WO2001068913A3 - Nucleic acid detection method and system - Google Patents

Nucleic acid detection method and system Download PDF

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Publication number
WO2001068913A3
WO2001068913A3 PCT/IB2001/000465 IB0100465W WO0168913A3 WO 2001068913 A3 WO2001068913 A3 WO 2001068913A3 IB 0100465 W IB0100465 W IB 0100465W WO 0168913 A3 WO0168913 A3 WO 0168913A3
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WO
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Patent type
Prior art keywords
nucleic acid
methods
acid detection
system
detection method
Prior art date
Application number
PCT/IB2001/000465
Other languages
French (fr)
Other versions
WO2001068913A2 (en )
Inventor
Christine Peponnet
Jan Sudor
Original Assignee
Genset Sa
Christine Peponnet
Jan Sudor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Abstract

The present invention provides novel nucleic acid detection methods based on nucleic acid template-directed primer extension assays. Also provided are kits and genetic analysis methods. Included are microsequencing methods comprising detecting an incorporated nucleotide by measuring direct fluorescence intensity, fluorescence-based nucleic acid detection methods using oligonucleotide primers hybridizing to both complementary strands of a nucleic acid sample, and methods for decreasing background signal in nucleic acid template-directed primer extension assays.
PCT/IB2001/000465 2000-03-13 2001-03-13 Nucleic acid detection method and system WO2001068913A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US18876400 true 2000-03-13 2000-03-13
US60/188,764 2000-03-13

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU4099001A AU4099001A (en) 2000-03-13 2001-03-13 Nucleic acid detection method and system

Publications (2)

Publication Number Publication Date
WO2001068913A2 true WO2001068913A2 (en) 2001-09-20
WO2001068913A3 true true WO2001068913A3 (en) 2002-10-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/000465 WO2001068913A3 (en) 2000-03-13 2001-03-13 Nucleic acid detection method and system

Country Status (1)

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WO (1) WO2001068913A3 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60217972T2 (en) * 2001-11-13 2007-11-15 Serono Genetics Institute S.A. Method to increase the efficiency of enzymatic purification processes
US7347976B2 (en) 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7192560B2 (en) 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
WO2004022721A3 (en) 2002-09-06 2004-09-16 Charles R Cantor Quantification of gene expression
DE602004021902D1 (en) 2003-01-17 2009-08-20 Univ Boston haplotype analysis
US7981600B2 (en) 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
JP2007508017A (en) 2003-10-08 2007-04-05 ザ トラスティーズ オブ ボストン ユニバーシティ Methods for prenatal diagnosis of chromosomal abnormalities
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1721014B1 (en) 2004-02-18 2013-07-17 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0969103A2 (en) * 1993-06-22 2000-01-05 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
WO2000011220A1 (en) * 1998-08-21 2000-03-02 Washington University Fluorescence polarization in nucleic acid analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0969103A2 (en) * 1993-06-22 2000-01-05 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
WO2000011220A1 (en) * 1998-08-21 2000-03-02 Washington University Fluorescence polarization in nucleic acid analysis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN AND KWOK: "TEMPLATE-DIRECTED DYE-TERMINATOR INCORPORATION (TDI) ASSAY: A HOMOGENEOUS DNA DIAGNOSTIC METHOD BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 2, January 1997 (1997-01-01), pages 347 - 353, XP002125080, ISSN: 0305-1048 *
PASTINEN T ET AL: "MINISEQUENCING: A SPECIFIC TOOL FOR DNA ANALYSIS AND DIAGNOSTICS ONOLIGONUCLEOTIDE ARRAYS", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 7, no. 6, 1 June 1997 (1997-06-01), pages 606 - 614, XP000699761, ISSN: 1088-9051 *
SYVANEN A-C ET AL: "DETECTION OF POINT MUTATIONS BY SOLID-PHASE METHODS", HUMAN MUTATION, WILEY-LISS, NEW YORK, NY, US, vol. 3, no. 3, 1994, pages 172 - 179, XP000600258, ISSN: 1059-7794 *

Also Published As

Publication number Publication date Type
WO2001068913A2 (en) 2001-09-20 application

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