DK0552203T3 - Method for Sensitive Detection of Nucleic Acid - Google Patents
Method for Sensitive Detection of Nucleic AcidInfo
- Publication number
- DK0552203T3 DK0552203T3 DK91917451.6T DK91917451T DK0552203T3 DK 0552203 T3 DK0552203 T3 DK 0552203T3 DK 91917451 T DK91917451 T DK 91917451T DK 0552203 T3 DK0552203 T3 DK 0552203T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acid
- sample
- solid phase
- reacted
- sensitive detection
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Abstract
A process is disclosed for specifically detecting nucleic acids in a sample. The sample is reacted with one or several labeled nucleotide triphosphates and one or several enzymes which catalyze the production of a labeled nucleic acid B containing said nucleotide. Then the sample is subjected to a non thermal denaturation and reacted with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group. The possibly formed nucleic acid hybrid D is contacted with a solid phase which recognizes and binds the immobilizable group, the liquid phase is removed from the solid phase and the labeling of the solid phase is considered as a measure of the presence of nucleic acids.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4032024 | 1990-10-09 | ||
DE4038804A DE4038804A1 (en) | 1990-10-09 | 1990-12-05 | METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID |
DE4041608A DE4041608A1 (en) | 1990-12-22 | 1990-12-22 | Sensitive and selective nucleic acid detection |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0552203T3 true DK0552203T3 (en) | 1995-10-02 |
Family
ID=27201764
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK91917451.6T DK0552203T3 (en) | 1990-10-09 | 1991-10-04 | Method for Sensitive Detection of Nucleic Acid |
Country Status (15)
Country | Link |
---|---|
EP (1) | EP0552203B1 (en) |
JP (1) | JP2673162B2 (en) |
AT (1) | ATE122104T1 (en) |
AU (1) | AU655781B2 (en) |
CA (1) | CA2093647C (en) |
DE (1) | DE59105402D1 (en) |
DK (1) | DK0552203T3 (en) |
ES (1) | ES2073771T3 (en) |
FI (1) | FI103808B (en) |
IE (1) | IE68525B1 (en) |
IL (1) | IL99663A (en) |
NO (1) | NO305995B1 (en) |
NZ (1) | NZ240079A (en) |
PT (1) | PT99177B (en) |
WO (1) | WO1992006216A1 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4344742A1 (en) * | 1993-06-09 | 1994-12-15 | Boehringer Mannheim Gmbh | Method for the immobilization of nucleic acids |
AU690124B2 (en) * | 1993-06-09 | 1998-04-23 | Gamera Bioscience Corporation | Magnetic cycle reaction |
RU2146707C1 (en) * | 1996-04-11 | 2000-03-20 | Куликова Валентина Филипповна | Method of detection of analyzed sequence of nucleic acid |
DE60024464T2 (en) * | 1999-05-07 | 2006-08-03 | Roche Diagnostics Gmbh | HIGH DENSITY MARKING OF DNA WITH MODIFIED OR CHROMOPHORE-CARRYING NUCLEOTIDES AND USED DNA POLYMERASES |
CA2506654C (en) | 2002-11-07 | 2014-02-25 | Yoichi Matsubara | Method of detecting gene mutation |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
AT390080B (en) * | 1988-07-29 | 1990-03-12 | Lion Thomas Dr | FAST BLOT METHOD FOR DETECTING VIRAL SEQUENCES AND AMPLIFIED GENE SEQUENCES IN HUMAN CELLS |
CA2004326A1 (en) * | 1988-12-23 | 1990-06-23 | Nanibushan Dattagupta | Assay of sequences using amplified genes |
KR920700360A (en) * | 1989-03-22 | 1992-02-19 | 하리크 프리드리히 | Sliding bearing |
CA2012982A1 (en) * | 1989-03-27 | 1990-09-27 | Frank W. Hobbs | Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid |
US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
-
1991
- 1991-10-03 NZ NZ240079A patent/NZ240079A/en unknown
- 1991-10-04 EP EP91917451A patent/EP0552203B1/en not_active Expired - Lifetime
- 1991-10-04 WO PCT/EP1991/001898 patent/WO1992006216A1/en active IP Right Grant
- 1991-10-04 DK DK91917451.6T patent/DK0552203T3/en active
- 1991-10-04 AT AT91917451T patent/ATE122104T1/en not_active IP Right Cessation
- 1991-10-04 CA CA002093647A patent/CA2093647C/en not_active Expired - Lifetime
- 1991-10-04 ES ES91917451T patent/ES2073771T3/en not_active Expired - Lifetime
- 1991-10-04 JP JP3515944A patent/JP2673162B2/en not_active Expired - Lifetime
- 1991-10-04 AU AU86275/91A patent/AU655781B2/en not_active Expired
- 1991-10-04 DE DE59105402T patent/DE59105402D1/en not_active Expired - Lifetime
- 1991-10-07 IL IL9966391A patent/IL99663A/en not_active IP Right Cessation
- 1991-10-08 PT PT99177A patent/PT99177B/en not_active IP Right Cessation
- 1991-10-16 IE IE355291A patent/IE68525B1/en not_active IP Right Cessation
-
1993
- 1993-04-06 NO NO931309A patent/NO305995B1/en not_active IP Right Cessation
- 1993-04-07 FI FI931586A patent/FI103808B/en active
Also Published As
Publication number | Publication date |
---|---|
IL99663A0 (en) | 1992-08-18 |
FI931586A0 (en) | 1993-04-07 |
NO931309D0 (en) | 1993-04-06 |
ATE122104T1 (en) | 1995-05-15 |
ES2073771T3 (en) | 1995-08-16 |
CA2093647A1 (en) | 1992-04-10 |
AU8627591A (en) | 1992-04-28 |
WO1992006216A1 (en) | 1992-04-16 |
NO931309L (en) | 1993-04-06 |
AU655781B2 (en) | 1995-01-12 |
IE913552A1 (en) | 1992-04-22 |
NO305995B1 (en) | 1999-08-30 |
DE59105402D1 (en) | 1995-06-08 |
IL99663A (en) | 1995-12-31 |
EP0552203B1 (en) | 1995-05-03 |
FI931586A (en) | 1993-04-07 |
PT99177B (en) | 1999-04-30 |
CA2093647C (en) | 2000-09-19 |
PT99177A (en) | 1992-09-30 |
FI103808B1 (en) | 1999-09-30 |
EP0552203A1 (en) | 1993-07-28 |
IE68525B1 (en) | 1996-06-26 |
NZ240079A (en) | 1993-07-27 |
JPH06501157A (en) | 1994-02-10 |
FI103808B (en) | 1999-09-30 |
JP2673162B2 (en) | 1997-11-05 |
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