante el termociclado, los iniciadores que son seleccionados de tal forma que ellos inducen ya sea en el ácido nucleico amplificado del ácido nucleico que no incluye el polimorfismo o del ácido nucleico que incluye el polimorfismo, una secuencia reconocida por la endonucleasa de restriccion termoestable; y (3) analizar el producto de la etapa (2) para determinar la presencia o ausencia del polimorfismo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PNPN2452 | 1995-04-13 | ||
AUPN2452A AUPN245295A0 (en) | 1995-04-13 | 1995-04-13 | Assay for genetic abnormalities |
Publications (2)
Publication Number | Publication Date |
---|---|
MX9707885A true MX9707885A (es) | 1998-06-28 |
MXPA97007885A MXPA97007885A (es) | 1998-10-30 |
Family
ID=
Also Published As
Publication number | Publication date |
---|---|
CA2218026A1 (en) | 1996-10-17 |
IL117884A0 (en) | 1996-08-04 |
AR001619A1 (es) | 1997-11-26 |
KR19980703858A (ko) | 1998-12-05 |
DK0871767T3 (da) | 2002-07-08 |
ATE214429T1 (de) | 2002-03-15 |
WO1996032500A1 (en) | 1996-10-17 |
EP0871767A4 (en) | 1999-09-01 |
JPH11503027A (ja) | 1999-03-23 |
US6261768B1 (en) | 2001-07-17 |
BR9604814A (pt) | 2000-05-09 |
ZA962941B (en) | 1996-12-04 |
TW546382B (en) | 2003-08-11 |
ES2174063T3 (es) | 2002-11-01 |
EP0871767B1 (en) | 2002-03-13 |
DE69619864T2 (de) | 2002-11-07 |
AUPN245295A0 (en) | 1995-05-11 |
DE69619864D1 (de) | 2002-04-18 |
EP0871767A1 (en) | 1998-10-21 |
CN1185181A (zh) | 1998-06-17 |
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