CA2093647A1 - Method for the sensitive detection of nucleic acids - Google Patents

Method for the sensitive detection of nucleic acids

Info

Publication number
CA2093647A1
CA2093647A1 CA2093647A CA2093647A CA2093647A1 CA 2093647 A1 CA2093647 A1 CA 2093647A1 CA 2093647 A CA2093647 A CA 2093647A CA 2093647 A CA2093647 A CA 2093647A CA 2093647 A1 CA2093647 A1 CA 2093647A1
Authority
CA
Canada
Prior art keywords
nucleic acid
labelled
solid phase
acid probe
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2093647A
Other languages
French (fr)
Other versions
CA2093647C (en
Inventor
Christoph Kessler
Ruediger Rueger
Rudolf Seibl
Cornelia Kruse-Mueller
Sibylle Berner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4038804A external-priority patent/DE4038804A1/en
Priority claimed from DE4041608A external-priority patent/DE4041608A1/en
Application filed by Individual filed Critical Individual
Publication of CA2093647A1 publication Critical patent/CA2093647A1/en
Application granted granted Critical
Publication of CA2093647C publication Critical patent/CA2093647C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Control Of El Displays (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a method for the specific detection of a nucleic acid A in a sample which comprises the following steps in the given sequence: a) reacting the sample with one or several labelled mononucleoside triphosphates and one or several enzymes which catalyze the production of a labelled nucleic acid B which contains said labelled mononucleoside triphosphates, b) non-thermal denaturation of the reaction mixture subsequent to step a), c) hybridization of the nucleic acid B with a nucleic acid probe C which contains at least one immobilizable group and which is sufficiently complementary to the nucleic acid B, and simultaneous immobilization of the nucleic acid probe C to a solid phase which can bind the immobilizable nucleic acid probe C, d) separating the liquid phase from the solid phase, and e) detecting the nucleic acid hybrid D formed from the labelled nucleic acid B and nucleic acid probe C
by detecting the detectable group bound to the solid phase.
CA002093647A 1990-10-09 1991-10-04 Method for the sensitive detection of nucleic acids Expired - Lifetime CA2093647C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DEP4032024.3 1990-10-09
DE4032024 1990-10-09
DEP4038804.2 1990-12-05
DE4038804A DE4038804A1 (en) 1990-10-09 1990-12-05 METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID
DEP4041608.9 1990-12-22
DE4041608A DE4041608A1 (en) 1990-12-22 1990-12-22 Sensitive and selective nucleic acid detection
PCT/EP1991/001898 WO1992006216A1 (en) 1990-10-09 1991-10-04 Nucleic acid sensitive detection process

Publications (2)

Publication Number Publication Date
CA2093647A1 true CA2093647A1 (en) 1992-04-10
CA2093647C CA2093647C (en) 2000-09-19

Family

ID=27201764

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002093647A Expired - Lifetime CA2093647C (en) 1990-10-09 1991-10-04 Method for the sensitive detection of nucleic acids

Country Status (15)

Country Link
EP (1) EP0552203B1 (en)
JP (1) JP2673162B2 (en)
AT (1) ATE122104T1 (en)
AU (1) AU655781B2 (en)
CA (1) CA2093647C (en)
DE (1) DE59105402D1 (en)
DK (1) DK0552203T3 (en)
ES (1) ES2073771T3 (en)
FI (1) FI103808B1 (en)
IE (1) IE68525B1 (en)
IL (1) IL99663A (en)
NO (1) NO305995B1 (en)
NZ (1) NZ240079A (en)
PT (1) PT99177B (en)
WO (1) WO1992006216A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9677127B2 (en) 2002-11-07 2017-06-13 Sekisui Medical Co., Ltd. Method of detecting gene mutation

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0702728B1 (en) * 1993-06-09 1998-04-15 Gamera Bioscience Corporation Magnetic cycle reaction
DE4344742A1 (en) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Method for the immobilization of nucleic acids
WO1997038131A1 (en) * 1996-04-11 1997-10-16 Valentina Filippovna Kulikova Method for detecting a nucleic acid chain to be analysed
WO2000068422A2 (en) * 1999-05-07 2000-11-16 Roche Diagnostics Gmbh High density labeling of dna with modified or 'chromophore' carrying nucleotides and dna polymerases used

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA862334B (en) * 1985-03-28 1987-11-25 Cetus Corp Process for amplifying,detecting and/or cloning nucleic acid sequences
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2650159B2 (en) * 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー Nucleic acid amplification method
AT390080B (en) * 1988-07-29 1990-03-12 Lion Thomas Dr FAST BLOT METHOD FOR DETECTING VIRAL SEQUENCES AND AMPLIFIED GENE SEQUENCES IN HUMAN CELLS
CA2004326A1 (en) * 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
KR920700360A (en) * 1989-03-22 1992-02-19 하리크 프리드리히 Sliding bearing
CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9677127B2 (en) 2002-11-07 2017-06-13 Sekisui Medical Co., Ltd. Method of detecting gene mutation

Also Published As

Publication number Publication date
AU8627591A (en) 1992-04-28
JP2673162B2 (en) 1997-11-05
WO1992006216A1 (en) 1992-04-16
PT99177A (en) 1992-09-30
FI103808B (en) 1999-09-30
FI931586A0 (en) 1993-04-07
NO305995B1 (en) 1999-08-30
EP0552203A1 (en) 1993-07-28
ES2073771T3 (en) 1995-08-16
NO931309D0 (en) 1993-04-06
NO931309L (en) 1993-04-06
FI103808B1 (en) 1999-09-30
PT99177B (en) 1999-04-30
IL99663A (en) 1995-12-31
FI931586L (en) 1993-04-07
DK0552203T3 (en) 1995-10-02
AU655781B2 (en) 1995-01-12
DE59105402D1 (en) 1995-06-08
IL99663A0 (en) 1992-08-18
IE913552A1 (en) 1992-04-22
IE68525B1 (en) 1996-06-26
ATE122104T1 (en) 1995-05-15
CA2093647C (en) 2000-09-19
NZ240079A (en) 1993-07-27
JPH06501157A (en) 1994-02-10
EP0552203B1 (en) 1995-05-03

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Legal Events

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