ATE148924T1 - Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns - Google Patents
Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dnsInfo
- Publication number
- ATE148924T1 ATE148924T1 AT93900249T AT93900249T ATE148924T1 AT E148924 T1 ATE148924 T1 AT E148924T1 AT 93900249 T AT93900249 T AT 93900249T AT 93900249 T AT93900249 T AT 93900249T AT E148924 T1 ATE148924 T1 AT E148924T1
- Authority
- AT
- Austria
- Prior art keywords
- interest
- dna fragment
- fragment
- dna
- standard
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title abstract 19
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title 1
- 239000013615 primer Substances 0.000 abstract 4
- 239000003155 DNA primer Substances 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 238000004925 denaturation Methods 0.000 abstract 1
- 230000036425 denaturation Effects 0.000 abstract 1
- 239000012429 reaction media Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR919114089A FR2683827B1 (fr) | 1991-11-15 | 1991-11-15 | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE148924T1 true ATE148924T1 (de) | 1997-02-15 |
Family
ID=9418974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93900249T ATE148924T1 (de) | 1991-11-15 | 1992-11-13 | Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5747246A (de) |
| EP (1) | EP0613503B1 (de) |
| JP (1) | JPH07501215A (de) |
| AT (1) | ATE148924T1 (de) |
| CA (1) | CA2123680A1 (de) |
| DE (1) | DE69217501T2 (de) |
| FR (1) | FR2683827B1 (de) |
| WO (1) | WO1993010257A1 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA936015B (en) * | 1992-08-24 | 1994-03-10 | Akzo Nv | Elimination of false negatives in nuleic acid detection. |
| ATE178946T1 (de) * | 1993-07-09 | 1999-04-15 | Akzo Nobel Nv | Verbessertes verfahren für die quantifizierung von nukleinsäuren |
| US5837501A (en) * | 1993-07-09 | 1998-11-17 | Akzo Nobel N.V. | Nucleic acid quantitation by co-amplification of target with multiple internal controls |
| US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| BE1010608A3 (fr) * | 1996-09-09 | 1998-11-03 | Jose Remacle | Procede de quantification d'une sequence d'acides nucleiques. |
| EP0929696A2 (de) * | 1996-09-09 | 1999-07-21 | Eppendorf Array Technologies (E.A.T.) | Verfahren und reagentiensatz zur erkennung und/oder quantifizierung von nukleinsäuren durch sandwich-hybridisierung an der festphase |
| FR2765590B1 (fr) * | 1997-07-01 | 2002-08-09 | Pasteur Institut | Methode de semi-quantification d'alleles |
| DE19934084A1 (de) * | 1999-07-15 | 2001-01-18 | Universitaetsklinikum Charite | Verfahren und Testbesteck zur Markierung von DNA-Fragmenten während einer PCR-Reaktion |
| GB9929381D0 (en) * | 1999-12-10 | 2000-02-09 | Pyrosequencing Ab | A method of assessing the amount of nucleic acid in a sample |
| US6783934B1 (en) | 2000-05-01 | 2004-08-31 | Cepheid, Inc. | Methods for quantitative analysis of nucleic acid amplification reaction |
| WO2004060278A2 (en) | 2002-12-06 | 2004-07-22 | Isis Pharmaceuticals, Inc. | Methods for rapid identification of pathogens in humans and animals |
| US7666588B2 (en) * | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
| US7718354B2 (en) | 2001-03-02 | 2010-05-18 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
| US20030027135A1 (en) * | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
| US20040121309A1 (en) * | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues |
| US7226739B2 (en) | 2001-03-02 | 2007-06-05 | Isis Pharmaceuticals, Inc | Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations |
| US8073627B2 (en) * | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
| US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
| JP4683922B2 (ja) * | 2002-09-06 | 2011-05-18 | トラスティーズ オブ ボストン ユニバーシティ | 遺伝子発現の定量方法 |
| US8046171B2 (en) * | 2003-04-18 | 2011-10-25 | Ibis Biosciences, Inc. | Methods and apparatus for genetic evaluation |
| US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US20100035239A1 (en) * | 2003-09-11 | 2010-02-11 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of bacteria |
| US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US8288523B2 (en) | 2003-09-11 | 2012-10-16 | Ibis Biosciences, Inc. | Compositions for use in identification of bacteria |
| US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US20060240412A1 (en) * | 2003-09-11 | 2006-10-26 | Hall Thomas A | Compositions for use in identification of adenoviruses |
| US7666592B2 (en) * | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
| US8119336B2 (en) | 2004-03-03 | 2012-02-21 | Ibis Biosciences, Inc. | Compositions for use in identification of alphaviruses |
| EP2458619B1 (de) | 2004-05-24 | 2017-08-02 | Ibis Biosciences, Inc. | Massenspektrometrie mit selektivem Ionfilter durch digitale Schwelle |
| US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
| US7811753B2 (en) | 2004-07-14 | 2010-10-12 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
| WO2006135400A2 (en) | 2004-08-24 | 2006-12-21 | Isis Pharmaceuticals, Inc. | Methods for rapid identification of recombinant organisms |
| US8182992B2 (en) | 2005-03-03 | 2012-05-22 | Ibis Biosciences, Inc. | Compositions for use in identification of adventitious viruses |
| US8084207B2 (en) * | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
| EP1904655A2 (de) | 2005-07-21 | 2008-04-02 | Isis Pharmaceuticals, Inc. | Verfahren zur raschen identifizierung und quantifizierung von nukleinsäurevarianten |
| EP2064332B1 (de) | 2006-09-14 | 2012-07-18 | Ibis Biosciences, Inc. | Gezielte gesamtgenomamplifizierung zur identifizierung von krankheitserregern |
| JP5680304B2 (ja) | 2007-02-23 | 2015-03-04 | アイビス バイオサイエンシズ インコーポレイティッド | 迅速な法医学的dna分析法 |
| US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
| US9689031B2 (en) * | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| WO2010033599A2 (en) | 2008-09-16 | 2010-03-25 | Ibis Biosciences, Inc. | Mixing cartridges, mixing stations, and related kits, systems, and methods |
| WO2010033625A1 (en) | 2008-09-16 | 2010-03-25 | Ibis Biosciences, Inc. | Microplate handling systems and related computer program products and methods |
| EP2347254A2 (de) | 2008-09-16 | 2011-07-27 | Ibis Biosciences, Inc. | Probenverarbeitungseinheiten, -systeme und damit verbundene verfahren |
| EP2396803A4 (de) | 2009-02-12 | 2016-10-26 | Ibis Biosciences Inc | Ionisationssondenanordnungen |
| EP2454000A4 (de) | 2009-07-17 | 2016-08-10 | Ibis Biosciences Inc | Systeme zur identifizierung von biowirkstoffen |
| US8950604B2 (en) | 2009-07-17 | 2015-02-10 | Ibis Biosciences, Inc. | Lift and mount apparatus |
| WO2011041695A1 (en) * | 2009-10-02 | 2011-04-07 | Ibis Biosciences, Inc. | Determination of methylation status of polynucleotides |
| US9890408B2 (en) * | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| WO2014018885A2 (en) | 2012-07-27 | 2014-01-30 | Gen-Probe Incorporated | Dual reference calibration method and system for quantifying polynucleotides |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5082935A (en) * | 1988-12-15 | 1992-01-21 | Amoco Corporation | Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination |
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| DK0461496T3 (da) * | 1990-06-08 | 1997-01-06 | Behringwerke Ag | Fremgangsmåde til kvantitativ bestemmelse af DNA-sekvenser |
| GB9016163D0 (en) * | 1990-07-24 | 1990-09-05 | Cemu Bioteknik | Chemical method |
-
1991
- 1991-11-15 FR FR919114089A patent/FR2683827B1/fr not_active Expired - Fee Related
-
1992
- 1992-11-13 DE DE69217501T patent/DE69217501T2/de not_active Expired - Fee Related
- 1992-11-13 CA CA002123680A patent/CA2123680A1/fr not_active Abandoned
- 1992-11-13 JP JP5509031A patent/JPH07501215A/ja active Pending
- 1992-11-13 WO PCT/FR1992/001059 patent/WO1993010257A1/fr not_active Ceased
- 1992-11-13 AT AT93900249T patent/ATE148924T1/de not_active IP Right Cessation
- 1992-11-13 EP EP93900249A patent/EP0613503B1/de not_active Expired - Lifetime
-
1994
- 1994-06-21 US US08/263,413 patent/US5747246A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2123680A1 (fr) | 1993-05-27 |
| JPH07501215A (ja) | 1995-02-09 |
| US5747246A (en) | 1998-05-05 |
| EP0613503A1 (de) | 1994-09-07 |
| WO1993010257A1 (fr) | 1993-05-27 |
| FR2683827B1 (fr) | 1994-03-04 |
| FR2683827A1 (fr) | 1993-05-21 |
| EP0613503B1 (de) | 1997-02-12 |
| DE69217501T2 (de) | 1997-09-18 |
| DE69217501D1 (de) | 1997-03-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |