WO2002079512A3 - Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr - Google Patents

Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr Download PDF

Info

Publication number
WO2002079512A3
WO2002079512A3 PCT/US2002/006324 US0206324W WO02079512A3 WO 2002079512 A3 WO2002079512 A3 WO 2002079512A3 US 0206324 W US0206324 W US 0206324W WO 02079512 A3 WO02079512 A3 WO 02079512A3
Authority
WO
WIPO (PCT)
Prior art keywords
aspergillus fumigatus
fungus aspergillus
quantification
detection
quantitative pcr
Prior art date
Application number
PCT/US2002/006324
Other languages
French (fr)
Other versions
WO2002079512A2 (en
Inventor
Patricia Cruz-Perez
Mark P Buttner
Original Assignee
Univ Nevada Las Vegas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nevada Las Vegas filed Critical Univ Nevada Las Vegas
Priority to AU2002306628A priority Critical patent/AU2002306628A1/en
Publication of WO2002079512A2 publication Critical patent/WO2002079512A2/en
Publication of WO2002079512A3 publication Critical patent/WO2002079512A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

A method for detecting the fungus Aspergillus fumigatus includes isolating DNA from a sample suspected of containing the fungus Aspergillus fumigatus. The method further includes subjecting the DNA to polymerase chain reaction amplification utilizing at least one primer. The at least one primer includes one of a 5'CGCGTCCGGTCCTCG3' and 5'TTAGAAAAATAAAGTTGGGTGTCGG3' base sequence. The method also includes detecting the fungus Aspergillus fumigatus by visualizing the product of the polymerase chain reaction.
PCT/US2002/006324 2001-03-29 2002-02-28 Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr WO2002079512A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002306628A AU2002306628A1 (en) 2001-03-29 2002-02-28 Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28071301P 2001-03-29 2001-03-29
US60/280,713 2001-03-29

Publications (2)

Publication Number Publication Date
WO2002079512A2 WO2002079512A2 (en) 2002-10-10
WO2002079512A3 true WO2002079512A3 (en) 2004-03-11

Family

ID=23074290

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/006324 WO2002079512A2 (en) 2001-03-29 2002-02-28 Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr

Country Status (2)

Country Link
AU (1) AU2002306628A1 (en)
WO (1) WO2002079512A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0621864D0 (en) * 2006-11-02 2006-12-13 Univ Manchester Assay for fungal infection
CZ302670B6 (en) * 2009-04-21 2011-08-24 Masarykova Univerzita Method of diagnosing invasive aspergillosis and oligonucleotides used in this method
PT107037B (en) * 2013-07-03 2017-09-13 Chromoperformance S A NUCLEIC PEPTIDE ACID (PNA) PROBE, CARTON AND METHOD FOR DETERMINATION OF ASPERGILLUS FUMIGATUS AND RESTRICTIVE APPLICATIONS
CN107130015B (en) * 2016-02-29 2023-07-11 上海翔琼生物技术有限公司 Fluorescent quantitative PCR kit for detecting aspergillus fumigatus
CN107164478A (en) * 2017-05-27 2017-09-15 山西维尔生物乳制品有限公司 A kind of probe, primer and genetic chip for differentiating aspergillus fumigatus
CN108070675B (en) * 2018-02-10 2020-04-21 杭州缔蓝生物技术有限公司 Primer probe combination and fluorescent quantitative PCR kit for simultaneously detecting three kinds of aspergillus

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
WO2000073499A2 (en) * 1999-05-28 2000-12-07 Innogenetics N.V. Nucleic acid probes and methods for detecting clinically important fungal pathogens
FR2796079A1 (en) * 1999-07-07 2001-01-12 Foad Chaib Nucleotide sequences from mitochondrial DNA of fungi, useful as probes and targets, e.g. for diagnostic detection and identification in medicine or agriculture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
WO2000073499A2 (en) * 1999-05-28 2000-12-07 Innogenetics N.V. Nucleic acid probes and methods for detecting clinically important fungal pathogens
FR2796079A1 (en) * 1999-07-07 2001-01-12 Foad Chaib Nucleotide sequences from mitochondrial DNA of fungi, useful as probes and targets, e.g. for diagnostic detection and identification in medicine or agriculture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MONDONP ET AL: "MOLECULAR TYPING OF ASPERGILLUS FUMIGATUS STRAIN BY SEQUENCE-SPECIFIC DNA PRIMER (SSDP) ANALYSIS", FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, ELSEVIER SCIENCE B.V., AMSTERDAM, NL, vol. 17, 1997, pages 95 - 102, XP000960740, ISSN: 0928-8244 *

Also Published As

Publication number Publication date
AU2002306628A1 (en) 2002-10-15
WO2002079512A2 (en) 2002-10-10

Similar Documents

Publication Publication Date Title
EP0875584A3 (en) Nucleic acid assays
WO2001083823A8 (en) Methods and compositions for polynucleotide analysis using generic capture sequences
WO2003060163A8 (en) Discrimination and detection of target nucleotide sequences using mass spectrometry
HK1068651A1 (en) Detection system
WO2000049182A3 (en) Analytical methods and materials for nucleic acid detection
WO1996036736A3 (en) Wide dynamic range nucleic acid detection using an aggregate primer series
WO1999060159A3 (en) Method for using unequal primer concentrations for generating nucleic acid amplification products
WO2004106547A3 (en) Fast method for detecting micro-organisms in food samples
WO1998041650A3 (en) Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
IL147845A0 (en) Method for relative quantization of methylation of cytosin-type bases in dna samples
DE60016275D1 (en) Helicobacter pylori antigene in blut
NZ322938A (en) Method of detecting telomerase activity
AU2003289927A1 (en) Multiplex assay detection of pathogenic organisms
WO1997032040A3 (en) Nucleic acid sequence detection
WO2002068473A8 (en) Biosensor for detecting chemical agents
WO2002070739A3 (en) Assay for identifying inhibitors of the rna dependent rna polymerase (ns5b) of hcv
WO2001068913A3 (en) Nucleic acid detection method and system
WO2002079512A3 (en) Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr
WO2001040504A3 (en) Methods and compositions for detecting nitroaromatic compounds
SG153624A1 (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid
WO2004050911A3 (en) Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
WO2004046344A3 (en) Polynucleotide sequence detection assays
WO2000000638A3 (en) Tagging of rna amplicons generated by transcription-based amplification
AU2001289754A1 (en) Method and device for analysing chemical or biological samples
WO2001061038A3 (en) Method for detecting a biological entity in a sample

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP