ATE178946T1 - Verbessertes verfahren für die quantifizierung von nukleinsäuren - Google Patents

Verbessertes verfahren für die quantifizierung von nukleinsäuren

Info

Publication number
ATE178946T1
ATE178946T1 AT94924750T AT94924750T ATE178946T1 AT E178946 T1 ATE178946 T1 AT E178946T1 AT 94924750 T AT94924750 T AT 94924750T AT 94924750 T AT94924750 T AT 94924750T AT E178946 T1 ATE178946 T1 AT E178946T1
Authority
AT
Austria
Prior art keywords
nucleic acid
analyte
quantification
constructs
construct
Prior art date
Application number
AT94924750T
Other languages
English (en)
Inventor
Marinus Gerardus Joh Beuningen
Tim Kievits
Thomas Augustinus Maria Beumer
Original Assignee
Akzo Nobel Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel Nv filed Critical Akzo Nobel Nv
Application granted granted Critical
Publication of ATE178946T1 publication Critical patent/ATE178946T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT94924750T 1993-07-09 1994-07-08 Verbessertes verfahren für die quantifizierung von nukleinsäuren ATE178946T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93202015 1993-07-09
EP93203424 1993-12-06

Publications (1)

Publication Number Publication Date
ATE178946T1 true ATE178946T1 (de) 1999-04-15

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94924750T ATE178946T1 (de) 1993-07-09 1994-07-08 Verbessertes verfahren für die quantifizierung von nukleinsäuren

Country Status (10)

Country Link
JP (1) JP3594598B2 (de)
KR (1) KR100312800B1 (de)
AT (1) ATE178946T1 (de)
AU (1) AU668659B2 (de)
CA (1) CA2143857C (de)
DE (1) DE69417863T2 (de)
DK (1) DK0662156T3 (de)
ES (1) ES2131699T3 (de)
FI (1) FI112094B (de)
WO (1) WO1995002067A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0722508B1 (de) * 1993-09-22 2003-04-02 Igen International, Inc. Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
JP3996639B2 (ja) * 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (fr) * 1997-07-01 2002-08-09 Pasteur Institut Methode de semi-quantification d'alleles
WO2000000638A2 (en) * 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (de) * 1998-12-22 2000-06-28 Universiteit van Amsterdam Empfindliches Testverfahren zum Nachweis oder zur Quantifizierung der Nukleinsäure des menschlichen Cytomegalovirus
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
ATE304061T1 (de) * 1999-07-23 2005-09-15 Gen Probe Inc Polynukleotid amplifizierungsverfahren
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
US7790368B1 (en) 2000-09-22 2010-09-07 Hitachi, Ltd. Method for analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Ｄｎａ定量方法、及び遺伝子解析方法
JP2011062119A (ja) 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
ES2572391T3 (es) 2011-02-25 2016-05-31 Novartis Ag Control exógeno interno positivo para la detección de virus
KR102108855B1 (ko) 2017-10-30 2020-05-12 한국표준과학연구원 안정동위원소 표지 핵산을 내부표준물질로 활용하는 핵산 정량 방법 및 이의 용도

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
ES2214472T3 (es) * 1991-08-02 2004-09-16 Biomerieux B.V. Cuantificacion de acidos nucleicos.
FR2683827B1 (fr) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.

Also Published As

Publication number Publication date
CA2143857C (en) 2005-09-20
DE69417863D1 (de) 1999-05-20
FI112094B (fi) 2003-10-31
AU7492894A (en) 1995-02-06
WO1995002067A1 (en) 1995-01-19
ES2131699T3 (es) 1999-08-01
JPH08501222A (ja) 1996-02-13
FI951082A0 (fi) 1995-03-08
CA2143857A1 (en) 1995-01-19
FI951082A (fi) 1995-03-08
KR100312800B1 (ko) 2002-02-28
DE69417863T2 (de) 1999-10-07
JP3594598B2 (ja) 2004-12-02
DK0662156T3 (da) 1999-10-25
AU668659B2 (en) 1996-05-09

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