CA2143857A1 - Improved method for the quantification of nucleic acid - Google Patents

Improved method for the quantification of nucleic acid

Info

Publication number
CA2143857A1
CA2143857A1 CA002143857A CA2143857A CA2143857A1 CA 2143857 A1 CA2143857 A1 CA 2143857A1 CA 002143857 A CA002143857 A CA 002143857A CA 2143857 A CA2143857 A CA 2143857A CA 2143857 A1 CA2143857 A1 CA 2143857A1
Authority
CA
Canada
Prior art keywords
nucleic acid
analyte
quantification
constructs
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002143857A
Other languages
French (fr)
Other versions
CA2143857C (en
Inventor
Marinus G. J. Van Beuningen
Tim Kievits
Thomas A. M. Beumer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux BV
Original Assignee
Marinus G. J. Van Beuningen
Tim Kievits
Thomas A. M. Beumer
Akzo Nobel Nv
Biomerieux B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marinus G. J. Van Beuningen, Tim Kievits, Thomas A. M. Beumer, Akzo Nobel Nv, Biomerieux B.V. filed Critical Marinus G. J. Van Beuningen
Publication of CA2143857A1 publication Critical patent/CA2143857A1/en
Application granted granted Critical
Publication of CA2143857C publication Critical patent/CA2143857C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is related to an improved method for the quantification of nucleic acid, which can be performed with a minimal amount of nucleic acid amplification reactions. The method according to the invention for the quantification of analyte nucleic acid in a sample comprises the steps of adding to the sample different respective amounts of different nucleic acid constructs, each construct being distinguishable from the analyte nucleic acid and capable of being co-amplified with the analyte nucleic acid; subjecting the sample to a nucleic acid amplification procedure, using amplification reagents capable of reacting with both the analyte nucleic acid and the nucleic acid constructs; detecting the relative amounts of amplificates derived from analyte nucleic acid and each nucleic acid construct; calculating the amount of analyte nucleic acid from said relative amounts. Each nucleic acid construct is different; the nucleic acid constructs can be distinguished from one another and from the analyte nuclic acid. The nucleic acid constructs do resemble each other, and the analyte nucleic acid, in that all an capable of reacting with the same amplification reagents.
CA002143857A 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid Expired - Lifetime CA2143857C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP93202015 1993-07-09
EP93202015.9 1993-07-09
EP93203424.2 1993-12-06
EP93203424 1993-12-06
PCT/EP1994/002295 WO1995002067A1 (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Publications (2)

Publication Number Publication Date
CA2143857A1 true CA2143857A1 (en) 1995-01-19
CA2143857C CA2143857C (en) 2005-09-20

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002143857A Expired - Lifetime CA2143857C (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Country Status (10)

Country Link
JP (1) JP3594598B2 (en)
KR (1) KR100312800B1 (en)
AT (1) ATE178946T1 (en)
AU (1) AU668659B2 (en)
CA (1) CA2143857C (en)
DE (1) DE69417863T2 (en)
DK (1) DK0662156T3 (en)
ES (1) ES2131699T3 (en)
FI (1) FI112094B (en)
WO (1) WO1995002067A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09505464A (en) * 1993-09-22 1997-06-03 イゲン，インコーポレーテッド Autonomous sequence replication electrochemiluminescence nucleic acid assay
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1996040990A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (en) * 1997-07-01 2002-08-09 Pasteur Institut SEMI-QUANTIFICATION METHOD OF WHEELS
AU4515699A (en) * 1998-06-26 2000-01-17 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (en) * 1998-12-22 2000-06-28 Universiteit van Amsterdam A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
AU781570B2 (en) * 1999-07-23 2005-06-02 Gen-Probe Incorporated Polynucleotide amplification method
AT409383B (en) * 1999-12-22 2002-07-25 Baxter Ag METHOD FOR DETECTING AND QUANTIFYING NUCLEIC ACIDS IN A SAMPLE
WO2002024949A1 (en) 2000-09-22 2002-03-28 Hitachi, Ltd. Method of analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (en) * 2009-01-06 2014-08-06 株式会社島津製作所 DNA quantification method and gene analysis method
JP2011062119A (en) 2009-09-16 2011-03-31 Seiko Epson Corp Chip for quantitatively determining biological sample
CA2828192A1 (en) 2011-02-25 2012-08-30 Novartis Ag Exogenous internal positive control
KR102108855B1 (en) 2017-10-30 2020-05-12 한국표준과학연구원 Method for quantification of nucleic acids wherein stable isotope-labelled nucleic acids are used as internal standards and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
DE69233285T2 (en) * 1991-08-02 2004-11-25 bioMérieux B.V. Quantification of nucleic acids
FR2683827B1 (en) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic METHOD FOR DETERMINING THE QUANTITY OF A FRAGMENT OF DNA OF INTEREST BY AN ENZYMATIC AMPLIFICATION METHOD.

Also Published As

Publication number Publication date
ATE178946T1 (en) 1999-04-15
JPH08501222A (en) 1996-02-13
DE69417863T2 (en) 1999-10-07
CA2143857C (en) 2005-09-20
FI951082A (en) 1995-03-08
ES2131699T3 (en) 1999-08-01
WO1995002067A1 (en) 1995-01-19
JP3594598B2 (en) 2004-12-02
FI951082A0 (en) 1995-03-08
DK0662156T3 (en) 1999-10-25
KR100312800B1 (en) 2002-02-28
AU668659B2 (en) 1996-05-09
AU7492894A (en) 1995-02-06
FI112094B (en) 2003-10-31
DE69417863D1 (en) 1999-05-20

Similar Documents

Publication Publication Date Title
CA2143857A1 (en) Improved method for the quantification of nucleic acid
CA2143202C (en) Elimination of false negatives in nucleic acid detection
EP0714987A3 (en) Method for quantifying genomic DNA
CA2291440A1 (en) Length determination of nucleic acid repeat sequences by discontinuous primer extension
AU5442896A (en) Methods and reagents for combined PCR amplification and hybridization probing assay
CA2035010A1 (en) Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
CA2212185A1 (en) Methods for the isothermal amplification of nucleic acid molecules
CA2230967A1 (en) Improved nucleic acid assays
EP0379369A3 (en) Nucleic acid amplification using single primer
PL278176A1 (en) Method of detecting sequences of nucleotides and sets for carrying out diagnostic tests
CA2153944A1 (en) Method for the sensitive detection of nucleic acids
EP0932700A1 (en) Method of sequencing dna
WO1999005315A3 (en) Nucleic acid sequence analysis
NZ287118A (en) Method of detecting and amplifying oligonucleotides
EP0374665A3 (en) Assay of sequences using amplified genes
EP0861332A1 (en) A method of sequencing
AU2623197A (en) Method for the detection of telomerase activity
EP0892070A3 (en) Integrated process and system for the amplification and detection of nucleic acids
CA2052413A1 (en) Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods
WO2002090586A3 (en) Analytical method and kit
CA2093647A1 (en) Method for the sensitive detection of nucleic acids
EP0751226A3 (en) Process for amplifying nucleic acid sequences
WO2000012756A3 (en) Reaction chambers coated with defined concentrations of nucleic acids, method for the production and use thereof
CA2211165A1 (en) Methods for sensitive detection of reverse transcriptase
CA2100122A1 (en) Improved method for rapidly detecting non-viral organisms

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20140708